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Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas
The pancreatic islet is mainly composed of beta-, alpha- and delta-cells with small numbers of pancreatic polypeptide (PP) and epsilon cells. It is known that there is a region in the head of the pancreas that is rich in PP-cells. In the present study, we examined the distribution of PP-cells, and a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561199/ https://www.ncbi.nlm.nih.gov/pubmed/23383206 http://dx.doi.org/10.1371/journal.pone.0055501 |
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author | Wang, Xiaojun Zielinski, Mark C. Misawa, Ryosuke Wen, Patrick Wang, Tian-Yuan Wang, Cheng-Zhang Witkowski, Piotr Hara, Manami |
author_facet | Wang, Xiaojun Zielinski, Mark C. Misawa, Ryosuke Wen, Patrick Wang, Tian-Yuan Wang, Cheng-Zhang Witkowski, Piotr Hara, Manami |
author_sort | Wang, Xiaojun |
collection | PubMed |
description | The pancreatic islet is mainly composed of beta-, alpha- and delta-cells with small numbers of pancreatic polypeptide (PP) and epsilon cells. It is known that there is a region in the head of the pancreas that is rich in PP-cells. In the present study, we examined the distribution of PP-cells, and assessed the influence of the PP-cell rich region to quantify the total islet mass. Pancreatic tissues were collected from donors with no history of diabetes or pancreatic diseases (n = 12). A stereological approach with a computer-assisted large-scale analysis of whole pancreatic sections was applied to quantify the entire distribution of endocrine cells within a given section. The initial whole pancreas analysis showed that a PP-cell rich region was largely restricted to the uncinate process with a clear boundary. The distinct distribution of PP-cells includes irregularly shaped clusters composed solely of PP-cells. Furthermore, in the PP-cell rich region, beta- and alpha-cell mass is significantly reduced compared to surrounding PP-cell poor regions. The results suggest that the analysis of the head region should distinguish the PP-cell rich region, which is best examined separately. This study presents an important implication for the regional selection and interpretation of the results. |
format | Online Article Text |
id | pubmed-3561199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35611992013-02-04 Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas Wang, Xiaojun Zielinski, Mark C. Misawa, Ryosuke Wen, Patrick Wang, Tian-Yuan Wang, Cheng-Zhang Witkowski, Piotr Hara, Manami PLoS One Research Article The pancreatic islet is mainly composed of beta-, alpha- and delta-cells with small numbers of pancreatic polypeptide (PP) and epsilon cells. It is known that there is a region in the head of the pancreas that is rich in PP-cells. In the present study, we examined the distribution of PP-cells, and assessed the influence of the PP-cell rich region to quantify the total islet mass. Pancreatic tissues were collected from donors with no history of diabetes or pancreatic diseases (n = 12). A stereological approach with a computer-assisted large-scale analysis of whole pancreatic sections was applied to quantify the entire distribution of endocrine cells within a given section. The initial whole pancreas analysis showed that a PP-cell rich region was largely restricted to the uncinate process with a clear boundary. The distinct distribution of PP-cells includes irregularly shaped clusters composed solely of PP-cells. Furthermore, in the PP-cell rich region, beta- and alpha-cell mass is significantly reduced compared to surrounding PP-cell poor regions. The results suggest that the analysis of the head region should distinguish the PP-cell rich region, which is best examined separately. This study presents an important implication for the regional selection and interpretation of the results. Public Library of Science 2013-01-31 /pmc/articles/PMC3561199/ /pubmed/23383206 http://dx.doi.org/10.1371/journal.pone.0055501 Text en © 2013 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Xiaojun Zielinski, Mark C. Misawa, Ryosuke Wen, Patrick Wang, Tian-Yuan Wang, Cheng-Zhang Witkowski, Piotr Hara, Manami Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas |
title | Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas |
title_full | Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas |
title_fullStr | Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas |
title_full_unstemmed | Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas |
title_short | Quantitative Analysis of Pancreatic Polypeptide Cell Distribution in the Human Pancreas |
title_sort | quantitative analysis of pancreatic polypeptide cell distribution in the human pancreas |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561199/ https://www.ncbi.nlm.nih.gov/pubmed/23383206 http://dx.doi.org/10.1371/journal.pone.0055501 |
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