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Classical swine fever virus failed to activate nuclear factor-kappa b signaling pathway both in vitro and in vivo

BACKGROUND: Classical swine fever virus (CSFV) is the cause of CSF which is a severe disease of pigs, leading to heavy economic losses in many regions of the world. Nuclear factor-kappa B (NF-κB) is a critical regulator of innate and adaptive immunity, and commonly activated upon viral infection. In...

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Autores principales: Chen, Li-Jun, Dong, Xiao-Ying, Zhao, Ming-Qiu, Shen, Hai-Yan, Wang, Jia-Ying, Pei, Jing-Jing, Liu, Wen-Jun, Luo, Yong-Wen, Ju, Chun-Mei, Chen, Jin-Ding
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565942/
https://www.ncbi.nlm.nih.gov/pubmed/23186553
http://dx.doi.org/10.1186/1743-422X-9-293
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author Chen, Li-Jun
Dong, Xiao-Ying
Zhao, Ming-Qiu
Shen, Hai-Yan
Wang, Jia-Ying
Pei, Jing-Jing
Liu, Wen-Jun
Luo, Yong-Wen
Ju, Chun-Mei
Chen, Jin-Ding
author_facet Chen, Li-Jun
Dong, Xiao-Ying
Zhao, Ming-Qiu
Shen, Hai-Yan
Wang, Jia-Ying
Pei, Jing-Jing
Liu, Wen-Jun
Luo, Yong-Wen
Ju, Chun-Mei
Chen, Jin-Ding
author_sort Chen, Li-Jun
collection PubMed
description BACKGROUND: Classical swine fever virus (CSFV) is the cause of CSF which is a severe disease of pigs, leading to heavy economic losses in many regions of the world. Nuclear factor-kappa B (NF-κB) is a critical regulator of innate and adaptive immunity, and commonly activated upon viral infection. In our previous study, we found that CSFV could suppress the maturation and modulate the functions of monocyte-derived dendritic cells (Mo-DCs) without activating NF-κB pathway. To further prove the effects of CSFV on the NF-κB signaling pathway, we investigated the activity of NF-κB after CSFV infection in vivo and in vitro. METHODS: Attenuated Thiverval strain and virulent wild-type GXW-07 strain were used as challenge viruses in this study. Porcine kidney 15 (PK-15) cells were cultured in vitro and peripheral blood mononuclear cells (PBMCs) were isolated from the blood of CSFV-infected pigs. DNA binding of NF-κB was measured by electrophoretic mobility shift assays (EMSA), NF-κB p65 translocation was detected using immunofluorescent staining, and p65/RelA and IκBα expression was measured by Western Blotting. RESULTS: Infection of cells with CSFV in vitro and in vivo showed that compared with tumor necrosis factor alpha (TNF-α) stimulated cells, there was no distinct DNA binding band of NF-κB, and no significant translocation of p65/RelA from the cytoplasm to the nucleus was observed, which might have been due to the apparent lack of IkBa degradation. CONCLUSIONS: CSFV infection had no effect on the NF-κB signaling pathway, indicating that CSFV could evade host activation of NF-κB during infection.
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spelling pubmed-35659422013-02-11 Classical swine fever virus failed to activate nuclear factor-kappa b signaling pathway both in vitro and in vivo Chen, Li-Jun Dong, Xiao-Ying Zhao, Ming-Qiu Shen, Hai-Yan Wang, Jia-Ying Pei, Jing-Jing Liu, Wen-Jun Luo, Yong-Wen Ju, Chun-Mei Chen, Jin-Ding Virol J Research BACKGROUND: Classical swine fever virus (CSFV) is the cause of CSF which is a severe disease of pigs, leading to heavy economic losses in many regions of the world. Nuclear factor-kappa B (NF-κB) is a critical regulator of innate and adaptive immunity, and commonly activated upon viral infection. In our previous study, we found that CSFV could suppress the maturation and modulate the functions of monocyte-derived dendritic cells (Mo-DCs) without activating NF-κB pathway. To further prove the effects of CSFV on the NF-κB signaling pathway, we investigated the activity of NF-κB after CSFV infection in vivo and in vitro. METHODS: Attenuated Thiverval strain and virulent wild-type GXW-07 strain were used as challenge viruses in this study. Porcine kidney 15 (PK-15) cells were cultured in vitro and peripheral blood mononuclear cells (PBMCs) were isolated from the blood of CSFV-infected pigs. DNA binding of NF-κB was measured by electrophoretic mobility shift assays (EMSA), NF-κB p65 translocation was detected using immunofluorescent staining, and p65/RelA and IκBα expression was measured by Western Blotting. RESULTS: Infection of cells with CSFV in vitro and in vivo showed that compared with tumor necrosis factor alpha (TNF-α) stimulated cells, there was no distinct DNA binding band of NF-κB, and no significant translocation of p65/RelA from the cytoplasm to the nucleus was observed, which might have been due to the apparent lack of IkBa degradation. CONCLUSIONS: CSFV infection had no effect on the NF-κB signaling pathway, indicating that CSFV could evade host activation of NF-κB during infection. BioMed Central 2012-11-27 /pmc/articles/PMC3565942/ /pubmed/23186553 http://dx.doi.org/10.1186/1743-422X-9-293 Text en Copyright ©2012 Chen et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Chen, Li-Jun
Dong, Xiao-Ying
Zhao, Ming-Qiu
Shen, Hai-Yan
Wang, Jia-Ying
Pei, Jing-Jing
Liu, Wen-Jun
Luo, Yong-Wen
Ju, Chun-Mei
Chen, Jin-Ding
Classical swine fever virus failed to activate nuclear factor-kappa b signaling pathway both in vitro and in vivo
title Classical swine fever virus failed to activate nuclear factor-kappa b signaling pathway both in vitro and in vivo
title_full Classical swine fever virus failed to activate nuclear factor-kappa b signaling pathway both in vitro and in vivo
title_fullStr Classical swine fever virus failed to activate nuclear factor-kappa b signaling pathway both in vitro and in vivo
title_full_unstemmed Classical swine fever virus failed to activate nuclear factor-kappa b signaling pathway both in vitro and in vivo
title_short Classical swine fever virus failed to activate nuclear factor-kappa b signaling pathway both in vitro and in vivo
title_sort classical swine fever virus failed to activate nuclear factor-kappa b signaling pathway both in vitro and in vivo
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565942/
https://www.ncbi.nlm.nih.gov/pubmed/23186553
http://dx.doi.org/10.1186/1743-422X-9-293
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