2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts

There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is rob...

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Detalles Bibliográficos
Autores principales: Leguia, Mariana, Brophy, Jennifer AN, Densmore, Douglas, Asante, Angel, Anderson, J Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563576/
https://www.ncbi.nlm.nih.gov/pubmed/23305072
http://dx.doi.org/10.1186/1754-1611-7-2
Descripción
Sumario:There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.

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