Gamma-Aminobutyric Acid Production Using Immobilized Glutamate Decarboxylase Followed by Downstream Processing with Cation Exchange Chromatography

We have developed a gamma-aminobutyric acid (GABA) production technique using his-tag mediated immobilization of Escherichia coli-derived glutamate decarboxylase (GAD), an enzyme that catalyzes the conversion of glutamate to GABA. The GAD was obtained at 1.43 g/L from GAD-overexpressed E. coli ferme...

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Detalles Bibliográficos
Autores principales: Lee, Seungwoon, Ahn, Jungoh, Kim, Yeon-Gu, Jung, Joon-Ki, Lee, Hongweon, Lee, Eun Gyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565344/
https://www.ncbi.nlm.nih.gov/pubmed/23322022
http://dx.doi.org/10.3390/ijms14011728

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565344/
https://www.ncbi.nlm.nih.gov/pubmed/23322022
http://dx.doi.org/10.3390/ijms14011728

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