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TNF-α Gene Knockout in Triple Negative Breast Cancer Cell Line Induces Apoptosis

Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine involved in the promotion and progression of cancer, including triple negative breast cancer cells. Thus, there is significant interest in understanding the molecular signaling pathways that connect TNF-α with the survival of tumor c...

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Autores principales: Pileczki, Valentina, Braicu, Cornelia, Gherman, Claudia D., Berindan-Neagoe, Ioana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565271/
https://www.ncbi.nlm.nih.gov/pubmed/23263670
http://dx.doi.org/10.3390/ijms14010411
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author Pileczki, Valentina
Braicu, Cornelia
Gherman, Claudia D.
Berindan-Neagoe, Ioana
author_facet Pileczki, Valentina
Braicu, Cornelia
Gherman, Claudia D.
Berindan-Neagoe, Ioana
author_sort Pileczki, Valentina
collection PubMed
description Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine involved in the promotion and progression of cancer, including triple negative breast cancer cells. Thus, there is significant interest in understanding the molecular signaling pathways that connect TNF-α with the survival of tumor cells. In our experiments, we used as an in vitro model for triple negative breast cancer the cell line Hs578T. The purpose of this study is to determine the gene expression profiling of apoptotic signaling networks after blocking TNF-α formation by using specially designed siRNA molecules to target TNF-α messenger RNA. Knockdown of TNF-α gene was associated with cell proliferation inhibition and apoptosis, as observed by monitoring the cell index using the xCELLigence RTCA System and flow cytometry. PCR array technology was used to examine the transcript levels of 84 genes involved in apoptosis. 15 genes were found to be relevant after comparing the treated group with the untreated one of which 3 were down-regulated and 12 up-regulated. The down-regulated genes are all involved in cell survival, whereas the up-regulated ones are involved in and interact with pro-apoptotic pathways. The results described here indicate that the direct target of TNF-α in the Hs578T breast cancer cell line increases the level of certain pro-apoptotic factors that modulate different cellular networks that direct the cells towards death.
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spelling pubmed-35652712013-03-13 TNF-α Gene Knockout in Triple Negative Breast Cancer Cell Line Induces Apoptosis Pileczki, Valentina Braicu, Cornelia Gherman, Claudia D. Berindan-Neagoe, Ioana Int J Mol Sci Article Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine involved in the promotion and progression of cancer, including triple negative breast cancer cells. Thus, there is significant interest in understanding the molecular signaling pathways that connect TNF-α with the survival of tumor cells. In our experiments, we used as an in vitro model for triple negative breast cancer the cell line Hs578T. The purpose of this study is to determine the gene expression profiling of apoptotic signaling networks after blocking TNF-α formation by using specially designed siRNA molecules to target TNF-α messenger RNA. Knockdown of TNF-α gene was associated with cell proliferation inhibition and apoptosis, as observed by monitoring the cell index using the xCELLigence RTCA System and flow cytometry. PCR array technology was used to examine the transcript levels of 84 genes involved in apoptosis. 15 genes were found to be relevant after comparing the treated group with the untreated one of which 3 were down-regulated and 12 up-regulated. The down-regulated genes are all involved in cell survival, whereas the up-regulated ones are involved in and interact with pro-apoptotic pathways. The results described here indicate that the direct target of TNF-α in the Hs578T breast cancer cell line increases the level of certain pro-apoptotic factors that modulate different cellular networks that direct the cells towards death. MDPI 2012-12-24 /pmc/articles/PMC3565271/ /pubmed/23263670 http://dx.doi.org/10.3390/ijms14010411 Text en © 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Pileczki, Valentina
Braicu, Cornelia
Gherman, Claudia D.
Berindan-Neagoe, Ioana
TNF-α Gene Knockout in Triple Negative Breast Cancer Cell Line Induces Apoptosis
title TNF-α Gene Knockout in Triple Negative Breast Cancer Cell Line Induces Apoptosis
title_full TNF-α Gene Knockout in Triple Negative Breast Cancer Cell Line Induces Apoptosis
title_fullStr TNF-α Gene Knockout in Triple Negative Breast Cancer Cell Line Induces Apoptosis
title_full_unstemmed TNF-α Gene Knockout in Triple Negative Breast Cancer Cell Line Induces Apoptosis
title_short TNF-α Gene Knockout in Triple Negative Breast Cancer Cell Line Induces Apoptosis
title_sort tnf-α gene knockout in triple negative breast cancer cell line induces apoptosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565271/
https://www.ncbi.nlm.nih.gov/pubmed/23263670
http://dx.doi.org/10.3390/ijms14010411
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