PUB-NChIP—“in vivo biotinylation” approach to study chromatin in proximity to a protein of interest

We have developed an approach termed PUB-NChIP (proximity utilizing biotinylation with native ChIP) to purify and study the protein composition of chromatin in proximity to a nuclear protein of interest. It is based on coexpression of (1) a protein of interest, fused with the bacterial biotin ligase...

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Detalles Bibliográficos
Autores principales: Shoaib, Muhammad, Kulyyassov, Arman, Robin, Chloé, Winczura, Kinga, Tarlykov, Pavel, Despas, Emmanuelle, Kannouche, Patricia, Ramanculov, Erlan, Lipinski, Marc, Ogryzko, Vasily
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2013
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561874/
https://www.ncbi.nlm.nih.gov/pubmed/23038767
http://dx.doi.org/10.1101/gr.134874.111

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