Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells

The stochastic nature of generating eukaryotic transcripts challenges conventional methods for obtaining and analyzing single-cell gene expression data. In order to address the inherent noise, detailed methods are described on how to collect data on multiple genes in a large number of single cells u...

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Detalles Bibliográficos
Autores principales: Livak, Kenneth J., Wills, Quin F., Tipping, Alex J., Datta, Krishnalekha, Mittal, Rowena, Goldson, Andrew J., Sexton, Darren W., Holmes, Chris C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562442/
https://www.ncbi.nlm.nih.gov/pubmed/23079396
http://dx.doi.org/10.1016/j.ymeth.2012.10.004

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562442/
https://www.ncbi.nlm.nih.gov/pubmed/23079396
http://dx.doi.org/10.1016/j.ymeth.2012.10.004

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