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Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents

The development of melanogenic inhibitors is important for the prevention of hyperpigmentation, and, recently, consideration has been given to natural materials or traditionally used ingredients such as Chinese medicine. The aim of this study is the evaluation of a new anti-melanogenic candidate, ka...

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Autores principales: Goh, Myeong-Jin, Lee, Hae-Kwang, Cheng, Liang, Kong, De-Yun, Yeon, Jae-Ho, He, Quan-Quan, Cho, Jun-Cheol, Na, Yong Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565339/
https://www.ncbi.nlm.nih.gov/pubmed/23322017
http://dx.doi.org/10.3390/ijms14011655
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author Goh, Myeong-Jin
Lee, Hae-Kwang
Cheng, Liang
Kong, De-Yun
Yeon, Jae-Ho
He, Quan-Quan
Cho, Jun-Cheol
Na, Yong Joo
author_facet Goh, Myeong-Jin
Lee, Hae-Kwang
Cheng, Liang
Kong, De-Yun
Yeon, Jae-Ho
He, Quan-Quan
Cho, Jun-Cheol
Na, Yong Joo
author_sort Goh, Myeong-Jin
collection PubMed
description The development of melanogenic inhibitors is important for the prevention of hyperpigmentation, and, recently, consideration has been given to natural materials or traditionally used ingredients such as Chinese medicine. The aim of this study is the evaluation of a new anti-melanogenic candidate, kadsuralignan F, from the natural plant Kadsura coccinea, as well as the determination of mechanisms of melanogenesis inhibition at a molecular level. Kadsuralignan F significantly reduced melanin synthesis in a dose-dependent manner in a murine melanocyte cell line and human skin equivalents. There was no direct inhibition on mushroom tyrosinase or cell-extract tyrosinase activity, and mRNA expression of tyrosinase and other melanogenic genes such as tyrosinase-related protein-1 (trp-1) or trp-2 were not affected by kadsuralignan F. Interestingly, the protein level of tyrosinase was dramatically downregulated with kadsuralignan F treatment. We found that a decrease of tyrosinase protein by kadsuralignan F was fully recovered by MG132, a proteasome inhibitor, but not by chloroquine, a lysosome inhibitor. In this study, we found that kadsuralignan F, a lignan from an extract of Kadsura coccinea, has an inhibitory activity on melanin synthesis through tyrosinase degradation. These findings suggest that kadsuralignan F can be used as an active ingredient for hyperpigmentation treatment.
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spelling pubmed-35653392013-03-13 Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents Goh, Myeong-Jin Lee, Hae-Kwang Cheng, Liang Kong, De-Yun Yeon, Jae-Ho He, Quan-Quan Cho, Jun-Cheol Na, Yong Joo Int J Mol Sci Article The development of melanogenic inhibitors is important for the prevention of hyperpigmentation, and, recently, consideration has been given to natural materials or traditionally used ingredients such as Chinese medicine. The aim of this study is the evaluation of a new anti-melanogenic candidate, kadsuralignan F, from the natural plant Kadsura coccinea, as well as the determination of mechanisms of melanogenesis inhibition at a molecular level. Kadsuralignan F significantly reduced melanin synthesis in a dose-dependent manner in a murine melanocyte cell line and human skin equivalents. There was no direct inhibition on mushroom tyrosinase or cell-extract tyrosinase activity, and mRNA expression of tyrosinase and other melanogenic genes such as tyrosinase-related protein-1 (trp-1) or trp-2 were not affected by kadsuralignan F. Interestingly, the protein level of tyrosinase was dramatically downregulated with kadsuralignan F treatment. We found that a decrease of tyrosinase protein by kadsuralignan F was fully recovered by MG132, a proteasome inhibitor, but not by chloroquine, a lysosome inhibitor. In this study, we found that kadsuralignan F, a lignan from an extract of Kadsura coccinea, has an inhibitory activity on melanin synthesis through tyrosinase degradation. These findings suggest that kadsuralignan F can be used as an active ingredient for hyperpigmentation treatment. MDPI 2013-01-15 /pmc/articles/PMC3565339/ /pubmed/23322017 http://dx.doi.org/10.3390/ijms14011655 Text en © 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Goh, Myeong-Jin
Lee, Hae-Kwang
Cheng, Liang
Kong, De-Yun
Yeon, Jae-Ho
He, Quan-Quan
Cho, Jun-Cheol
Na, Yong Joo
Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents
title Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents
title_full Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents
title_fullStr Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents
title_full_unstemmed Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents
title_short Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents
title_sort depigmentation effect of kadsuralignan f on melan-a murine melanocytes and human skin equivalents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565339/
https://www.ncbi.nlm.nih.gov/pubmed/23322017
http://dx.doi.org/10.3390/ijms14011655
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