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Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy
Acute schistosomiasis is associated with a primary exposure and is more commonly seen in nonimmune individuals traveling through endemic regions. In this study, we have focused on the cytokine profile of T lymphocytes evaluated in circulating leukocytes of acute Schistosomiasis mansoni-infected pati...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563181/ https://www.ncbi.nlm.nih.gov/pubmed/23401741 http://dx.doi.org/10.1155/2013/909134 |
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author | Silveira-Lemos, Denise Fernandes Costa-Silva, Matheus Cardoso de Oliveira Silveira, Amanda Azevedo Batista, Mauricio Alves Oliveira-Fraga, Lúcia Soares Silveira, Alda Maria Barbosa Alvarez, Maria Carolina Martins-Filho, Olindo Assis Gazzinelli, Giovanni Corrêa-Oliveira, Rodrigo Teixeira-Carvalho, Andréa |
author_facet | Silveira-Lemos, Denise Fernandes Costa-Silva, Matheus Cardoso de Oliveira Silveira, Amanda Azevedo Batista, Mauricio Alves Oliveira-Fraga, Lúcia Soares Silveira, Alda Maria Barbosa Alvarez, Maria Carolina Martins-Filho, Olindo Assis Gazzinelli, Giovanni Corrêa-Oliveira, Rodrigo Teixeira-Carvalho, Andréa |
author_sort | Silveira-Lemos, Denise |
collection | PubMed |
description | Acute schistosomiasis is associated with a primary exposure and is more commonly seen in nonimmune individuals traveling through endemic regions. In this study, we have focused on the cytokine profile of T lymphocytes evaluated in circulating leukocytes of acute Schistosomiasis mansoni-infected patients (ACT group) before and after praziquantel treatment (ACT-TR group). Our data demonstrated increased values of total leukocytes, eosinophils, and monocytes in both groups. Interestingly, we have observed that patients treated with praziquantel showed increased values of lymphocytes as compared with noninfected group (NI) or ACT groups. Furthermore, a decrease of neutrophils in ACT-TR was observed when compared to ACT group. Analyses of short-term in vitro whole blood stimulation demonstrated that, regardless of the presence of soluble Schistosoma mansoni eggs antigen (SEA), increased synthesis of IFN-γ and IL-4 by T-cells was observed in the ACT group. Analyses of cytokine profile in CD8 T cells demonstrated higher percentage of IFN-γ and IL-4 cells in both ACT and ACT-TR groups apart from increased percentage of IL-10 cells only in the ACT group. This study is the first one to point out the relevance of CD8 T lymphocytes in the immune response induced during the acute phase of schistosomiasis. |
format | Online Article Text |
id | pubmed-3563181 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-35631812013-02-11 Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy Silveira-Lemos, Denise Fernandes Costa-Silva, Matheus Cardoso de Oliveira Silveira, Amanda Azevedo Batista, Mauricio Alves Oliveira-Fraga, Lúcia Soares Silveira, Alda Maria Barbosa Alvarez, Maria Carolina Martins-Filho, Olindo Assis Gazzinelli, Giovanni Corrêa-Oliveira, Rodrigo Teixeira-Carvalho, Andréa J Parasitol Res Research Article Acute schistosomiasis is associated with a primary exposure and is more commonly seen in nonimmune individuals traveling through endemic regions. In this study, we have focused on the cytokine profile of T lymphocytes evaluated in circulating leukocytes of acute Schistosomiasis mansoni-infected patients (ACT group) before and after praziquantel treatment (ACT-TR group). Our data demonstrated increased values of total leukocytes, eosinophils, and monocytes in both groups. Interestingly, we have observed that patients treated with praziquantel showed increased values of lymphocytes as compared with noninfected group (NI) or ACT groups. Furthermore, a decrease of neutrophils in ACT-TR was observed when compared to ACT group. Analyses of short-term in vitro whole blood stimulation demonstrated that, regardless of the presence of soluble Schistosoma mansoni eggs antigen (SEA), increased synthesis of IFN-γ and IL-4 by T-cells was observed in the ACT group. Analyses of cytokine profile in CD8 T cells demonstrated higher percentage of IFN-γ and IL-4 cells in both ACT and ACT-TR groups apart from increased percentage of IL-10 cells only in the ACT group. This study is the first one to point out the relevance of CD8 T lymphocytes in the immune response induced during the acute phase of schistosomiasis. Hindawi Publishing Corporation 2013 2013-01-20 /pmc/articles/PMC3563181/ /pubmed/23401741 http://dx.doi.org/10.1155/2013/909134 Text en Copyright © 2013 Denise Silveira-Lemos et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Silveira-Lemos, Denise Fernandes Costa-Silva, Matheus Cardoso de Oliveira Silveira, Amanda Azevedo Batista, Mauricio Alves Oliveira-Fraga, Lúcia Soares Silveira, Alda Maria Barbosa Alvarez, Maria Carolina Martins-Filho, Olindo Assis Gazzinelli, Giovanni Corrêa-Oliveira, Rodrigo Teixeira-Carvalho, Andréa Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy |
title | Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy |
title_full | Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy |
title_fullStr | Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy |
title_full_unstemmed | Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy |
title_short | Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy |
title_sort | cytokine pattern of t lymphocytes in acute schistosomiasis mansoni patients following treated praziquantel therapy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563181/ https://www.ncbi.nlm.nih.gov/pubmed/23401741 http://dx.doi.org/10.1155/2013/909134 |
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