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Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells
The stochastic nature of generating eukaryotic transcripts challenges conventional methods for obtaining and analyzing single-cell gene expression data. In order to address the inherent noise, detailed methods are described on how to collect data on multiple genes in a large number of single cells u...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Academic Press
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562442/ https://www.ncbi.nlm.nih.gov/pubmed/23079396 http://dx.doi.org/10.1016/j.ymeth.2012.10.004 |