A Reliable Way to Detect Endogenous Murine β-Amyloid

Unraveling the normal physiologic role of β-amyloid is likely crucial to understanding the pathogenesis of Alzheimer's disease. However, progress on this question is currently limited by the high background of many ELISAs for murine β-amyloid. Here, we examine the background signal of several m...

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Detalles Bibliográficos
Autores principales: Teich, Andrew F., Patel, Mitesh, Arancio, Ottavio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562188/
https://www.ncbi.nlm.nih.gov/pubmed/23383341
http://dx.doi.org/10.1371/journal.pone.0055647
Descripción
Sumario:Unraveling the normal physiologic role of β-amyloid is likely crucial to understanding the pathogenesis of Alzheimer's disease. However, progress on this question is currently limited by the high background of many ELISAs for murine β-amyloid. Here, we examine the background signal of several murine β-amyloid ELISAs, and conclude that the majority of the background is from non-APP derived proteins. Most importantly, we identify ELISAs that eliminate this background signal.

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