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High-throughput sequencing for biology and medicine
Advances in genome sequencing have progressed at a rapid pace, with increased throughput accompanied by plunging costs. But these advances go far beyond faster and cheaper. High-throughput sequencing technologies are now routinely being applied to a wide range of important topics in biology and medi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
European Molecular Biology Organization
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564260/ https://www.ncbi.nlm.nih.gov/pubmed/23340846 http://dx.doi.org/10.1038/msb.2012.61 |
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author | Soon, Wendy Weijia Hariharan, Manoj Snyder, Michael P |
author_facet | Soon, Wendy Weijia Hariharan, Manoj Snyder, Michael P |
author_sort | Soon, Wendy Weijia |
collection | PubMed |
description | Advances in genome sequencing have progressed at a rapid pace, with increased throughput accompanied by plunging costs. But these advances go far beyond faster and cheaper. High-throughput sequencing technologies are now routinely being applied to a wide range of important topics in biology and medicine, often allowing researchers to address important biological questions that were not possible before. In this review, we discuss these innovative new approaches—including ever finer analyses of transcriptome dynamics, genome structure and genomic variation—and provide an overview of the new insights into complex biological systems catalyzed by these technologies. We also assess the impact of genotyping, genome sequencing and personal omics profiling on medical applications, including diagnosis and disease monitoring. Finally, we review recent developments in single-cell sequencing, and conclude with a discussion of possible future advances and obstacles for sequencing in biology and health. |
format | Online Article Text |
id | pubmed-3564260 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | European Molecular Biology Organization |
record_format | MEDLINE/PubMed |
spelling | pubmed-35642602013-02-05 High-throughput sequencing for biology and medicine Soon, Wendy Weijia Hariharan, Manoj Snyder, Michael P Mol Syst Biol Review Article Advances in genome sequencing have progressed at a rapid pace, with increased throughput accompanied by plunging costs. But these advances go far beyond faster and cheaper. High-throughput sequencing technologies are now routinely being applied to a wide range of important topics in biology and medicine, often allowing researchers to address important biological questions that were not possible before. In this review, we discuss these innovative new approaches—including ever finer analyses of transcriptome dynamics, genome structure and genomic variation—and provide an overview of the new insights into complex biological systems catalyzed by these technologies. We also assess the impact of genotyping, genome sequencing and personal omics profiling on medical applications, including diagnosis and disease monitoring. Finally, we review recent developments in single-cell sequencing, and conclude with a discussion of possible future advances and obstacles for sequencing in biology and health. European Molecular Biology Organization 2013-01-22 /pmc/articles/PMC3564260/ /pubmed/23340846 http://dx.doi.org/10.1038/msb.2012.61 Text en Copyright © 2013, EMBO and Macmillan Publishers Limited https://creativecommons.org/licenses/by-nc-sa/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission. |
spellingShingle | Review Article Soon, Wendy Weijia Hariharan, Manoj Snyder, Michael P High-throughput sequencing for biology and medicine |
title | High-throughput sequencing for biology and medicine |
title_full | High-throughput sequencing for biology and medicine |
title_fullStr | High-throughput sequencing for biology and medicine |
title_full_unstemmed | High-throughput sequencing for biology and medicine |
title_short | High-throughput sequencing for biology and medicine |
title_sort | high-throughput sequencing for biology and medicine |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564260/ https://www.ncbi.nlm.nih.gov/pubmed/23340846 http://dx.doi.org/10.1038/msb.2012.61 |
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