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The impact of reference gene selection in quantification of gene expression levels in guinea pig cervical tissues and cells

BACKGROUND: Accurate measurements of mRNA expression levels in tissues or cells are crucially dependent on the use of relevant reference genes for normalization of data. In this study we used quantitative real-time PCR and two Excel-based applets (geNorm and BestKeeper) to determine the best referen...

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Detalles Bibliográficos
Autores principales: Lindqvist, Annika, Manders, Dustin, Word, R Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565975/
https://www.ncbi.nlm.nih.gov/pubmed/23363446
http://dx.doi.org/10.1186/1756-0500-6-34