Cargando…
Transgenerational gene silencing causes gain of virulence in a plant pathogen
Avirulence (Avr) genes of plant pathogens encode effector proteins that trigger immunity in plants carrying appropriate resistance (R) genes. The Phytophthora sojae Avr3a gene displays allelic variation in messenger RNA transcript levels. P. sojae strains with detectable Avr3a gene transcripts are a...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562452/ https://www.ncbi.nlm.nih.gov/pubmed/23322037 http://dx.doi.org/10.1038/ncomms2354 |
_version_ | 1782258087721172992 |
---|---|
author | Qutob, Dinah Patrick Chapman, B. Gijzen, Mark |
author_facet | Qutob, Dinah Patrick Chapman, B. Gijzen, Mark |
author_sort | Qutob, Dinah |
collection | PubMed |
description | Avirulence (Avr) genes of plant pathogens encode effector proteins that trigger immunity in plants carrying appropriate resistance (R) genes. The Phytophthora sojae Avr3a gene displays allelic variation in messenger RNA transcript levels. P. sojae strains with detectable Avr3a gene transcripts are avirulent on plants carrying the R-gene Rps3a, whereas strains lacking Avr3a mRNA escape detection by Rps3a and are virulent. Here we show non-Mendelian interactions between naturally occurring Avr3a alleles that result in transgenerational gene silencing, and we identify small RNA molecules of 25 nucleotides that are abundant in gene-silenced strains but not in strains with Avr3a mRNA. This example of transgenerational gene silencing is exceptional because it is naturally occurring and results in gain of virulence in a pathogenic organism. |
format | Online Article Text |
id | pubmed-3562452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-35624522013-02-04 Transgenerational gene silencing causes gain of virulence in a plant pathogen Qutob, Dinah Patrick Chapman, B. Gijzen, Mark Nat Commun Article Avirulence (Avr) genes of plant pathogens encode effector proteins that trigger immunity in plants carrying appropriate resistance (R) genes. The Phytophthora sojae Avr3a gene displays allelic variation in messenger RNA transcript levels. P. sojae strains with detectable Avr3a gene transcripts are avirulent on plants carrying the R-gene Rps3a, whereas strains lacking Avr3a mRNA escape detection by Rps3a and are virulent. Here we show non-Mendelian interactions between naturally occurring Avr3a alleles that result in transgenerational gene silencing, and we identify small RNA molecules of 25 nucleotides that are abundant in gene-silenced strains but not in strains with Avr3a mRNA. This example of transgenerational gene silencing is exceptional because it is naturally occurring and results in gain of virulence in a pathogenic organism. Nature Pub. Group 2013-01-15 /pmc/articles/PMC3562452/ /pubmed/23322037 http://dx.doi.org/10.1038/ncomms2354 Text en Copyright © 2013, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
spellingShingle | Article Qutob, Dinah Patrick Chapman, B. Gijzen, Mark Transgenerational gene silencing causes gain of virulence in a plant pathogen |
title | Transgenerational gene silencing causes gain of virulence in a plant pathogen |
title_full | Transgenerational gene silencing causes gain of virulence in a plant pathogen |
title_fullStr | Transgenerational gene silencing causes gain of virulence in a plant pathogen |
title_full_unstemmed | Transgenerational gene silencing causes gain of virulence in a plant pathogen |
title_short | Transgenerational gene silencing causes gain of virulence in a plant pathogen |
title_sort | transgenerational gene silencing causes gain of virulence in a plant pathogen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3562452/ https://www.ncbi.nlm.nih.gov/pubmed/23322037 http://dx.doi.org/10.1038/ncomms2354 |
work_keys_str_mv | AT qutobdinah transgenerationalgenesilencingcausesgainofvirulenceinaplantpathogen AT patrickchapmanb transgenerationalgenesilencingcausesgainofvirulenceinaplantpathogen AT gijzenmark transgenerationalgenesilencingcausesgainofvirulenceinaplantpathogen |