Ultra-bright Photoactivatable Fluorophores Created by Reductive Caging

Sub-diffraction-limit imaging can be achieved by sequential localization of photoactivatable fluorophores, where the image resolution depends on the number of photons detected per localization. Here, we report a strategy for fluorophore caging that creates photoactivatable probes with high photon yi...

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Detalles Bibliográficos
Autores principales: Vaughan, Joshua C., Jia, Shu, Zhuang, Xiaowei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561463/
https://www.ncbi.nlm.nih.gov/pubmed/23103881
http://dx.doi.org/10.1038/nmeth.2214
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author Vaughan, Joshua C.
Jia, Shu
Zhuang, Xiaowei
author_facet Vaughan, Joshua C.
Jia, Shu
Zhuang, Xiaowei
author_sort Vaughan, Joshua C.
collection PubMed
description Sub-diffraction-limit imaging can be achieved by sequential localization of photoactivatable fluorophores, where the image resolution depends on the number of photons detected per localization. Here, we report a strategy for fluorophore caging that creates photoactivatable probes with high photon yields. Upon photoactivation, these probes can provide 10(4)–10(6) photons per localization and allow imaging of fixed samples with resolutions of several nanometers. This strategy can be applied to many fluorophores across the visible spectrum.
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spelling pubmed-35614632013-06-01 Ultra-bright Photoactivatable Fluorophores Created by Reductive Caging Vaughan, Joshua C. Jia, Shu Zhuang, Xiaowei Nat Methods Article Sub-diffraction-limit imaging can be achieved by sequential localization of photoactivatable fluorophores, where the image resolution depends on the number of photons detected per localization. Here, we report a strategy for fluorophore caging that creates photoactivatable probes with high photon yields. Upon photoactivation, these probes can provide 10(4)–10(6) photons per localization and allow imaging of fixed samples with resolutions of several nanometers. This strategy can be applied to many fluorophores across the visible spectrum. 2012-10-28 2012-12 /pmc/articles/PMC3561463/ /pubmed/23103881 http://dx.doi.org/10.1038/nmeth.2214 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Vaughan, Joshua C.
Jia, Shu
Zhuang, Xiaowei
Ultra-bright Photoactivatable Fluorophores Created by Reductive Caging
title Ultra-bright Photoactivatable Fluorophores Created by Reductive Caging
title_full Ultra-bright Photoactivatable Fluorophores Created by Reductive Caging
title_fullStr Ultra-bright Photoactivatable Fluorophores Created by Reductive Caging
title_full_unstemmed Ultra-bright Photoactivatable Fluorophores Created by Reductive Caging
title_short Ultra-bright Photoactivatable Fluorophores Created by Reductive Caging
title_sort ultra-bright photoactivatable fluorophores created by reductive caging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561463/
https://www.ncbi.nlm.nih.gov/pubmed/23103881
http://dx.doi.org/10.1038/nmeth.2214
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AT zhuangxiaowei ultrabrightphotoactivatablefluorophorescreatedbyreductivecaging

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