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Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils

BACKGROUND: Petroleum pollution is a global disaster and there are several soil cleaning methods including bioremediation. METHODS: In a field study, fugal strains were isolated from oil-contaminated sites of Arak refinery (Iran) and their growth ability was checked in potato dextrose agar (PDA) med...

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Autores principales: Mohsenzadeh, Fariba, Chehregani Rad, Abdolkarim, Akbari, Mehrangiz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561093/
https://www.ncbi.nlm.nih.gov/pubmed/23369665
http://dx.doi.org/10.1186/1735-2746-9-26
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author Mohsenzadeh, Fariba
Chehregani Rad, Abdolkarim
Akbari, Mehrangiz
author_facet Mohsenzadeh, Fariba
Chehregani Rad, Abdolkarim
Akbari, Mehrangiz
author_sort Mohsenzadeh, Fariba
collection PubMed
description BACKGROUND: Petroleum pollution is a global disaster and there are several soil cleaning methods including bioremediation. METHODS: In a field study, fugal strains were isolated from oil-contaminated sites of Arak refinery (Iran) and their growth ability was checked in potato dextrose agar (PDA) media containing 0-10% v/v crude oil, the activity of three enzymes (Catalase, Peroxidase and Phenol Oxidase) was evaluated in the fungal colonies and bioremediation ability of the fungi was checked in the experimental pots containing 3 kg sterilized soil and different concentrations of petroleum (0-10% w/w). RESULTS: Four fungal strains, Acromonium sp., Alternaria sp., Aspergillus terreus and Penicillium sp., were selected as the most resistant ones. They were able to growth in the subjected concentrations and Alternaria sp. showed the highest growth ability in the petroleum containing media. The enzyme assay showed that the enzymatic activity was increased in the oil-contaminated media. Bioremediation results showed that the studied fungi were able to decrease petroleum pollution. The highest petroleum removing efficiency of Aspergillus terreus, Penicillium sp., Alternaria sp. and Acromonium sp. was evaluated in the 10%, 8%, 8% and 2% petroleum pollution respectively. CONCLUSIONS: Fungi are important microorganisms in decreasing of petroleum pollution. They have bioremediation potency that is related to their enzymatic activities.
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spelling pubmed-35610932013-02-05 Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils Mohsenzadeh, Fariba Chehregani Rad, Abdolkarim Akbari, Mehrangiz Iranian J Environ Health Sci Eng Research Article BACKGROUND: Petroleum pollution is a global disaster and there are several soil cleaning methods including bioremediation. METHODS: In a field study, fugal strains were isolated from oil-contaminated sites of Arak refinery (Iran) and their growth ability was checked in potato dextrose agar (PDA) media containing 0-10% v/v crude oil, the activity of three enzymes (Catalase, Peroxidase and Phenol Oxidase) was evaluated in the fungal colonies and bioremediation ability of the fungi was checked in the experimental pots containing 3 kg sterilized soil and different concentrations of petroleum (0-10% w/w). RESULTS: Four fungal strains, Acromonium sp., Alternaria sp., Aspergillus terreus and Penicillium sp., were selected as the most resistant ones. They were able to growth in the subjected concentrations and Alternaria sp. showed the highest growth ability in the petroleum containing media. The enzyme assay showed that the enzymatic activity was increased in the oil-contaminated media. Bioremediation results showed that the studied fungi were able to decrease petroleum pollution. The highest petroleum removing efficiency of Aspergillus terreus, Penicillium sp., Alternaria sp. and Acromonium sp. was evaluated in the 10%, 8%, 8% and 2% petroleum pollution respectively. CONCLUSIONS: Fungi are important microorganisms in decreasing of petroleum pollution. They have bioremediation potency that is related to their enzymatic activities. BioMed Central 2012-12-15 /pmc/articles/PMC3561093/ /pubmed/23369665 http://dx.doi.org/10.1186/1735-2746-9-26 Text en Copyright ©2012 Mohsenzadeh et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mohsenzadeh, Fariba
Chehregani Rad, Abdolkarim
Akbari, Mehrangiz
Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils
title Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils
title_full Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils
title_fullStr Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils
title_full_unstemmed Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils
title_short Evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils
title_sort evaluation of oil removal efficiency and enzymatic activity in some fungal strains for bioremediation of petroleum-polluted soils
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561093/
https://www.ncbi.nlm.nih.gov/pubmed/23369665
http://dx.doi.org/10.1186/1735-2746-9-26
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