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Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas

The pancreatic islet is mainly composed of beta-, alpha- and delta-cells with small numbers of pancreatic polypeptide (PP) and epsilon cells. It is known that there is a region in the head of the pancreas that is rich in PP-cells. In the present study, we examined the distribution of PP-cells, and a...

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Autores principales: Wang, Xiaojun, Zielinski, Mark C., Misawa, Ryosuke, Wen, Patrick, Wang, Tian-Yuan, Wang, Cheng-Zhang, Witkowski, Piotr, Hara, Manami
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561199/
https://www.ncbi.nlm.nih.gov/pubmed/23383206
http://dx.doi.org/10.1371/journal.pone.0055501
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author Wang, Xiaojun
Zielinski, Mark C.
Misawa, Ryosuke
Wen, Patrick
Wang, Tian-Yuan
Wang, Cheng-Zhang
Witkowski, Piotr
Hara, Manami
author_facet Wang, Xiaojun
Zielinski, Mark C.
Misawa, Ryosuke
Wen, Patrick
Wang, Tian-Yuan
Wang, Cheng-Zhang
Witkowski, Piotr
Hara, Manami
author_sort Wang, Xiaojun
collection PubMed
description The pancreatic islet is mainly composed of beta-, alpha- and delta-cells with small numbers of pancreatic polypeptide (PP) and epsilon cells. It is known that there is a region in the head of the pancreas that is rich in PP-cells. In the present study, we examined the distribution of PP-cells, and assessed the influence of the PP-cell rich region to quantify the total islet mass. Pancreatic tissues were collected from donors with no history of diabetes or pancreatic diseases (n = 12). A stereological approach with a computer-assisted large-scale analysis of whole pancreatic sections was applied to quantify the entire distribution of endocrine cells within a given section. The initial whole pancreas analysis showed that a PP-cell rich region was largely restricted to the uncinate process with a clear boundary. The distinct distribution of PP-cells includes irregularly shaped clusters composed solely of PP-cells. Furthermore, in the PP-cell rich region, beta- and alpha-cell mass is significantly reduced compared to surrounding PP-cell poor regions. The results suggest that the analysis of the head region should distinguish the PP-cell rich region, which is best examined separately. This study presents an important implication for the regional selection and interpretation of the results.
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spelling pubmed-35611992013-02-04 Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas Wang, Xiaojun Zielinski, Mark C. Misawa, Ryosuke Wen, Patrick Wang, Tian-Yuan Wang, Cheng-Zhang Witkowski, Piotr Hara, Manami PLoS One Research Article The pancreatic islet is mainly composed of beta-, alpha- and delta-cells with small numbers of pancreatic polypeptide (PP) and epsilon cells. It is known that there is a region in the head of the pancreas that is rich in PP-cells. In the present study, we examined the distribution of PP-cells, and assessed the influence of the PP-cell rich region to quantify the total islet mass. Pancreatic tissues were collected from donors with no history of diabetes or pancreatic diseases (n = 12). A stereological approach with a computer-assisted large-scale analysis of whole pancreatic sections was applied to quantify the entire distribution of endocrine cells within a given section. The initial whole pancreas analysis showed that a PP-cell rich region was largely restricted to the uncinate process with a clear boundary. The distinct distribution of PP-cells includes irregularly shaped clusters composed solely of PP-cells. Furthermore, in the PP-cell rich region, beta- and alpha-cell mass is significantly reduced compared to surrounding PP-cell poor regions. The results suggest that the analysis of the head region should distinguish the PP-cell rich region, which is best examined separately. This study presents an important implication for the regional selection and interpretation of the results. Public Library of Science 2013-01-31 /pmc/articles/PMC3561199/ /pubmed/23383206 http://dx.doi.org/10.1371/journal.pone.0055501 Text en © 2013 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Xiaojun
Zielinski, Mark C.
Misawa, Ryosuke
Wen, Patrick
Wang, Tian-Yuan
Wang, Cheng-Zhang
Witkowski, Piotr
Hara, Manami
Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas
title Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas
title_full Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas
title_fullStr Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas
title_full_unstemmed Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas
title_short Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas
title_sort quantitative analysis of pancreatic polypeptide cell distribution in the human pancreas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561199/
https://www.ncbi.nlm.nih.gov/pubmed/23383206
http://dx.doi.org/10.1371/journal.pone.0055501
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