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Extracellular matrix in deoxycholic acid decellularized aortic heart valves

BACKGROUND: Only limited information is available regarding the influence of decellularization on the extracellular matrix in heart valves. Within the extracellular matrix proteoglycans (PG) play a central role in the structural organization and physical functioning of valves and in their capability...

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Autores principales: Bloch, Oliver, Erdbrügger, Wilhelm, Völker, Wolfgang, Schenk, Alexander, Posner, Steffen, Konertz, Wolfgang, Dohmen, Pascal M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560792/
https://www.ncbi.nlm.nih.gov/pubmed/23207452
http://dx.doi.org/10.12659/MSM.883618
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author Bloch, Oliver
Erdbrügger, Wilhelm
Völker, Wolfgang
Schenk, Alexander
Posner, Steffen
Konertz, Wolfgang
Dohmen, Pascal M.
author_facet Bloch, Oliver
Erdbrügger, Wilhelm
Völker, Wolfgang
Schenk, Alexander
Posner, Steffen
Konertz, Wolfgang
Dohmen, Pascal M.
author_sort Bloch, Oliver
collection PubMed
description BACKGROUND: Only limited information is available regarding the influence of decellularization on the extracellular matrix in heart valves. Within the extracellular matrix proteoglycans (PG) play a central role in the structural organization and physical functioning of valves and in their capability of settling with endothelial and interstitial cells partially myofibroblasts. We have therefore estimated the effects of decellularization using deoxycholic acid on the structure of the extracellular matrix and PG’s in porcine aortic valves. MATERIAL/METHODS: Cupromeronic blue was used, alone or in combination with OsO(4)/thio-carbo-hydrazide/OsO(4) for electron microscopic visualization. For PG and glycosaminoglycan (GAG) investigation a papain digestion was employed in combination with photometric determination using dimethylmethylene blue. RESULTS: The results indicate that deoxycholic acid affects the compartmentation of the PG-associated interstitial network not significantly. Compared to controls the PG-rich network was preserved even after deoxycholic acid treatment for 48 h. In parallel to electron microscopy immune assays (ELISA) showed smooth muscle cell α-actin to be reduced to 0.96%±0.71 and total soluble protein to 6.68%±2.0 (n=3) of untreated controls. Protein loss corresponded well with the observations in electron micrographs of rupture and efflux of cell content. Further signs of lysis were irregular cell contours and loss of the basement membrane. CONCLUSIONS: Efficient cell-lysis without disintegration or loss of integrity of the interstitial PG network can be achieved by treatment of aortic valves with deoxycholic acid for 48h. This protocol might also be suitable for clinical use to optimize conditions for growth and autologous remodelling of valves.
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spelling pubmed-35607922013-04-24 Extracellular matrix in deoxycholic acid decellularized aortic heart valves Bloch, Oliver Erdbrügger, Wilhelm Völker, Wolfgang Schenk, Alexander Posner, Steffen Konertz, Wolfgang Dohmen, Pascal M. Med Sci Monit Basic Research BACKGROUND: Only limited information is available regarding the influence of decellularization on the extracellular matrix in heart valves. Within the extracellular matrix proteoglycans (PG) play a central role in the structural organization and physical functioning of valves and in their capability of settling with endothelial and interstitial cells partially myofibroblasts. We have therefore estimated the effects of decellularization using deoxycholic acid on the structure of the extracellular matrix and PG’s in porcine aortic valves. MATERIAL/METHODS: Cupromeronic blue was used, alone or in combination with OsO(4)/thio-carbo-hydrazide/OsO(4) for electron microscopic visualization. For PG and glycosaminoglycan (GAG) investigation a papain digestion was employed in combination with photometric determination using dimethylmethylene blue. RESULTS: The results indicate that deoxycholic acid affects the compartmentation of the PG-associated interstitial network not significantly. Compared to controls the PG-rich network was preserved even after deoxycholic acid treatment for 48 h. In parallel to electron microscopy immune assays (ELISA) showed smooth muscle cell α-actin to be reduced to 0.96%±0.71 and total soluble protein to 6.68%±2.0 (n=3) of untreated controls. Protein loss corresponded well with the observations in electron micrographs of rupture and efflux of cell content. Further signs of lysis were irregular cell contours and loss of the basement membrane. CONCLUSIONS: Efficient cell-lysis without disintegration or loss of integrity of the interstitial PG network can be achieved by treatment of aortic valves with deoxycholic acid for 48h. This protocol might also be suitable for clinical use to optimize conditions for growth and autologous remodelling of valves. International Scientific Literature, Inc. 2012-12-04 /pmc/articles/PMC3560792/ /pubmed/23207452 http://dx.doi.org/10.12659/MSM.883618 Text en © Med Sci Monit, 2011 This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.
spellingShingle Basic Research
Bloch, Oliver
Erdbrügger, Wilhelm
Völker, Wolfgang
Schenk, Alexander
Posner, Steffen
Konertz, Wolfgang
Dohmen, Pascal M.
Extracellular matrix in deoxycholic acid decellularized aortic heart valves
title Extracellular matrix in deoxycholic acid decellularized aortic heart valves
title_full Extracellular matrix in deoxycholic acid decellularized aortic heart valves
title_fullStr Extracellular matrix in deoxycholic acid decellularized aortic heart valves
title_full_unstemmed Extracellular matrix in deoxycholic acid decellularized aortic heart valves
title_short Extracellular matrix in deoxycholic acid decellularized aortic heart valves
title_sort extracellular matrix in deoxycholic acid decellularized aortic heart valves
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3560792/
https://www.ncbi.nlm.nih.gov/pubmed/23207452
http://dx.doi.org/10.12659/MSM.883618
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