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High-throughput sequencing for biology and medicine

Advances in genome sequencing have progressed at a rapid pace, with increased throughput accompanied by plunging costs. But these advances go far beyond faster and cheaper. High-throughput sequencing technologies are now routinely being applied to a wide range of important topics in biology and medi...

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Detalles Bibliográficos
Autores principales: Soon, Wendy Weijia, Hariharan, Manoj, Snyder, Michael P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Molecular Biology Organization 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564260/
https://www.ncbi.nlm.nih.gov/pubmed/23340846
http://dx.doi.org/10.1038/msb.2012.61
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author Soon, Wendy Weijia
Hariharan, Manoj
Snyder, Michael P
author_facet Soon, Wendy Weijia
Hariharan, Manoj
Snyder, Michael P
author_sort Soon, Wendy Weijia
collection PubMed
description Advances in genome sequencing have progressed at a rapid pace, with increased throughput accompanied by plunging costs. But these advances go far beyond faster and cheaper. High-throughput sequencing technologies are now routinely being applied to a wide range of important topics in biology and medicine, often allowing researchers to address important biological questions that were not possible before. In this review, we discuss these innovative new approaches—including ever finer analyses of transcriptome dynamics, genome structure and genomic variation—and provide an overview of the new insights into complex biological systems catalyzed by these technologies. We also assess the impact of genotyping, genome sequencing and personal omics profiling on medical applications, including diagnosis and disease monitoring. Finally, we review recent developments in single-cell sequencing, and conclude with a discussion of possible future advances and obstacles for sequencing in biology and health.
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spelling pubmed-35642602013-02-05 High-throughput sequencing for biology and medicine Soon, Wendy Weijia Hariharan, Manoj Snyder, Michael P Mol Syst Biol Review Article Advances in genome sequencing have progressed at a rapid pace, with increased throughput accompanied by plunging costs. But these advances go far beyond faster and cheaper. High-throughput sequencing technologies are now routinely being applied to a wide range of important topics in biology and medicine, often allowing researchers to address important biological questions that were not possible before. In this review, we discuss these innovative new approaches—including ever finer analyses of transcriptome dynamics, genome structure and genomic variation—and provide an overview of the new insights into complex biological systems catalyzed by these technologies. We also assess the impact of genotyping, genome sequencing and personal omics profiling on medical applications, including diagnosis and disease monitoring. Finally, we review recent developments in single-cell sequencing, and conclude with a discussion of possible future advances and obstacles for sequencing in biology and health. European Molecular Biology Organization 2013-01-22 /pmc/articles/PMC3564260/ /pubmed/23340846 http://dx.doi.org/10.1038/msb.2012.61 Text en Copyright © 2013, EMBO and Macmillan Publishers Limited https://creativecommons.org/licenses/by-nc-sa/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.
spellingShingle Review Article
Soon, Wendy Weijia
Hariharan, Manoj
Snyder, Michael P
High-throughput sequencing for biology and medicine
title High-throughput sequencing for biology and medicine
title_full High-throughput sequencing for biology and medicine
title_fullStr High-throughput sequencing for biology and medicine
title_full_unstemmed High-throughput sequencing for biology and medicine
title_short High-throughput sequencing for biology and medicine
title_sort high-throughput sequencing for biology and medicine
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564260/
https://www.ncbi.nlm.nih.gov/pubmed/23340846
http://dx.doi.org/10.1038/msb.2012.61
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