Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange

Set2-mediated methylation of histone H3 Lys36 (H3K36) is a mark associated with the coding sequences of actively transcribed genes, yet plays a negative role during transcription elongation. It prevents trans-histone exchange over coding regions and signals for histone deacetylation in the wake of R...

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Autores principales: Smolle, Michaela, Venkatesh, Swaminathan, Gogol, Madelaine M., Li, Hua, Zhang, Ying, Florens, Laurence, Washburn, Michael P., Workman, Jerry L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560298/
https://www.ncbi.nlm.nih.gov/pubmed/22922743
http://dx.doi.org/10.1038/nsmb.2312
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author Smolle, Michaela
Venkatesh, Swaminathan
Gogol, Madelaine M.
Li, Hua
Zhang, Ying
Florens, Laurence
Washburn, Michael P.
Workman, Jerry L.
author_facet Smolle, Michaela
Venkatesh, Swaminathan
Gogol, Madelaine M.
Li, Hua
Zhang, Ying
Florens, Laurence
Washburn, Michael P.
Workman, Jerry L.
author_sort Smolle, Michaela
collection PubMed
description Set2-mediated methylation of histone H3 Lys36 (H3K36) is a mark associated with the coding sequences of actively transcribed genes, yet plays a negative role during transcription elongation. It prevents trans-histone exchange over coding regions and signals for histone deacetylation in the wake of RNA polymerase II (RNAPII) passage. We have found that in Saccharomyces cerevisiae the Isw1b chromatin-remodeling complex is specifically recruited to open reading frames (ORFs) by H3K36 methylation through the PWWP domain of its Ioc4 subunit in vivo and in vitro. Isw1b acts in conjunction with Chd1 to regulate chromatin structure by preventing trans-histone exchange from taking place over coding regions and thus maintains chromatin integrity during transcription elongation by RNA polymerase II.
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spelling pubmed-35602982013-03-01 Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange Smolle, Michaela Venkatesh, Swaminathan Gogol, Madelaine M. Li, Hua Zhang, Ying Florens, Laurence Washburn, Michael P. Workman, Jerry L. Nat Struct Mol Biol Article Set2-mediated methylation of histone H3 Lys36 (H3K36) is a mark associated with the coding sequences of actively transcribed genes, yet plays a negative role during transcription elongation. It prevents trans-histone exchange over coding regions and signals for histone deacetylation in the wake of RNA polymerase II (RNAPII) passage. We have found that in Saccharomyces cerevisiae the Isw1b chromatin-remodeling complex is specifically recruited to open reading frames (ORFs) by H3K36 methylation through the PWWP domain of its Ioc4 subunit in vivo and in vitro. Isw1b acts in conjunction with Chd1 to regulate chromatin structure by preventing trans-histone exchange from taking place over coding regions and thus maintains chromatin integrity during transcription elongation by RNA polymerase II. 2012-08-26 2012-09 /pmc/articles/PMC3560298/ /pubmed/22922743 http://dx.doi.org/10.1038/nsmb.2312 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Smolle, Michaela
Venkatesh, Swaminathan
Gogol, Madelaine M.
Li, Hua
Zhang, Ying
Florens, Laurence
Washburn, Michael P.
Workman, Jerry L.
Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange
title Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange
title_full Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange
title_fullStr Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange
title_full_unstemmed Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange
title_short Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange
title_sort chromatin remodelers isw1 and chd1 maintain chromatin structure during transcription by preventing histone exchange
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560298/
https://www.ncbi.nlm.nih.gov/pubmed/22922743
http://dx.doi.org/10.1038/nsmb.2312
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