2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts

There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is rob...

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Detalles Bibliográficos
Autores principales: Leguia, Mariana, Brophy, Jennifer AN, Densmore, Douglas, Asante, Angel, Anderson, J Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563576/
https://www.ncbi.nlm.nih.gov/pubmed/23305072
http://dx.doi.org/10.1186/1754-1611-7-2
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author Leguia, Mariana
Brophy, Jennifer AN
Densmore, Douglas
Asante, Angel
Anderson, J Christopher
author_facet Leguia, Mariana
Brophy, Jennifer AN
Densmore, Douglas
Asante, Angel
Anderson, J Christopher
author_sort Leguia, Mariana
collection PubMed
description There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity.
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spelling pubmed-35635762013-02-08 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts Leguia, Mariana Brophy, Jennifer AN Densmore, Douglas Asante, Angel Anderson, J Christopher J Biol Eng Methodology There is growing demand for robust DNA assembly strategies to quickly and accurately fabricate genetic circuits for synthetic biology. One application of this technology is reconstitution of multi-gene assemblies. Here, we integrate a new software tool chain with 2ab assembly and show that it is robust enough to generate 528 distinct composite parts with an error-free success rate of 96%. Finally, we discuss our findings in the context of its implications for biosafety and biosecurity. BioMed Central 2013-01-10 /pmc/articles/PMC3563576/ /pubmed/23305072 http://dx.doi.org/10.1186/1754-1611-7-2 Text en Copyright ©2013 Leguia et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Leguia, Mariana
Brophy, Jennifer AN
Densmore, Douglas
Asante, Angel
Anderson, J Christopher
2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts
title 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts
title_full 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts
title_fullStr 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts
title_full_unstemmed 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts
title_short 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts
title_sort 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563576/
https://www.ncbi.nlm.nih.gov/pubmed/23305072
http://dx.doi.org/10.1186/1754-1611-7-2
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