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Effect of heat treatment on H(2)O(2)/HCl etched pure titanium dental implant: An in vitro study
BACKGROUND: Surface chemistry of dental implant plays an important role in osseointegration. Heat treatment might alter surface chemistry and result in different biological response. The aim of this study was to investigate the roles of heat treatment of H(2)O(2)/HCl-treated Ti implants in cell atta...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560775/ https://www.ncbi.nlm.nih.gov/pubmed/22739726 http://dx.doi.org/10.12659/MSM.883204 |
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author | Zhang, Feng Zhang, Chun-Fei Yin, Mei-nv Ren, Ling-Fei Lin, Hai-sheng Shi, Geng-sheng |
author_facet | Zhang, Feng Zhang, Chun-Fei Yin, Mei-nv Ren, Ling-Fei Lin, Hai-sheng Shi, Geng-sheng |
author_sort | Zhang, Feng |
collection | PubMed |
description | BACKGROUND: Surface chemistry of dental implant plays an important role in osseointegration. Heat treatment might alter surface chemistry and result in different biological response. The aim of this study was to investigate the roles of heat treatment of H(2)O(2)/HCl-treated Ti implants in cell attachment, proliferation and osteoblastic differentiation. MATERIAL/METHODS: Sandblasted, dual acid-etched and H(2)O(2)/HCl heat-treated discs were set as the control group and sandblasted, dual acid-etched H(2)O(2)/HCl-treated discs were the test group. Both groups’ discs were sent for surface characterization. MC3T3-E1 cells were seeded on these 2 groups’ discs for 3 hours to 14 days, and then cell attachment, cell proliferation and cell differentiation were evaluated. RESULTS: Scanning electron microscope analysis revealed that the titanium discs in the 2 groups shared the same surface topography, while x-ray diffraction examination showed an anatase layer in the control group and titanium hydride diffractions in the test group. The cell attachment of the test group was equivalent to that of the control group. Cell proliferation was slightly stimulated at all time points in the control group, but the alkaline phosphatase (ALP) activity and osteocalcin (OC) production increased significantly in the test group compared with those in the control group at every time point investigated (p<0.05 or p<0.01). Moreover, the osteoblastic differentiation-related genes AKP-2, osteopontin (OPN) and OC were greatly up-regulated in the test group (p<0.05 or p<0.01). CONCLUSIONS: The results implied that surface chemistry played an important role in cell response, and H(2)O(2)/HCl etched titanium surface without subsequent heat treatment might improve osseointegration response. |
format | Online Article Text |
id | pubmed-3560775 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-35607752013-04-24 Effect of heat treatment on H(2)O(2)/HCl etched pure titanium dental implant: An in vitro study Zhang, Feng Zhang, Chun-Fei Yin, Mei-nv Ren, Ling-Fei Lin, Hai-sheng Shi, Geng-sheng Med Sci Monit Basic Research BACKGROUND: Surface chemistry of dental implant plays an important role in osseointegration. Heat treatment might alter surface chemistry and result in different biological response. The aim of this study was to investigate the roles of heat treatment of H(2)O(2)/HCl-treated Ti implants in cell attachment, proliferation and osteoblastic differentiation. MATERIAL/METHODS: Sandblasted, dual acid-etched and H(2)O(2)/HCl heat-treated discs were set as the control group and sandblasted, dual acid-etched H(2)O(2)/HCl-treated discs were the test group. Both groups’ discs were sent for surface characterization. MC3T3-E1 cells were seeded on these 2 groups’ discs for 3 hours to 14 days, and then cell attachment, cell proliferation and cell differentiation were evaluated. RESULTS: Scanning electron microscope analysis revealed that the titanium discs in the 2 groups shared the same surface topography, while x-ray diffraction examination showed an anatase layer in the control group and titanium hydride diffractions in the test group. The cell attachment of the test group was equivalent to that of the control group. Cell proliferation was slightly stimulated at all time points in the control group, but the alkaline phosphatase (ALP) activity and osteocalcin (OC) production increased significantly in the test group compared with those in the control group at every time point investigated (p<0.05 or p<0.01). Moreover, the osteoblastic differentiation-related genes AKP-2, osteopontin (OPN) and OC were greatly up-regulated in the test group (p<0.05 or p<0.01). CONCLUSIONS: The results implied that surface chemistry played an important role in cell response, and H(2)O(2)/HCl etched titanium surface without subsequent heat treatment might improve osseointegration response. International Scientific Literature, Inc. 2012-07-01 /pmc/articles/PMC3560775/ /pubmed/22739726 http://dx.doi.org/10.12659/MSM.883204 Text en © Med Sci Monit, 2012 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. |
spellingShingle | Basic Research Zhang, Feng Zhang, Chun-Fei Yin, Mei-nv Ren, Ling-Fei Lin, Hai-sheng Shi, Geng-sheng Effect of heat treatment on H(2)O(2)/HCl etched pure titanium dental implant: An in vitro study |
title | Effect of heat treatment on H(2)O(2)/HCl etched pure titanium dental implant: An in vitro study |
title_full | Effect of heat treatment on H(2)O(2)/HCl etched pure titanium dental implant: An in vitro study |
title_fullStr | Effect of heat treatment on H(2)O(2)/HCl etched pure titanium dental implant: An in vitro study |
title_full_unstemmed | Effect of heat treatment on H(2)O(2)/HCl etched pure titanium dental implant: An in vitro study |
title_short | Effect of heat treatment on H(2)O(2)/HCl etched pure titanium dental implant: An in vitro study |
title_sort | effect of heat treatment on h(2)o(2)/hcl etched pure titanium dental implant: an in vitro study |
topic | Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560775/ https://www.ncbi.nlm.nih.gov/pubmed/22739726 http://dx.doi.org/10.12659/MSM.883204 |
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