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Absence of a universal element for tRNA(His) identity in Acanthamoeba castellanii

The additional G(−1) nucleotide on tRNA(His) is a nearly universal feature that specifies tRNA(His) identity in all three domains of life. In eukaryotes, the G(−1) identity element is obtained by a post-transcriptional pathway, through the unusual 3′–5′ polymerase activity of the highly conserved tR...

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Autores principales: Rao, Bhalchandra S., Mohammad, Fuad, Gray, Michael W., Jackman, Jane E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561963/
https://www.ncbi.nlm.nih.gov/pubmed/23241387
http://dx.doi.org/10.1093/nar/gks1242
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author Rao, Bhalchandra S.
Mohammad, Fuad
Gray, Michael W.
Jackman, Jane E.
author_facet Rao, Bhalchandra S.
Mohammad, Fuad
Gray, Michael W.
Jackman, Jane E.
author_sort Rao, Bhalchandra S.
collection PubMed
description The additional G(−1) nucleotide on tRNA(His) is a nearly universal feature that specifies tRNA(His) identity in all three domains of life. In eukaryotes, the G(−1) identity element is obtained by a post-transcriptional pathway, through the unusual 3′–5′ polymerase activity of the highly conserved tRNA(His) guanylyltransferase (Thg1) enzyme, and no examples of eukaryotic histidyl-tRNAs that lack this essential element have been identified. Here we report that the eukaryote Acanthamoeba castellanii lacks the G(−1) identity element on its tRNA(His), consistent with the lack of a gene encoding a bona fide Thg1 ortholog in the A. castellanii genome. Moreover, the cytosolic histidyl-tRNA synthetase in A. castellanii exhibits an unusual tRNA substrate specificity, efficiently aminoacylating tRNA(His) regardless of the presence of G(−1). A. castellanii does contain two Thg1-related genes (encoding Thg1-like proteins, TLPs), but the biochemical properties we associate here with these proteins are consistent with a function for these TLPs in separate pathways unrelated to tRNA(His) metabolism, such as mitochondrial tRNA repair during 5′-editing.
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spelling pubmed-35619632013-02-01 Absence of a universal element for tRNA(His) identity in Acanthamoeba castellanii Rao, Bhalchandra S. Mohammad, Fuad Gray, Michael W. Jackman, Jane E. Nucleic Acids Res RNA The additional G(−1) nucleotide on tRNA(His) is a nearly universal feature that specifies tRNA(His) identity in all three domains of life. In eukaryotes, the G(−1) identity element is obtained by a post-transcriptional pathway, through the unusual 3′–5′ polymerase activity of the highly conserved tRNA(His) guanylyltransferase (Thg1) enzyme, and no examples of eukaryotic histidyl-tRNAs that lack this essential element have been identified. Here we report that the eukaryote Acanthamoeba castellanii lacks the G(−1) identity element on its tRNA(His), consistent with the lack of a gene encoding a bona fide Thg1 ortholog in the A. castellanii genome. Moreover, the cytosolic histidyl-tRNA synthetase in A. castellanii exhibits an unusual tRNA substrate specificity, efficiently aminoacylating tRNA(His) regardless of the presence of G(−1). A. castellanii does contain two Thg1-related genes (encoding Thg1-like proteins, TLPs), but the biochemical properties we associate here with these proteins are consistent with a function for these TLPs in separate pathways unrelated to tRNA(His) metabolism, such as mitochondrial tRNA repair during 5′-editing. Oxford University Press 2013-02 2012-12-14 /pmc/articles/PMC3561963/ /pubmed/23241387 http://dx.doi.org/10.1093/nar/gks1242 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle RNA
Rao, Bhalchandra S.
Mohammad, Fuad
Gray, Michael W.
Jackman, Jane E.
Absence of a universal element for tRNA(His) identity in Acanthamoeba castellanii
title Absence of a universal element for tRNA(His) identity in Acanthamoeba castellanii
title_full Absence of a universal element for tRNA(His) identity in Acanthamoeba castellanii
title_fullStr Absence of a universal element for tRNA(His) identity in Acanthamoeba castellanii
title_full_unstemmed Absence of a universal element for tRNA(His) identity in Acanthamoeba castellanii
title_short Absence of a universal element for tRNA(His) identity in Acanthamoeba castellanii
title_sort absence of a universal element for trna(his) identity in acanthamoeba castellanii
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561963/
https://www.ncbi.nlm.nih.gov/pubmed/23241387
http://dx.doi.org/10.1093/nar/gks1242
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