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Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast
Core components of cytokinesis are conserved from yeast to human, but how these components are assembled into a robust machine that drives cytokinesis remains poorly understood. In this paper, we show by fluorescence recovery after photobleaching analysis that Myo1, the sole myosin-II in budding yea...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563683/ https://www.ncbi.nlm.nih.gov/pubmed/23358243 http://dx.doi.org/10.1083/jcb.201208030 |
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author | Wloka, Carsten Vallen, Elizabeth A. Thé, Lydia Fang, Xiaodong Oh, Younghoon Bi, Erfei |
author_facet | Wloka, Carsten Vallen, Elizabeth A. Thé, Lydia Fang, Xiaodong Oh, Younghoon Bi, Erfei |
author_sort | Wloka, Carsten |
collection | PubMed |
description | Core components of cytokinesis are conserved from yeast to human, but how these components are assembled into a robust machine that drives cytokinesis remains poorly understood. In this paper, we show by fluorescence recovery after photobleaching analysis that Myo1, the sole myosin-II in budding yeast, was mobile at the division site before anaphase and became immobilized shortly before cytokinesis. This immobility was independent of actin filaments or the motor domain of Myo1 but required a small region in the Myo1 tail that is thought to be involved in higher-order assembly. As expected, proteins involved in actin ring assembly (tropomyosin and formin) and membrane trafficking (myosin-V and exocyst) were dynamic during cytokinesis. Strikingly, proteins involved in septum formation (the chitin synthase Chs2) and/or its coordination with the actomyosin ring (essential light chain, IQGAP, F-BAR, etc.) displayed Myo1-dependent immobility during cytokinesis, suggesting that Myo1 plays a scaffolding role in the assembly of a cytokinesis machine. |
format | Online Article Text |
id | pubmed-3563683 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-35636832013-08-04 Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast Wloka, Carsten Vallen, Elizabeth A. Thé, Lydia Fang, Xiaodong Oh, Younghoon Bi, Erfei J Cell Biol Research Articles Core components of cytokinesis are conserved from yeast to human, but how these components are assembled into a robust machine that drives cytokinesis remains poorly understood. In this paper, we show by fluorescence recovery after photobleaching analysis that Myo1, the sole myosin-II in budding yeast, was mobile at the division site before anaphase and became immobilized shortly before cytokinesis. This immobility was independent of actin filaments or the motor domain of Myo1 but required a small region in the Myo1 tail that is thought to be involved in higher-order assembly. As expected, proteins involved in actin ring assembly (tropomyosin and formin) and membrane trafficking (myosin-V and exocyst) were dynamic during cytokinesis. Strikingly, proteins involved in septum formation (the chitin synthase Chs2) and/or its coordination with the actomyosin ring (essential light chain, IQGAP, F-BAR, etc.) displayed Myo1-dependent immobility during cytokinesis, suggesting that Myo1 plays a scaffolding role in the assembly of a cytokinesis machine. The Rockefeller University Press 2013-02-04 /pmc/articles/PMC3563683/ /pubmed/23358243 http://dx.doi.org/10.1083/jcb.201208030 Text en © 2013 Wloka et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Wloka, Carsten Vallen, Elizabeth A. Thé, Lydia Fang, Xiaodong Oh, Younghoon Bi, Erfei Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast |
title | Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast |
title_full | Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast |
title_fullStr | Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast |
title_full_unstemmed | Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast |
title_short | Immobile myosin-II plays a scaffolding role during cytokinesis in budding yeast |
title_sort | immobile myosin-ii plays a scaffolding role during cytokinesis in budding yeast |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3563683/ https://www.ncbi.nlm.nih.gov/pubmed/23358243 http://dx.doi.org/10.1083/jcb.201208030 |
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