In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction

L-amino acid oxidase (LAAO) is attracting increasing attention due to its important functions. Diverse detection methods with their own properties have been developed for characterization of LAAO. In the present study, a simple, rapid, sensitive, cost-effective and reproducible method for quantitati...

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Detalles Bibliográficos
Autores principales: Yu, Zhiliang, Zhou, Ning, Zhao, Chuntian, Qiu, Juanping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562322/
https://www.ncbi.nlm.nih.gov/pubmed/23383337
http://dx.doi.org/10.1371/journal.pone.0055548
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author Yu, Zhiliang
Zhou, Ning
Zhao, Chuntian
Qiu, Juanping
author_facet Yu, Zhiliang
Zhou, Ning
Zhao, Chuntian
Qiu, Juanping
author_sort Yu, Zhiliang
collection PubMed
description L-amino acid oxidase (LAAO) is attracting increasing attention due to its important functions. Diverse detection methods with their own properties have been developed for characterization of LAAO. In the present study, a simple, rapid, sensitive, cost-effective and reproducible method for quantitative in-gel determination of LAAO activity based on the visualization of Prussian blue-forming reaction is described. Coupled with SDS-PAGE, this Prussian blue agar assay can be directly used to determine the numbers and approximate molecular weights of LAAO in one step, allowing straightforward application for purification and sequence identification of LAAO from diverse samples.
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spelling pubmed-35623222013-02-04 In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction Yu, Zhiliang Zhou, Ning Zhao, Chuntian Qiu, Juanping PLoS One Research Article L-amino acid oxidase (LAAO) is attracting increasing attention due to its important functions. Diverse detection methods with their own properties have been developed for characterization of LAAO. In the present study, a simple, rapid, sensitive, cost-effective and reproducible method for quantitative in-gel determination of LAAO activity based on the visualization of Prussian blue-forming reaction is described. Coupled with SDS-PAGE, this Prussian blue agar assay can be directly used to determine the numbers and approximate molecular weights of LAAO in one step, allowing straightforward application for purification and sequence identification of LAAO from diverse samples. Public Library of Science 2013-02-01 /pmc/articles/PMC3562322/ /pubmed/23383337 http://dx.doi.org/10.1371/journal.pone.0055548 Text en © 2013 Yu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yu, Zhiliang
Zhou, Ning
Zhao, Chuntian
Qiu, Juanping
In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction
title In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction
title_full In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction
title_fullStr In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction
title_full_unstemmed In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction
title_short In-Gel Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Prussian Blue-Forming Reaction
title_sort in-gel determination of l-amino acid oxidase activity based on the visualization of prussian blue-forming reaction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562322/
https://www.ncbi.nlm.nih.gov/pubmed/23383337
http://dx.doi.org/10.1371/journal.pone.0055548
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