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Chromosome-Specific DNA Repeats: Rapid Identification in Silico and Validation Using Fluorescence in Situ Hybridization
Chromosome enumeration in interphase and metaphase cells using fluorescence in situ hybridization (FISH) is an established procedure for the rapid and accurate cytogenetic analysis of cell nuclei and polar bodies, the unambiguous gender determination, as well as the definition of tumor-specific sign...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565251/ https://www.ncbi.nlm.nih.gov/pubmed/23344021 http://dx.doi.org/10.3390/ijms14010057 |
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author | Hsu, Joanne H. Zeng, Hui Lemke, Kalistyn H. Polyzos, Aris A. Weier, Jingly F. Wang, Mei Lawin-O’Brien, Anna R. Weier, Heinz-Ulrich G. O’Brien, Benjamin |
author_facet | Hsu, Joanne H. Zeng, Hui Lemke, Kalistyn H. Polyzos, Aris A. Weier, Jingly F. Wang, Mei Lawin-O’Brien, Anna R. Weier, Heinz-Ulrich G. O’Brien, Benjamin |
author_sort | Hsu, Joanne H. |
collection | PubMed |
description | Chromosome enumeration in interphase and metaphase cells using fluorescence in situ hybridization (FISH) is an established procedure for the rapid and accurate cytogenetic analysis of cell nuclei and polar bodies, the unambiguous gender determination, as well as the definition of tumor-specific signatures. Present bottlenecks in the procedure are a limited number of commercial, non-isotopically labeled probes that can be combined in multiplex FISH assays and the relatively high price and effort to develop additional probes. We describe a streamlined approach for rapid probe definition, synthesis and validation, which is based on the analysis of publicly available DNA sequence information, also known as “database mining”. Examples of probe preparation for the human gonosomes and chromosome 16 as a selected autosome outline the probe selection strategy, define a timeline for expedited probe production and compare this novel selection strategy to more conventional probe cloning protocols. |
format | Online Article Text |
id | pubmed-3565251 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-35652512013-03-13 Chromosome-Specific DNA Repeats: Rapid Identification in Silico and Validation Using Fluorescence in Situ Hybridization Hsu, Joanne H. Zeng, Hui Lemke, Kalistyn H. Polyzos, Aris A. Weier, Jingly F. Wang, Mei Lawin-O’Brien, Anna R. Weier, Heinz-Ulrich G. O’Brien, Benjamin Int J Mol Sci Article Chromosome enumeration in interphase and metaphase cells using fluorescence in situ hybridization (FISH) is an established procedure for the rapid and accurate cytogenetic analysis of cell nuclei and polar bodies, the unambiguous gender determination, as well as the definition of tumor-specific signatures. Present bottlenecks in the procedure are a limited number of commercial, non-isotopically labeled probes that can be combined in multiplex FISH assays and the relatively high price and effort to develop additional probes. We describe a streamlined approach for rapid probe definition, synthesis and validation, which is based on the analysis of publicly available DNA sequence information, also known as “database mining”. Examples of probe preparation for the human gonosomes and chromosome 16 as a selected autosome outline the probe selection strategy, define a timeline for expedited probe production and compare this novel selection strategy to more conventional probe cloning protocols. MDPI 2012-12-20 /pmc/articles/PMC3565251/ /pubmed/23344021 http://dx.doi.org/10.3390/ijms14010057 Text en © 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Hsu, Joanne H. Zeng, Hui Lemke, Kalistyn H. Polyzos, Aris A. Weier, Jingly F. Wang, Mei Lawin-O’Brien, Anna R. Weier, Heinz-Ulrich G. O’Brien, Benjamin Chromosome-Specific DNA Repeats: Rapid Identification in Silico and Validation Using Fluorescence in Situ Hybridization |
title | Chromosome-Specific DNA Repeats: Rapid Identification in Silico and Validation Using Fluorescence in Situ Hybridization |
title_full | Chromosome-Specific DNA Repeats: Rapid Identification in Silico and Validation Using Fluorescence in Situ Hybridization |
title_fullStr | Chromosome-Specific DNA Repeats: Rapid Identification in Silico and Validation Using Fluorescence in Situ Hybridization |
title_full_unstemmed | Chromosome-Specific DNA Repeats: Rapid Identification in Silico and Validation Using Fluorescence in Situ Hybridization |
title_short | Chromosome-Specific DNA Repeats: Rapid Identification in Silico and Validation Using Fluorescence in Situ Hybridization |
title_sort | chromosome-specific dna repeats: rapid identification in silico and validation using fluorescence in situ hybridization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565251/ https://www.ncbi.nlm.nih.gov/pubmed/23344021 http://dx.doi.org/10.3390/ijms14010057 |
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