Cargando…

MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi

BACKGROUND: MicroRNAs play a vital role in the regulation of gene expression and have been identified in every animal with a sequenced genome examined thus far, except for the placozoan Trichoplax. The genomic repertoires of metazoan microRNAs have become increasingly endorsed as phylogenetic charac...

Descripción completa

Detalles Bibliográficos
Autores principales: Maxwell, Evan K, Ryan, Joseph F, Schnitzler, Christine E, Browne, William E, Baxevanis, Andreas D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563456/
https://www.ncbi.nlm.nih.gov/pubmed/23256903
http://dx.doi.org/10.1186/1471-2164-13-714
_version_ 1782258187712331776
author Maxwell, Evan K
Ryan, Joseph F
Schnitzler, Christine E
Browne, William E
Baxevanis, Andreas D
author_facet Maxwell, Evan K
Ryan, Joseph F
Schnitzler, Christine E
Browne, William E
Baxevanis, Andreas D
author_sort Maxwell, Evan K
collection PubMed
description BACKGROUND: MicroRNAs play a vital role in the regulation of gene expression and have been identified in every animal with a sequenced genome examined thus far, except for the placozoan Trichoplax. The genomic repertoires of metazoan microRNAs have become increasingly endorsed as phylogenetic characters and drivers of biological complexity. RESULTS: In this study, we report the first investigation of microRNAs in a species from the phylum Ctenophora. We use short RNA sequencing and the assembled genome of the lobate ctenophore Mnemiopsis leidyi to show that this species appears to lack any recognizable microRNAs, as well as the nuclear proteins Drosha and Pasha, which are critical to canonical microRNA biogenesis. This finding represents the first reported case of a metazoan lacking a Drosha protein. CONCLUSIONS: Recent phylogenomic analyses suggest that Mnemiopsis may be the earliest branching metazoan lineage. If this is true, then the origins of canonical microRNA biogenesis and microRNA-mediated gene regulation may postdate the last common metazoan ancestor. Alternatively, canonical microRNA functionality may have been lost independently in the lineages leading to both Mnemiopsis and the placozoan Trichoplax, suggesting that microRNA functionality was not critical until much later in metazoan evolution.
format Online
Article
Text
id pubmed-3563456
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-35634562013-02-08 MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi Maxwell, Evan K Ryan, Joseph F Schnitzler, Christine E Browne, William E Baxevanis, Andreas D BMC Genomics Research Article BACKGROUND: MicroRNAs play a vital role in the regulation of gene expression and have been identified in every animal with a sequenced genome examined thus far, except for the placozoan Trichoplax. The genomic repertoires of metazoan microRNAs have become increasingly endorsed as phylogenetic characters and drivers of biological complexity. RESULTS: In this study, we report the first investigation of microRNAs in a species from the phylum Ctenophora. We use short RNA sequencing and the assembled genome of the lobate ctenophore Mnemiopsis leidyi to show that this species appears to lack any recognizable microRNAs, as well as the nuclear proteins Drosha and Pasha, which are critical to canonical microRNA biogenesis. This finding represents the first reported case of a metazoan lacking a Drosha protein. CONCLUSIONS: Recent phylogenomic analyses suggest that Mnemiopsis may be the earliest branching metazoan lineage. If this is true, then the origins of canonical microRNA biogenesis and microRNA-mediated gene regulation may postdate the last common metazoan ancestor. Alternatively, canonical microRNA functionality may have been lost independently in the lineages leading to both Mnemiopsis and the placozoan Trichoplax, suggesting that microRNA functionality was not critical until much later in metazoan evolution. BioMed Central 2012-12-20 /pmc/articles/PMC3563456/ /pubmed/23256903 http://dx.doi.org/10.1186/1471-2164-13-714 Text en Copyright ©2012 Maxwell et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Maxwell, Evan K
Ryan, Joseph F
Schnitzler, Christine E
Browne, William E
Baxevanis, Andreas D
MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi
title MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi
title_full MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi
title_fullStr MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi
title_full_unstemmed MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi
title_short MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi
title_sort micrornas and essential components of the microrna processing machinery are not encoded in the genome of the ctenophore mnemiopsis leidyi
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563456/
https://www.ncbi.nlm.nih.gov/pubmed/23256903
http://dx.doi.org/10.1186/1471-2164-13-714
work_keys_str_mv AT maxwellevank micrornasandessentialcomponentsofthemicrornaprocessingmachineryarenotencodedinthegenomeofthectenophoremnemiopsisleidyi
AT ryanjosephf micrornasandessentialcomponentsofthemicrornaprocessingmachineryarenotencodedinthegenomeofthectenophoremnemiopsisleidyi
AT schnitzlerchristinee micrornasandessentialcomponentsofthemicrornaprocessingmachineryarenotencodedinthegenomeofthectenophoremnemiopsisleidyi
AT brownewilliame micrornasandessentialcomponentsofthemicrornaprocessingmachineryarenotencodedinthegenomeofthectenophoremnemiopsisleidyi
AT baxevanisandreasd micrornasandessentialcomponentsofthemicrornaprocessingmachineryarenotencodedinthegenomeofthectenophoremnemiopsisleidyi