Cargando…

Systematic screens of proteins binding to synthetic microRNA precursors

We describe a new, broadly applicable methodology for screening in parallel interactions of RNA-binding proteins (RBPs) with large numbers of microRNA (miRNA) precursors and for determining their affinities in native form in the presence of cellular factors. The assays aim at identifying pre-miRNAs...

Descripción completa

Detalles Bibliográficos
Autores principales:	Towbin, Harry, Wenter, Philipp, Guennewig, Boris, Imig, Jochen, Zagalak, Julian A., Gerber, André P., Hall, Jonathan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2013
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561989/ https://www.ncbi.nlm.nih.gov/pubmed/23221640 http://dx.doi.org/10.1093/nar/gks1197

Ejemplares similares

Short, synthetic and selectively (13)C-labeled RNA sequences for the NMR structure determination of protein–RNA complexes
por: Wenter, Philipp, et al.
Publicado: (2006)

Synthetic pre-microRNAs reveal dual-strand activity of miR-34a on TNF-α
por: Guennewig, Boris, et al.
Publicado: (2014)

Properties of short double-stranded RNAs carrying randomized base pairs: toward better controls for RNAi experiments
por: Zagalak, Julian A., et al.
Publicado: (2015)

CLIP-based prediction of mammalian microRNA binding sites
por: Liu, Chaochun, et al.
Publicado: (2013)

Modeling the combined effect of RNA-binding proteins and microRNAs in post-transcriptional regulation
por: HafezQorani, Saber, et al.
Publicado: (2016)

Systemic CLIP-seq analysis and game theory approach to model microRNA mode of binding
por: Serra, Fabrizio, et al.
Publicado: (2021)

A single anti-microRNA antisense oligodeoxyribonucleotide (AMO) targeting multiple microRNAs offers an improved approach for microRNA interference
por: Lu, Yanjie, et al.
Publicado: (2009)

The microRNA body map: dissecting microRNA function through integrative genomics
por: Mestdagh, Pieter, et al.
Publicado: (2011)

Prioritizing human cancer microRNAs based on genes’ functional consistency between microRNA and cancer
por: Li, Xia, et al.
Publicado: (2011)

Integrated analysis of microRNA and mRNA expression: adding biological significance to microRNA target predictions
por: van Iterson, Maarten, et al.
Publicado: (2013)

Short loop-targeting oligoribonucleotides antagonize Lin28 and enable pre-let-7 processing and suppression of cell growth in let-7-deficient cancer cells
por: Roos, Martina, et al.
Publicado: (2015)

Identification of microRNAs with regulatory potential using a matched microRNA-mRNA time-course data
por: Jayaswal, Vivek, et al.
Publicado: (2009)

Amplified microRNA detection by templated chemistry
por: Harcourt, Emily M., et al.
Publicado: (2012)

Silencing microRNA by interfering nanoparticles in mice
por: Su, Jie, et al.
Publicado: (2011)

Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs
por: Hsu, Ren-Jun, et al.
Publicado: (2009)

Inferring causative variants in microRNA target sites
por: Thomas, Laurent F., et al.
Publicado: (2011)

Towards computational prediction of microRNA function and activity
por: Ulitsky, Igor, et al.
Publicado: (2010)

A structural view of microRNA–target recognition
por: Leoni, Guido, et al.
Publicado: (2016)

A novel microarray approach reveals new tissue-specific signatures of known and predicted mammalian microRNAs
por: Beuvink, Iwan, et al.
Publicado: (2007)

Quality Assessment and Data Analysis for microRNA Expression Arrays
por: Sarkar, D., et al.
Publicado: (2009)

Imaging individual microRNAs in single mammalian cells in situ
por: Lu, Jing, et al.
Publicado: (2009)

MicroRNA enrichment among short ‘ultraconserved’ sequences in insects
por: Tran, T., et al.
Publicado: (2006)

In vitro quantification of specific microRNA using molecular beacons
por: Baker, Meredith B., et al.
Publicado: (2012)

A functional assay for microRNA target identification and validation
por: Gäken, Joop, et al.
Publicado: (2012)

Deciphering the transcriptional regulation of microRNA genes in humans with ACTLocater
por: Xiao, Zhen-Dong, et al.
Publicado: (2013)

Large-scale profiling of microRNAs for The Cancer Genome Atlas
por: Chu, Andy, et al.
Publicado: (2016)

A conformation-induced fluorescence method for microRNA detection
por: Aw, Sherry S., et al.
Publicado: (2016)

The multiMiR R package and database: integration of microRNA–target interactions along with their disease and drug associations
por: Ru, Yuanbin, et al.
Publicado: (2014)

A human microRNA precursor binding to folic acid discovered by small RNA transcriptomic SELEX
por: Terasaka, Naohiro, et al.
Publicado: (2016)

MicroRNA expression profiling of single whole embryonic stem cells
por: Tang, Fuchou, et al.
Publicado: (2006)

Real-time quantification of microRNAs by stem–loop RT–PCR
por: Chen, Caifu, et al.
Publicado: (2005)

Inducible microRNA expression by an all-in-one episomal vector system
por: Epanchintsev, Alexey, et al.
Publicado: (2006)

Blocking of targeted microRNAs from next-generation sequencing libraries
por: Roberts, Brian S., et al.
Publicado: (2015)

A framework for improving microRNA prediction in non-human genomes
por: Peace, Robert J., et al.
Publicado: (2015)

Digital direct detection of microRNAs using single molecule arrays
por: Cohen, Limor, et al.
Publicado: (2017)

Mouse microRNA profiles determined with a new and sensitive cloning method
por: Takada, Shuji, et al.
Publicado: (2006)

Analysis of microRNA signatures using size-coded ligation-mediated PCR
por: Arefian, Ehsan, et al.
Publicado: (2011)

Isoform-level microRNA-155 target prediction using RNA-seq
por: Deng, Nan, et al.
Publicado: (2011)

Detecting and characterizing microRNAs of diverse genomic origins via miRvial
por: Xia, Jing, et al.
Publicado: (2017)

Highly sensitive and specific microRNA expression profiling using BeadArray technology
por: Chen, Jing, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_t6ggrmvgq2gn83777ki03khmfj