Cargando…

Identification of Recurrence Related microRNAs in Hepatocellular Carcinoma after Surgical Resection

Hepatocellular carcinoma (HCC) is one of the most aggressive human cancers with a high frequency of post-surgical recurrence. It is very critical to diagnose HCC recurrence at an early stage for a better therapeutic treatment. In this study, we examined the microRNA (miRNA) expression profiling in t...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Zhen, Miao, Ruoyu, Li, Guangbing, Wu, Yan, Robson, Simon C., Yang, Xiaobo, Zhao, Yi, Zhao, Haitao, Zhong, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565310/
https://www.ncbi.nlm.nih.gov/pubmed/23299437
http://dx.doi.org/10.3390/ijms14011105
_version_ 1782258438502350848
author Yang, Zhen
Miao, Ruoyu
Li, Guangbing
Wu, Yan
Robson, Simon C.
Yang, Xiaobo
Zhao, Yi
Zhao, Haitao
Zhong, Yang
author_facet Yang, Zhen
Miao, Ruoyu
Li, Guangbing
Wu, Yan
Robson, Simon C.
Yang, Xiaobo
Zhao, Yi
Zhao, Haitao
Zhong, Yang
author_sort Yang, Zhen
collection PubMed
description Hepatocellular carcinoma (HCC) is one of the most aggressive human cancers with a high frequency of post-surgical recurrence. It is very critical to diagnose HCC recurrence at an early stage for a better therapeutic treatment. In this study, we examined the microRNA (miRNA) expression profiling in tumor tissues obtained from early and late recurrent HCC patients post-resection, using a microarray assay. A total of 32 miRNAs were identified to be differentially expressed during the progression of recurrence. Among these, 16 miRNAs were upregulated and 16 were downregulated. In addition, this miRNA expression signature was further validated by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Moreover, functional annotation of predicted target genes of these recurrent HCC-related miRNAs indicates that multiple biological pathways (i.e., focal adhesion pathway, cancer-related pathways and mitogen-activated protein kinase (MAPK) signaling) that are all critical for cancer development and progression, may participate in the recurrence of HCC. Our data suggest potential molecular mechanisms underpinning miRNA-controlled HCC recurrence, and support the notion that miRNA expression signature and miRNA-based therapy can be useful tools for a better diagnosis and treatment stratification of this disease.
format Online
Article
Text
id pubmed-3565310
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-35653102013-03-13 Identification of Recurrence Related microRNAs in Hepatocellular Carcinoma after Surgical Resection Yang, Zhen Miao, Ruoyu Li, Guangbing Wu, Yan Robson, Simon C. Yang, Xiaobo Zhao, Yi Zhao, Haitao Zhong, Yang Int J Mol Sci Article Hepatocellular carcinoma (HCC) is one of the most aggressive human cancers with a high frequency of post-surgical recurrence. It is very critical to diagnose HCC recurrence at an early stage for a better therapeutic treatment. In this study, we examined the microRNA (miRNA) expression profiling in tumor tissues obtained from early and late recurrent HCC patients post-resection, using a microarray assay. A total of 32 miRNAs were identified to be differentially expressed during the progression of recurrence. Among these, 16 miRNAs were upregulated and 16 were downregulated. In addition, this miRNA expression signature was further validated by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Moreover, functional annotation of predicted target genes of these recurrent HCC-related miRNAs indicates that multiple biological pathways (i.e., focal adhesion pathway, cancer-related pathways and mitogen-activated protein kinase (MAPK) signaling) that are all critical for cancer development and progression, may participate in the recurrence of HCC. Our data suggest potential molecular mechanisms underpinning miRNA-controlled HCC recurrence, and support the notion that miRNA expression signature and miRNA-based therapy can be useful tools for a better diagnosis and treatment stratification of this disease. MDPI 2013-01-08 /pmc/articles/PMC3565310/ /pubmed/23299437 http://dx.doi.org/10.3390/ijms14011105 Text en © 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Yang, Zhen
Miao, Ruoyu
Li, Guangbing
Wu, Yan
Robson, Simon C.
Yang, Xiaobo
Zhao, Yi
Zhao, Haitao
Zhong, Yang
Identification of Recurrence Related microRNAs in Hepatocellular Carcinoma after Surgical Resection
title Identification of Recurrence Related microRNAs in Hepatocellular Carcinoma after Surgical Resection
title_full Identification of Recurrence Related microRNAs in Hepatocellular Carcinoma after Surgical Resection
title_fullStr Identification of Recurrence Related microRNAs in Hepatocellular Carcinoma after Surgical Resection
title_full_unstemmed Identification of Recurrence Related microRNAs in Hepatocellular Carcinoma after Surgical Resection
title_short Identification of Recurrence Related microRNAs in Hepatocellular Carcinoma after Surgical Resection
title_sort identification of recurrence related micrornas in hepatocellular carcinoma after surgical resection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565310/
https://www.ncbi.nlm.nih.gov/pubmed/23299437
http://dx.doi.org/10.3390/ijms14011105
work_keys_str_mv AT yangzhen identificationofrecurrencerelatedmicrornasinhepatocellularcarcinomaaftersurgicalresection
AT miaoruoyu identificationofrecurrencerelatedmicrornasinhepatocellularcarcinomaaftersurgicalresection
AT liguangbing identificationofrecurrencerelatedmicrornasinhepatocellularcarcinomaaftersurgicalresection
AT wuyan identificationofrecurrencerelatedmicrornasinhepatocellularcarcinomaaftersurgicalresection
AT robsonsimonc identificationofrecurrencerelatedmicrornasinhepatocellularcarcinomaaftersurgicalresection
AT yangxiaobo identificationofrecurrencerelatedmicrornasinhepatocellularcarcinomaaftersurgicalresection
AT zhaoyi identificationofrecurrencerelatedmicrornasinhepatocellularcarcinomaaftersurgicalresection
AT zhaohaitao identificationofrecurrencerelatedmicrornasinhepatocellularcarcinomaaftersurgicalresection
AT zhongyang identificationofrecurrencerelatedmicrornasinhepatocellularcarcinomaaftersurgicalresection