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Expression and Functional Studies on the Noncoding RNA, PRINS

PRINS, a noncoding RNA identified earlier by our research group, contributes to psoriasis susceptibility and cellular stress response. We have now studied the cellular and histological distribution of PRINS by using in situ hybridization and demonstrated variable expressions in different human tissu...

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Autores principales: Szegedi, Krisztina, Göblös, Anikó, Bacsa, Sarolta, Antal, Mária, Németh, István Balázs, Bata-Csörgő, Zsuzsanna, Kemény, Lajos, Dobozy, Attila, Széll, Márta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565259/
https://www.ncbi.nlm.nih.gov/pubmed/23344029
http://dx.doi.org/10.3390/ijms14010205
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author Szegedi, Krisztina
Göblös, Anikó
Bacsa, Sarolta
Antal, Mária
Németh, István Balázs
Bata-Csörgő, Zsuzsanna
Kemény, Lajos
Dobozy, Attila
Széll, Márta
author_facet Szegedi, Krisztina
Göblös, Anikó
Bacsa, Sarolta
Antal, Mária
Németh, István Balázs
Bata-Csörgő, Zsuzsanna
Kemény, Lajos
Dobozy, Attila
Széll, Márta
author_sort Szegedi, Krisztina
collection PubMed
description PRINS, a noncoding RNA identified earlier by our research group, contributes to psoriasis susceptibility and cellular stress response. We have now studied the cellular and histological distribution of PRINS by using in situ hybridization and demonstrated variable expressions in different human tissues and a consistent staining pattern in epidermal keratinocytes and in vitro cultured keratinocytes. To identify the cellular function(s) of PRINS, we searched for a direct interacting partner(s) of this stress-induced molecule. In HaCaT and NHEK cell lysates, the protein proved to be nucleophosmin (NPM) protein as a potential physical interactor with PRINS. Immunohistochemical experiments revealed an elevated expression of NPM in the dividing cells of the basal layers of psoriatic involved skin samples as compared with healthy and psoriatic uninvolved samples. Others have previously shown that NPM is a ubiquitously expressed nucleolar phosphoprotein which shuttles to the nucleoplasm after UV-B irradiation in fibroblasts and cancer cells. We detected a similar translocation of NPM in UV-B-irradiated cultured keratinocytes. The gene-specific silencing of PRINS resulted in the retention of NPM in the nucleolus of UV-B-irradiated keratinocytes; suggesting that PRINS may play a role in the NPM-mediated cellular stress response in the skin.
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spelling pubmed-35652592013-03-13 Expression and Functional Studies on the Noncoding RNA, PRINS Szegedi, Krisztina Göblös, Anikó Bacsa, Sarolta Antal, Mária Németh, István Balázs Bata-Csörgő, Zsuzsanna Kemény, Lajos Dobozy, Attila Széll, Márta Int J Mol Sci Article PRINS, a noncoding RNA identified earlier by our research group, contributes to psoriasis susceptibility and cellular stress response. We have now studied the cellular and histological distribution of PRINS by using in situ hybridization and demonstrated variable expressions in different human tissues and a consistent staining pattern in epidermal keratinocytes and in vitro cultured keratinocytes. To identify the cellular function(s) of PRINS, we searched for a direct interacting partner(s) of this stress-induced molecule. In HaCaT and NHEK cell lysates, the protein proved to be nucleophosmin (NPM) protein as a potential physical interactor with PRINS. Immunohistochemical experiments revealed an elevated expression of NPM in the dividing cells of the basal layers of psoriatic involved skin samples as compared with healthy and psoriatic uninvolved samples. Others have previously shown that NPM is a ubiquitously expressed nucleolar phosphoprotein which shuttles to the nucleoplasm after UV-B irradiation in fibroblasts and cancer cells. We detected a similar translocation of NPM in UV-B-irradiated cultured keratinocytes. The gene-specific silencing of PRINS resulted in the retention of NPM in the nucleolus of UV-B-irradiated keratinocytes; suggesting that PRINS may play a role in the NPM-mediated cellular stress response in the skin. MDPI 2012-12-21 /pmc/articles/PMC3565259/ /pubmed/23344029 http://dx.doi.org/10.3390/ijms14010205 Text en © 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Szegedi, Krisztina
Göblös, Anikó
Bacsa, Sarolta
Antal, Mária
Németh, István Balázs
Bata-Csörgő, Zsuzsanna
Kemény, Lajos
Dobozy, Attila
Széll, Márta
Expression and Functional Studies on the Noncoding RNA, PRINS
title Expression and Functional Studies on the Noncoding RNA, PRINS
title_full Expression and Functional Studies on the Noncoding RNA, PRINS
title_fullStr Expression and Functional Studies on the Noncoding RNA, PRINS
title_full_unstemmed Expression and Functional Studies on the Noncoding RNA, PRINS
title_short Expression and Functional Studies on the Noncoding RNA, PRINS
title_sort expression and functional studies on the noncoding rna, prins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565259/
https://www.ncbi.nlm.nih.gov/pubmed/23344029
http://dx.doi.org/10.3390/ijms14010205
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