A Reliable Way to Detect Endogenous Murine β-Amyloid

Unraveling the normal physiologic role of β-amyloid is likely crucial to understanding the pathogenesis of Alzheimer's disease. However, progress on this question is currently limited by the high background of many ELISAs for murine β-amyloid. Here, we examine the background signal of several m...

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Detalles Bibliográficos
Autores principales: Teich, Andrew F., Patel, Mitesh, Arancio, Ottavio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562188/
https://www.ncbi.nlm.nih.gov/pubmed/23383341
http://dx.doi.org/10.1371/journal.pone.0055647
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author Teich, Andrew F.
Patel, Mitesh
Arancio, Ottavio
author_facet Teich, Andrew F.
Patel, Mitesh
Arancio, Ottavio
author_sort Teich, Andrew F.
collection PubMed
description Unraveling the normal physiologic role of β-amyloid is likely crucial to understanding the pathogenesis of Alzheimer's disease. However, progress on this question is currently limited by the high background of many ELISAs for murine β-amyloid. Here, we examine the background signal of several murine β-amyloid ELISAs, and conclude that the majority of the background is from non-APP derived proteins. Most importantly, we identify ELISAs that eliminate this background signal.
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spelling pubmed-35621882013-02-04 A Reliable Way to Detect Endogenous Murine β-Amyloid Teich, Andrew F. Patel, Mitesh Arancio, Ottavio PLoS One Research Article Unraveling the normal physiologic role of β-amyloid is likely crucial to understanding the pathogenesis of Alzheimer's disease. However, progress on this question is currently limited by the high background of many ELISAs for murine β-amyloid. Here, we examine the background signal of several murine β-amyloid ELISAs, and conclude that the majority of the background is from non-APP derived proteins. Most importantly, we identify ELISAs that eliminate this background signal. Public Library of Science 2013-02-01 /pmc/articles/PMC3562188/ /pubmed/23383341 http://dx.doi.org/10.1371/journal.pone.0055647 Text en © 2013 Teich et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Teich, Andrew F.
Patel, Mitesh
Arancio, Ottavio
A Reliable Way to Detect Endogenous Murine β-Amyloid
title A Reliable Way to Detect Endogenous Murine β-Amyloid
title_full A Reliable Way to Detect Endogenous Murine β-Amyloid
title_fullStr A Reliable Way to Detect Endogenous Murine β-Amyloid
title_full_unstemmed A Reliable Way to Detect Endogenous Murine β-Amyloid
title_short A Reliable Way to Detect Endogenous Murine β-Amyloid
title_sort reliable way to detect endogenous murine β-amyloid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562188/
https://www.ncbi.nlm.nih.gov/pubmed/23383341
http://dx.doi.org/10.1371/journal.pone.0055647
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