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Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control

Apical extracellular matrix filling the lumen controls the morphology and geometry of epithelial tubes during development, yet the regulation of luminal protein composition and its role in tube morphogenesis are not well understood. Here we show that an endosomal-retrieval machinery consisting of Ra...

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Autores principales: Dong, Bo, Kakihara, Ken, Otani, Tetsuhisa, Wada, Housei, Hayashi, Shigeo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562448/
https://www.ncbi.nlm.nih.gov/pubmed/23322046
http://dx.doi.org/10.1038/ncomms2347
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author Dong, Bo
Kakihara, Ken
Otani, Tetsuhisa
Wada, Housei
Hayashi, Shigeo
author_facet Dong, Bo
Kakihara, Ken
Otani, Tetsuhisa
Wada, Housei
Hayashi, Shigeo
author_sort Dong, Bo
collection PubMed
description Apical extracellular matrix filling the lumen controls the morphology and geometry of epithelial tubes during development, yet the regulation of luminal protein composition and its role in tube morphogenesis are not well understood. Here we show that an endosomal-retrieval machinery consisting of Rab9, retromer and actin nucleator WASH (Wiskott–Aldrich Syndrome Protein and SCAR Homolog) regulates selective recycling of the luminal protein Serpentine in the Drosophila trachea. Secreted Serpentine is endocytosed and sorted into the late endosome. Vps35, WASH and actin filaments differentially localize at the Rab9-enriched subdomains of the endosomal membrane, where Serpentine containing vesicles bud off. In Rab9, Vps35 and WASH mutants, Serpentine was secreted normally into the tracheal lumen, but the luminal quantities were depleted at later stages, resulting in excessively elongated tubes. In contrast, secretion of many luminal proteins was unaffected, suggesting that retrograde trafficking of a specific class of luminal proteins is a pivotal rate-limiting mechanism for continuous tube length regulation.
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spelling pubmed-35624482013-02-04 Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control Dong, Bo Kakihara, Ken Otani, Tetsuhisa Wada, Housei Hayashi, Shigeo Nat Commun Article Apical extracellular matrix filling the lumen controls the morphology and geometry of epithelial tubes during development, yet the regulation of luminal protein composition and its role in tube morphogenesis are not well understood. Here we show that an endosomal-retrieval machinery consisting of Rab9, retromer and actin nucleator WASH (Wiskott–Aldrich Syndrome Protein and SCAR Homolog) regulates selective recycling of the luminal protein Serpentine in the Drosophila trachea. Secreted Serpentine is endocytosed and sorted into the late endosome. Vps35, WASH and actin filaments differentially localize at the Rab9-enriched subdomains of the endosomal membrane, where Serpentine containing vesicles bud off. In Rab9, Vps35 and WASH mutants, Serpentine was secreted normally into the tracheal lumen, but the luminal quantities were depleted at later stages, resulting in excessively elongated tubes. In contrast, secretion of many luminal proteins was unaffected, suggesting that retrograde trafficking of a specific class of luminal proteins is a pivotal rate-limiting mechanism for continuous tube length regulation. Nature Pub. Group 2013-01-15 /pmc/articles/PMC3562448/ /pubmed/23322046 http://dx.doi.org/10.1038/ncomms2347 Text en Copyright © 2013, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Dong, Bo
Kakihara, Ken
Otani, Tetsuhisa
Wada, Housei
Hayashi, Shigeo
Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control
title Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control
title_full Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control
title_fullStr Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control
title_full_unstemmed Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control
title_short Rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control
title_sort rab9 and retromer regulate retrograde trafficking of luminal protein required for epithelial tube length control
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562448/
https://www.ncbi.nlm.nih.gov/pubmed/23322046
http://dx.doi.org/10.1038/ncomms2347
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