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Control of Candida albicans Metabolism and Biofilm Formation by Pseudomonas aeruginosa Phenazines
Candida albicans has developmental programs that govern transitions between yeast and filamentous morphologies and between unattached and biofilm lifestyles. Here, we report that filamentation, intercellular adherence, and biofilm development were inhibited during interactions between Candida albica...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560528/ https://www.ncbi.nlm.nih.gov/pubmed/23362320 http://dx.doi.org/10.1128/mBio.00526-12 |
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author | Morales, Diana K. Grahl, Nora Okegbe, Chinweike Dietrich, Lars E. P. Jacobs, Nicholas J. Hogan, Deborah A. |
author_facet | Morales, Diana K. Grahl, Nora Okegbe, Chinweike Dietrich, Lars E. P. Jacobs, Nicholas J. Hogan, Deborah A. |
author_sort | Morales, Diana K. |
collection | PubMed |
description | Candida albicans has developmental programs that govern transitions between yeast and filamentous morphologies and between unattached and biofilm lifestyles. Here, we report that filamentation, intercellular adherence, and biofilm development were inhibited during interactions between Candida albicans and Pseudomonas aeruginosa through the action of P. aeruginosa-produced phenazines. While phenazines are toxic to C. albicans at millimolar concentrations, we found that lower concentrations of any of three different phenazines (pyocyanin, phenazine methosulfate, and phenazine-1-carboxylate) allowed growth but affected the development of C. albicans wrinkled colony biofilms and inhibited the fungal yeast-to-filament transition. Phenazines impaired C. albicans growth on nonfermentable carbon sources and led to increased production of fermentation products (ethanol, glycerol, and acetate) in glucose-containing medium, leading us to propose that phenazines specifically inhibited respiration. Methylene blue, another inhibitor of respiration, also prevented the formation of structured colony biofilms. The inhibition of filamentation and colony wrinkling was not solely due to lowered extracellular pH induced by fermentation. Compared to smooth, unstructured colonies, wrinkled colony biofilms had higher oxygen concentrations within the colony, and wrinkled regions of these colonies had higher levels of respiration. Together, our data suggest that the structure of the fungal biofilm promotes access to oxygen and enhances respiratory metabolism and that the perturbation of respiration by bacterial molecules such as phenazines or compounds with similar activities disrupts these pathways. These findings may suggest new ways to limit fungal biofilms in the context of disease. |
format | Online Article Text |
id | pubmed-3560528 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | American Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-35605282013-02-09 Control of Candida albicans Metabolism and Biofilm Formation by Pseudomonas aeruginosa Phenazines Morales, Diana K. Grahl, Nora Okegbe, Chinweike Dietrich, Lars E. P. Jacobs, Nicholas J. Hogan, Deborah A. mBio Research Article Candida albicans has developmental programs that govern transitions between yeast and filamentous morphologies and between unattached and biofilm lifestyles. Here, we report that filamentation, intercellular adherence, and biofilm development were inhibited during interactions between Candida albicans and Pseudomonas aeruginosa through the action of P. aeruginosa-produced phenazines. While phenazines are toxic to C. albicans at millimolar concentrations, we found that lower concentrations of any of three different phenazines (pyocyanin, phenazine methosulfate, and phenazine-1-carboxylate) allowed growth but affected the development of C. albicans wrinkled colony biofilms and inhibited the fungal yeast-to-filament transition. Phenazines impaired C. albicans growth on nonfermentable carbon sources and led to increased production of fermentation products (ethanol, glycerol, and acetate) in glucose-containing medium, leading us to propose that phenazines specifically inhibited respiration. Methylene blue, another inhibitor of respiration, also prevented the formation of structured colony biofilms. The inhibition of filamentation and colony wrinkling was not solely due to lowered extracellular pH induced by fermentation. Compared to smooth, unstructured colonies, wrinkled colony biofilms had higher oxygen concentrations within the colony, and wrinkled regions of these colonies had higher levels of respiration. Together, our data suggest that the structure of the fungal biofilm promotes access to oxygen and enhances respiratory metabolism and that the perturbation of respiration by bacterial molecules such as phenazines or compounds with similar activities disrupts these pathways. These findings may suggest new ways to limit fungal biofilms in the context of disease. American Society of Microbiology 2013-01-29 /pmc/articles/PMC3560528/ /pubmed/23362320 http://dx.doi.org/10.1128/mBio.00526-12 Text en Copyright © 2013 Morales et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported (http://creativecommons.org/licenses/by-nc-sa/3.0/) license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Morales, Diana K. Grahl, Nora Okegbe, Chinweike Dietrich, Lars E. P. Jacobs, Nicholas J. Hogan, Deborah A. Control of Candida albicans Metabolism and Biofilm Formation by Pseudomonas aeruginosa Phenazines |
title | Control of Candida albicans Metabolism and Biofilm Formation by Pseudomonas aeruginosa Phenazines |
title_full | Control of Candida albicans Metabolism and Biofilm Formation by Pseudomonas aeruginosa Phenazines |
title_fullStr | Control of Candida albicans Metabolism and Biofilm Formation by Pseudomonas aeruginosa Phenazines |
title_full_unstemmed | Control of Candida albicans Metabolism and Biofilm Formation by Pseudomonas aeruginosa Phenazines |
title_short | Control of Candida albicans Metabolism and Biofilm Formation by Pseudomonas aeruginosa Phenazines |
title_sort | control of candida albicans metabolism and biofilm formation by pseudomonas aeruginosa phenazines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560528/ https://www.ncbi.nlm.nih.gov/pubmed/23362320 http://dx.doi.org/10.1128/mBio.00526-12 |
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