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Rapid and Non-Enzymatic In Vitro Retrieval of Tumour Cells from Surgical Specimens

The study of tumourigenesis commonly involves the use of established cell lines or single cell suspensions of primary tumours. Standard methods for the generation of short-term tumour cell cultures include the disintegration of tissue based on enzymatic and mechanical stress. Here, we describe a sim...

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Detalles Bibliográficos
Autores principales: Mack, Brigitte, Eggert, Carola, Eder, Katharina, Imrich, Sannia, Baumeister, Philipp, Harréus, Ulrich, Gires, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561176/
https://www.ncbi.nlm.nih.gov/pubmed/23383219
http://dx.doi.org/10.1371/journal.pone.0055540
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author Mack, Brigitte
Eggert, Carola
Eder, Katharina
Imrich, Sannia
Baumeister, Philipp
Harréus, Ulrich
Gires, Olivier
author_facet Mack, Brigitte
Eggert, Carola
Eder, Katharina
Imrich, Sannia
Baumeister, Philipp
Harréus, Ulrich
Gires, Olivier
author_sort Mack, Brigitte
collection PubMed
description The study of tumourigenesis commonly involves the use of established cell lines or single cell suspensions of primary tumours. Standard methods for the generation of short-term tumour cell cultures include the disintegration of tissue based on enzymatic and mechanical stress. Here, we describe a simple and rapid method for the preparation of single cells from primary carcinomas, which is independent of enzymatic treatment and feeder cells. Tumour biopsies are processed to 1 mm(3) cubes termed explants, which are cultured 1–3 days on agarose-coated well plates in specified medium. Through incisions generated in the explants, single cells are retrieved and collected from the culture supernatant and can be used for further analysis including in vitro and in vivo studies. Collected cells retain tumour-forming capacity in xenotransplantation assays, mimic the phenotype of the primary tumour, and facilitate the generation of cell lines.
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spelling pubmed-35611762013-02-04 Rapid and Non-Enzymatic In Vitro Retrieval of Tumour Cells from Surgical Specimens Mack, Brigitte Eggert, Carola Eder, Katharina Imrich, Sannia Baumeister, Philipp Harréus, Ulrich Gires, Olivier PLoS One Research Article The study of tumourigenesis commonly involves the use of established cell lines or single cell suspensions of primary tumours. Standard methods for the generation of short-term tumour cell cultures include the disintegration of tissue based on enzymatic and mechanical stress. Here, we describe a simple and rapid method for the preparation of single cells from primary carcinomas, which is independent of enzymatic treatment and feeder cells. Tumour biopsies are processed to 1 mm(3) cubes termed explants, which are cultured 1–3 days on agarose-coated well plates in specified medium. Through incisions generated in the explants, single cells are retrieved and collected from the culture supernatant and can be used for further analysis including in vitro and in vivo studies. Collected cells retain tumour-forming capacity in xenotransplantation assays, mimic the phenotype of the primary tumour, and facilitate the generation of cell lines. Public Library of Science 2013-01-31 /pmc/articles/PMC3561176/ /pubmed/23383219 http://dx.doi.org/10.1371/journal.pone.0055540 Text en © 2013 Mack et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mack, Brigitte
Eggert, Carola
Eder, Katharina
Imrich, Sannia
Baumeister, Philipp
Harréus, Ulrich
Gires, Olivier
Rapid and Non-Enzymatic In Vitro Retrieval of Tumour Cells from Surgical Specimens
title Rapid and Non-Enzymatic In Vitro Retrieval of Tumour Cells from Surgical Specimens
title_full Rapid and Non-Enzymatic In Vitro Retrieval of Tumour Cells from Surgical Specimens
title_fullStr Rapid and Non-Enzymatic In Vitro Retrieval of Tumour Cells from Surgical Specimens
title_full_unstemmed Rapid and Non-Enzymatic In Vitro Retrieval of Tumour Cells from Surgical Specimens
title_short Rapid and Non-Enzymatic In Vitro Retrieval of Tumour Cells from Surgical Specimens
title_sort rapid and non-enzymatic in vitro retrieval of tumour cells from surgical specimens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561176/
https://www.ncbi.nlm.nih.gov/pubmed/23383219
http://dx.doi.org/10.1371/journal.pone.0055540
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