Cargando…
Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents
The development of melanogenic inhibitors is important for the prevention of hyperpigmentation, and, recently, consideration has been given to natural materials or traditionally used ingredients such as Chinese medicine. The aim of this study is the evaluation of a new anti-melanogenic candidate, ka...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565339/ https://www.ncbi.nlm.nih.gov/pubmed/23322017 http://dx.doi.org/10.3390/ijms14011655 |
_version_ | 1782258445280346112 |
---|---|
author | Goh, Myeong-Jin Lee, Hae-Kwang Cheng, Liang Kong, De-Yun Yeon, Jae-Ho He, Quan-Quan Cho, Jun-Cheol Na, Yong Joo |
author_facet | Goh, Myeong-Jin Lee, Hae-Kwang Cheng, Liang Kong, De-Yun Yeon, Jae-Ho He, Quan-Quan Cho, Jun-Cheol Na, Yong Joo |
author_sort | Goh, Myeong-Jin |
collection | PubMed |
description | The development of melanogenic inhibitors is important for the prevention of hyperpigmentation, and, recently, consideration has been given to natural materials or traditionally used ingredients such as Chinese medicine. The aim of this study is the evaluation of a new anti-melanogenic candidate, kadsuralignan F, from the natural plant Kadsura coccinea, as well as the determination of mechanisms of melanogenesis inhibition at a molecular level. Kadsuralignan F significantly reduced melanin synthesis in a dose-dependent manner in a murine melanocyte cell line and human skin equivalents. There was no direct inhibition on mushroom tyrosinase or cell-extract tyrosinase activity, and mRNA expression of tyrosinase and other melanogenic genes such as tyrosinase-related protein-1 (trp-1) or trp-2 were not affected by kadsuralignan F. Interestingly, the protein level of tyrosinase was dramatically downregulated with kadsuralignan F treatment. We found that a decrease of tyrosinase protein by kadsuralignan F was fully recovered by MG132, a proteasome inhibitor, but not by chloroquine, a lysosome inhibitor. In this study, we found that kadsuralignan F, a lignan from an extract of Kadsura coccinea, has an inhibitory activity on melanin synthesis through tyrosinase degradation. These findings suggest that kadsuralignan F can be used as an active ingredient for hyperpigmentation treatment. |
format | Online Article Text |
id | pubmed-3565339 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-35653392013-03-13 Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents Goh, Myeong-Jin Lee, Hae-Kwang Cheng, Liang Kong, De-Yun Yeon, Jae-Ho He, Quan-Quan Cho, Jun-Cheol Na, Yong Joo Int J Mol Sci Article The development of melanogenic inhibitors is important for the prevention of hyperpigmentation, and, recently, consideration has been given to natural materials or traditionally used ingredients such as Chinese medicine. The aim of this study is the evaluation of a new anti-melanogenic candidate, kadsuralignan F, from the natural plant Kadsura coccinea, as well as the determination of mechanisms of melanogenesis inhibition at a molecular level. Kadsuralignan F significantly reduced melanin synthesis in a dose-dependent manner in a murine melanocyte cell line and human skin equivalents. There was no direct inhibition on mushroom tyrosinase or cell-extract tyrosinase activity, and mRNA expression of tyrosinase and other melanogenic genes such as tyrosinase-related protein-1 (trp-1) or trp-2 were not affected by kadsuralignan F. Interestingly, the protein level of tyrosinase was dramatically downregulated with kadsuralignan F treatment. We found that a decrease of tyrosinase protein by kadsuralignan F was fully recovered by MG132, a proteasome inhibitor, but not by chloroquine, a lysosome inhibitor. In this study, we found that kadsuralignan F, a lignan from an extract of Kadsura coccinea, has an inhibitory activity on melanin synthesis through tyrosinase degradation. These findings suggest that kadsuralignan F can be used as an active ingredient for hyperpigmentation treatment. MDPI 2013-01-15 /pmc/articles/PMC3565339/ /pubmed/23322017 http://dx.doi.org/10.3390/ijms14011655 Text en © 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Goh, Myeong-Jin Lee, Hae-Kwang Cheng, Liang Kong, De-Yun Yeon, Jae-Ho He, Quan-Quan Cho, Jun-Cheol Na, Yong Joo Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents |
title | Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents |
title_full | Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents |
title_fullStr | Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents |
title_full_unstemmed | Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents |
title_short | Depigmentation Effect of Kadsuralignan F on Melan-A Murine Melanocytes and Human Skin Equivalents |
title_sort | depigmentation effect of kadsuralignan f on melan-a murine melanocytes and human skin equivalents |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565339/ https://www.ncbi.nlm.nih.gov/pubmed/23322017 http://dx.doi.org/10.3390/ijms14011655 |
work_keys_str_mv | AT gohmyeongjin depigmentationeffectofkadsuralignanfonmelanamurinemelanocytesandhumanskinequivalents AT leehaekwang depigmentationeffectofkadsuralignanfonmelanamurinemelanocytesandhumanskinequivalents AT chengliang depigmentationeffectofkadsuralignanfonmelanamurinemelanocytesandhumanskinequivalents AT kongdeyun depigmentationeffectofkadsuralignanfonmelanamurinemelanocytesandhumanskinequivalents AT yeonjaeho depigmentationeffectofkadsuralignanfonmelanamurinemelanocytesandhumanskinequivalents AT hequanquan depigmentationeffectofkadsuralignanfonmelanamurinemelanocytesandhumanskinequivalents AT chojuncheol depigmentationeffectofkadsuralignanfonmelanamurinemelanocytesandhumanskinequivalents AT nayongjoo depigmentationeffectofkadsuralignanfonmelanamurinemelanocytesandhumanskinequivalents |