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Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast

Core components of cytokinesis are conserved from yeast to human, but how these components are assembled into a robust machine that drives cytokinesis remains poorly understood. In this paper, we show by fluorescence recovery after photobleaching analysis that Myo1, the sole myosin-II in budding yea...

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Autores principales: Wloka, Carsten, Vallen, Elizabeth A., Thé, Lydia, Fang, Xiaodong, Oh, Younghoon, Bi, Erfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563683/
https://www.ncbi.nlm.nih.gov/pubmed/23358243
http://dx.doi.org/10.1083/jcb.201208030
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author Wloka, Carsten
Vallen, Elizabeth A.
Thé, Lydia
Fang, Xiaodong
Oh, Younghoon
Bi, Erfei
author_facet Wloka, Carsten
Vallen, Elizabeth A.
Thé, Lydia
Fang, Xiaodong
Oh, Younghoon
Bi, Erfei
author_sort Wloka, Carsten
collection PubMed
description Core components of cytokinesis are conserved from yeast to human, but how these components are assembled into a robust machine that drives cytokinesis remains poorly understood. In this paper, we show by fluorescence recovery after photobleaching analysis that Myo1, the sole myosin-II in budding yeast, was mobile at the division site before anaphase and became immobilized shortly before cytokinesis. This immobility was independent of actin filaments or the motor domain of Myo1 but required a small region in the Myo1 tail that is thought to be involved in higher-order assembly. As expected, proteins involved in actin ring assembly (tropomyosin and formin) and membrane trafficking (myosin-V and exocyst) were dynamic during cytokinesis. Strikingly, proteins involved in septum formation (the chitin synthase Chs2) and/or its coordination with the actomyosin ring (essential light chain, IQGAP, F-BAR, etc.) displayed Myo1-dependent immobility during cytokinesis, suggesting that Myo1 plays a scaffolding role in the assembly of a cytokinesis machine.
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spelling pubmed-35636832013-08-04 Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast Wloka, Carsten Vallen, Elizabeth A. Thé, Lydia Fang, Xiaodong Oh, Younghoon Bi, Erfei J Cell Biol Research Articles Core components of cytokinesis are conserved from yeast to human, but how these components are assembled into a robust machine that drives cytokinesis remains poorly understood. In this paper, we show by fluorescence recovery after photobleaching analysis that Myo1, the sole myosin-II in budding yeast, was mobile at the division site before anaphase and became immobilized shortly before cytokinesis. This immobility was independent of actin filaments or the motor domain of Myo1 but required a small region in the Myo1 tail that is thought to be involved in higher-order assembly. As expected, proteins involved in actin ring assembly (tropomyosin and formin) and membrane trafficking (myosin-V and exocyst) were dynamic during cytokinesis. Strikingly, proteins involved in septum formation (the chitin synthase Chs2) and/or its coordination with the actomyosin ring (essential light chain, IQGAP, F-BAR, etc.) displayed Myo1-dependent immobility during cytokinesis, suggesting that Myo1 plays a scaffolding role in the assembly of a cytokinesis machine. The Rockefeller University Press 2013-02-04 /pmc/articles/PMC3563683/ /pubmed/23358243 http://dx.doi.org/10.1083/jcb.201208030 Text en © 2013 Wloka et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Wloka, Carsten
Vallen, Elizabeth A.
Thé, Lydia
Fang, Xiaodong
Oh, Younghoon
Bi, Erfei
Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast
title Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast
title_full Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast
title_fullStr Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast
title_full_unstemmed Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast
title_short Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast
title_sort immobile myosin-ii plays a scaffolding role during cytokinesis in budding yeast
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563683/
https://www.ncbi.nlm.nih.gov/pubmed/23358243
http://dx.doi.org/10.1083/jcb.201208030
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