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Lack of association of conjunctival MALT lymphoma with Chlamydiae or Helicobacter pylori in a cohort of Chinese patients

BACKGROUND: This study was conducted to detect microbial pathogens in conjunctival mucosa-associated lymphoid tissue (MALT) lymphoma specimens in an attempt to determine possible associations between conjunctival MALT lymphoma and microbial infections. MATERIAL/METHODS: Using PCR technique, freshly...

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Autores principales: Cai, Ji-Ping, Cheng, Jin-Wei, Ma, Xiao-Ye, Li, Yu-Zhen, Li, You, Huang, Xiao, Wei, Rui-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560575/
https://www.ncbi.nlm.nih.gov/pubmed/22293871
http://dx.doi.org/10.12659/MSM.882462
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author Cai, Ji-Ping
Cheng, Jin-Wei
Ma, Xiao-Ye
Li, Yu-Zhen
Li, You
Huang, Xiao
Wei, Rui-Li
author_facet Cai, Ji-Ping
Cheng, Jin-Wei
Ma, Xiao-Ye
Li, Yu-Zhen
Li, You
Huang, Xiao
Wei, Rui-Li
author_sort Cai, Ji-Ping
collection PubMed
description BACKGROUND: This study was conducted to detect microbial pathogens in conjunctival mucosa-associated lymphoid tissue (MALT) lymphoma specimens in an attempt to determine possible associations between conjunctival MALT lymphoma and microbial infections. MATERIAL/METHODS: Using PCR technique, freshly obtained tumor specimens from 16 cases of conjunctival MALT lymphoma, as confirmed by postoperative pathology, were analyzed for DNA of Chlamydia psittaci (C. psittaci), Chlamydia trachomatis (C. trachomatis), Chlamydia pneumoniae (C. pneumoniae) and Helicobacter pylori (H. pylori). Synthetic C. psittaci, C. trachomatis, C. pneumoniae and H. pylori DNA were used as positive control, and blank plasmid DNA as negative control. RESULTS: Electrophoresis showed that no bands corresponding to the positive control were observed in the specimens, indicating that no DNA of the 4 microorganisms was detected in the specimens of the 16 cases of conjunctival MALT lymphoma. CONCLUSIONS: The PCR technique was able to detect the positive control quickly and accurately, but the results of PCR in analyzing the 16 specimens were negative, indicating that there is no association between conjunctival MALT lymphoma and the 4 microorganisms in Chinese patients.
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spelling pubmed-35605752013-04-24 Lack of association of conjunctival MALT lymphoma with Chlamydiae or Helicobacter pylori in a cohort of Chinese patients Cai, Ji-Ping Cheng, Jin-Wei Ma, Xiao-Ye Li, Yu-Zhen Li, You Huang, Xiao Wei, Rui-Li Med Sci Monit Basic Research BACKGROUND: This study was conducted to detect microbial pathogens in conjunctival mucosa-associated lymphoid tissue (MALT) lymphoma specimens in an attempt to determine possible associations between conjunctival MALT lymphoma and microbial infections. MATERIAL/METHODS: Using PCR technique, freshly obtained tumor specimens from 16 cases of conjunctival MALT lymphoma, as confirmed by postoperative pathology, were analyzed for DNA of Chlamydia psittaci (C. psittaci), Chlamydia trachomatis (C. trachomatis), Chlamydia pneumoniae (C. pneumoniae) and Helicobacter pylori (H. pylori). Synthetic C. psittaci, C. trachomatis, C. pneumoniae and H. pylori DNA were used as positive control, and blank plasmid DNA as negative control. RESULTS: Electrophoresis showed that no bands corresponding to the positive control were observed in the specimens, indicating that no DNA of the 4 microorganisms was detected in the specimens of the 16 cases of conjunctival MALT lymphoma. CONCLUSIONS: The PCR technique was able to detect the positive control quickly and accurately, but the results of PCR in analyzing the 16 specimens were negative, indicating that there is no association between conjunctival MALT lymphoma and the 4 microorganisms in Chinese patients. International Scientific Literature, Inc. 2012-02-01 /pmc/articles/PMC3560575/ /pubmed/22293871 http://dx.doi.org/10.12659/MSM.882462 Text en © Med Sci Monit, 2012 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
spellingShingle Basic Research
Cai, Ji-Ping
Cheng, Jin-Wei
Ma, Xiao-Ye
Li, Yu-Zhen
Li, You
Huang, Xiao
Wei, Rui-Li
Lack of association of conjunctival MALT lymphoma with Chlamydiae or Helicobacter pylori in a cohort of Chinese patients
title Lack of association of conjunctival MALT lymphoma with Chlamydiae or Helicobacter pylori in a cohort of Chinese patients
title_full Lack of association of conjunctival MALT lymphoma with Chlamydiae or Helicobacter pylori in a cohort of Chinese patients
title_fullStr Lack of association of conjunctival MALT lymphoma with Chlamydiae or Helicobacter pylori in a cohort of Chinese patients
title_full_unstemmed Lack of association of conjunctival MALT lymphoma with Chlamydiae or Helicobacter pylori in a cohort of Chinese patients
title_short Lack of association of conjunctival MALT lymphoma with Chlamydiae or Helicobacter pylori in a cohort of Chinese patients
title_sort lack of association of conjunctival malt lymphoma with chlamydiae or helicobacter pylori in a cohort of chinese patients
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560575/
https://www.ncbi.nlm.nih.gov/pubmed/22293871
http://dx.doi.org/10.12659/MSM.882462
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