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Reduced Interleukin-4 Receptor α Expression on CD8(+) T Cells Correlates with Higher Quality Anti-Viral Immunity
With the hope of understanding how interleukin (IL)-4 and IL-13 modulated quality of anti-viral CD8(+) T cells, we evaluated the expression of receptors for these cytokines following a range of viral infections (e.g. pox viruses and influenza virus). Results clearly indicated that unlike other IL-4/...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561338/ https://www.ncbi.nlm.nih.gov/pubmed/23383283 http://dx.doi.org/10.1371/journal.pone.0055788 |
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author | Wijesundara, Danushka K. Tscharke, David C. Jackson, Ronald J. Ranasinghe, Charani |
author_facet | Wijesundara, Danushka K. Tscharke, David C. Jackson, Ronald J. Ranasinghe, Charani |
author_sort | Wijesundara, Danushka K. |
collection | PubMed |
description | With the hope of understanding how interleukin (IL)-4 and IL-13 modulated quality of anti-viral CD8(+) T cells, we evaluated the expression of receptors for these cytokines following a range of viral infections (e.g. pox viruses and influenza virus). Results clearly indicated that unlike other IL-4/IL-13 receptor subunits, IL-4 receptor α (IL-4Rα) was significantly down-regulated on anti-viral CD8(+) T cells in a cognate antigen dependent manner. The infection of gene knockout mice and wild-type (WT) mice with vaccinia virus (VV) or VV expressing IL-4 confirmed that IL-4, IL-13 and signal transducer and activator of transcription 6 (STAT6) were required to increase IL-4Rα expression on CD8(+) T cells, but not interferon (IFN)-γ. STAT6 dependent elevation of IL-4Rα expression on CD8(+) T cells was a feature of poor quality anti-viral CD8(+) T cell immunity as measured by the production of IFN-γ and tumor necrosis factor α (TNF-α) in response to VV antigen stimulation in vitro. We propose that down-regulation of IL-4Rα, but not the other IL-4/IL-13 receptor subunits, is a mechanism by which CD8(+) T cells reduce responsiveness to IL-4 and IL-13. This can improve the quality of anti-viral CD8(+) T cell immunity. Our findings have important implications in understanding anti-viral CD8(+) T cell immunity and designing effective vaccines against chronic viral infections. |
format | Online Article Text |
id | pubmed-3561338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35613382013-02-04 Reduced Interleukin-4 Receptor α Expression on CD8(+) T Cells Correlates with Higher Quality Anti-Viral Immunity Wijesundara, Danushka K. Tscharke, David C. Jackson, Ronald J. Ranasinghe, Charani PLoS One Research Article With the hope of understanding how interleukin (IL)-4 and IL-13 modulated quality of anti-viral CD8(+) T cells, we evaluated the expression of receptors for these cytokines following a range of viral infections (e.g. pox viruses and influenza virus). Results clearly indicated that unlike other IL-4/IL-13 receptor subunits, IL-4 receptor α (IL-4Rα) was significantly down-regulated on anti-viral CD8(+) T cells in a cognate antigen dependent manner. The infection of gene knockout mice and wild-type (WT) mice with vaccinia virus (VV) or VV expressing IL-4 confirmed that IL-4, IL-13 and signal transducer and activator of transcription 6 (STAT6) were required to increase IL-4Rα expression on CD8(+) T cells, but not interferon (IFN)-γ. STAT6 dependent elevation of IL-4Rα expression on CD8(+) T cells was a feature of poor quality anti-viral CD8(+) T cell immunity as measured by the production of IFN-γ and tumor necrosis factor α (TNF-α) in response to VV antigen stimulation in vitro. We propose that down-regulation of IL-4Rα, but not the other IL-4/IL-13 receptor subunits, is a mechanism by which CD8(+) T cells reduce responsiveness to IL-4 and IL-13. This can improve the quality of anti-viral CD8(+) T cell immunity. Our findings have important implications in understanding anti-viral CD8(+) T cell immunity and designing effective vaccines against chronic viral infections. Public Library of Science 2013-01-31 /pmc/articles/PMC3561338/ /pubmed/23383283 http://dx.doi.org/10.1371/journal.pone.0055788 Text en © 2013 Wijesundara et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wijesundara, Danushka K. Tscharke, David C. Jackson, Ronald J. Ranasinghe, Charani Reduced Interleukin-4 Receptor α Expression on CD8(+) T Cells Correlates with Higher Quality Anti-Viral Immunity |
title | Reduced Interleukin-4 Receptor α Expression on CD8(+) T Cells Correlates with Higher Quality Anti-Viral Immunity |
title_full | Reduced Interleukin-4 Receptor α Expression on CD8(+) T Cells Correlates with Higher Quality Anti-Viral Immunity |
title_fullStr | Reduced Interleukin-4 Receptor α Expression on CD8(+) T Cells Correlates with Higher Quality Anti-Viral Immunity |
title_full_unstemmed | Reduced Interleukin-4 Receptor α Expression on CD8(+) T Cells Correlates with Higher Quality Anti-Viral Immunity |
title_short | Reduced Interleukin-4 Receptor α Expression on CD8(+) T Cells Correlates with Higher Quality Anti-Viral Immunity |
title_sort | reduced interleukin-4 receptor α expression on cd8(+) t cells correlates with higher quality anti-viral immunity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561338/ https://www.ncbi.nlm.nih.gov/pubmed/23383283 http://dx.doi.org/10.1371/journal.pone.0055788 |
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