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Identification of a Novel Jasmonate-Responsive Element in the AtJMT Promoter and Its Binding Protein for AtJMT Repression

Jasmonates (JAs) are important regulators of plant biotic and abiotic stress responses and development. AtJMT in Arabidopsis thaliana and BcNTR1 in Brassica campestris encode jasmonic acid carboxyl methyltransferases, which catalyze methyl jasmonate (MeJA) biosynthesis and are involved in JA signali...

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Autores principales: Seo, Jun Sung, Koo, Yeon Jong, Jung, Choonkyun, Yeu, Song Yion, Song, Jong Tae, Kim, Ju-Kon, Choi, Yeonhee, Lee, Jong Seob, Do Choi, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564755/
https://www.ncbi.nlm.nih.gov/pubmed/23393583
http://dx.doi.org/10.1371/journal.pone.0055482
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author Seo, Jun Sung
Koo, Yeon Jong
Jung, Choonkyun
Yeu, Song Yion
Song, Jong Tae
Kim, Ju-Kon
Choi, Yeonhee
Lee, Jong Seob
Do Choi, Yang
author_facet Seo, Jun Sung
Koo, Yeon Jong
Jung, Choonkyun
Yeu, Song Yion
Song, Jong Tae
Kim, Ju-Kon
Choi, Yeonhee
Lee, Jong Seob
Do Choi, Yang
author_sort Seo, Jun Sung
collection PubMed
description Jasmonates (JAs) are important regulators of plant biotic and abiotic stress responses and development. AtJMT in Arabidopsis thaliana and BcNTR1 in Brassica campestris encode jasmonic acid carboxyl methyltransferases, which catalyze methyl jasmonate (MeJA) biosynthesis and are involved in JA signaling. Their expression is induced by MeJA application. To understand its regulatory mechanism, here we define a novel JA-responsive cis-element (JARE), G(C)TCCTGA, in the AtJMT and BcNTR1 promoters, by promoter deletion analysis and Yeast 1-Hybrid (Y1H) assays; the JARE is distinct from other JA-responsive cis-elements previously reported. We also used Y1H screening to identify a trans-acting factor, AtBBD1, which binds to the JARE and interacts with AtJAZ1 and AtJAZ4. Knockout and overexpression analyses showed that AtBBD1 and its close homologue AtBBD2 are functionally redundant and act as negative regulators of AtJMT expression. However, AtBBD1 positively regulated the JA-responsive expression of JR2. Chromatin immunoprecipitation from knockout and overexpression plants revealed that repression of AtJMT is associated with reduced histone acetylation in the promoter region containing the JARE. These results show that AtBBD1 interacts with JAZ proteins, binds to the JARE and represses AtJMT expression.
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spelling pubmed-35647552013-02-07 Identification of a Novel Jasmonate-Responsive Element in the AtJMT Promoter and Its Binding Protein for AtJMT Repression Seo, Jun Sung Koo, Yeon Jong Jung, Choonkyun Yeu, Song Yion Song, Jong Tae Kim, Ju-Kon Choi, Yeonhee Lee, Jong Seob Do Choi, Yang PLoS One Research Article Jasmonates (JAs) are important regulators of plant biotic and abiotic stress responses and development. AtJMT in Arabidopsis thaliana and BcNTR1 in Brassica campestris encode jasmonic acid carboxyl methyltransferases, which catalyze methyl jasmonate (MeJA) biosynthesis and are involved in JA signaling. Their expression is induced by MeJA application. To understand its regulatory mechanism, here we define a novel JA-responsive cis-element (JARE), G(C)TCCTGA, in the AtJMT and BcNTR1 promoters, by promoter deletion analysis and Yeast 1-Hybrid (Y1H) assays; the JARE is distinct from other JA-responsive cis-elements previously reported. We also used Y1H screening to identify a trans-acting factor, AtBBD1, which binds to the JARE and interacts with AtJAZ1 and AtJAZ4. Knockout and overexpression analyses showed that AtBBD1 and its close homologue AtBBD2 are functionally redundant and act as negative regulators of AtJMT expression. However, AtBBD1 positively regulated the JA-responsive expression of JR2. Chromatin immunoprecipitation from knockout and overexpression plants revealed that repression of AtJMT is associated with reduced histone acetylation in the promoter region containing the JARE. These results show that AtBBD1 interacts with JAZ proteins, binds to the JARE and represses AtJMT expression. Public Library of Science 2013-02-05 /pmc/articles/PMC3564755/ /pubmed/23393583 http://dx.doi.org/10.1371/journal.pone.0055482 Text en © 2013 Seo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Seo, Jun Sung
Koo, Yeon Jong
Jung, Choonkyun
Yeu, Song Yion
Song, Jong Tae
Kim, Ju-Kon
Choi, Yeonhee
Lee, Jong Seob
Do Choi, Yang
Identification of a Novel Jasmonate-Responsive Element in the AtJMT Promoter and Its Binding Protein for AtJMT Repression
title Identification of a Novel Jasmonate-Responsive Element in the AtJMT Promoter and Its Binding Protein for AtJMT Repression
title_full Identification of a Novel Jasmonate-Responsive Element in the AtJMT Promoter and Its Binding Protein for AtJMT Repression
title_fullStr Identification of a Novel Jasmonate-Responsive Element in the AtJMT Promoter and Its Binding Protein for AtJMT Repression
title_full_unstemmed Identification of a Novel Jasmonate-Responsive Element in the AtJMT Promoter and Its Binding Protein for AtJMT Repression
title_short Identification of a Novel Jasmonate-Responsive Element in the AtJMT Promoter and Its Binding Protein for AtJMT Repression
title_sort identification of a novel jasmonate-responsive element in the atjmt promoter and its binding protein for atjmt repression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3564755/
https://www.ncbi.nlm.nih.gov/pubmed/23393583
http://dx.doi.org/10.1371/journal.pone.0055482
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