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The Channel-Activating Protease CAP1/Prss8 Is Required for Placental Labyrinth Maturation
The serine protease CAP1/Prss8 is crucial for skin barrier function, lung alveolar fluid clearance and has been unveiled as diagnostic marker for specific cancer types. Here, we show that a constitutive knockout of CAP1/Prss8 leads to embryonic lethality. These embryos presented no specific defects,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565977/ https://www.ncbi.nlm.nih.gov/pubmed/23405214 http://dx.doi.org/10.1371/journal.pone.0055796 |
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author | Hummler, Edith Dousse, Aline Rieder, Audrey Stehle, Jean-Christophe Rubera, Isabelle Osterheld, Maria-Chiara Beermann, Friedrich Frateschi, Simona Charles, Roch-Philippe |
author_facet | Hummler, Edith Dousse, Aline Rieder, Audrey Stehle, Jean-Christophe Rubera, Isabelle Osterheld, Maria-Chiara Beermann, Friedrich Frateschi, Simona Charles, Roch-Philippe |
author_sort | Hummler, Edith |
collection | PubMed |
description | The serine protease CAP1/Prss8 is crucial for skin barrier function, lung alveolar fluid clearance and has been unveiled as diagnostic marker for specific cancer types. Here, we show that a constitutive knockout of CAP1/Prss8 leads to embryonic lethality. These embryos presented no specific defects, but it is during this period, and in particular at E13.5, that wildtype placentas show an increased expression of CAP1/Prss8, thus suggesting a placental defect in the knockout situation. The placentas of knockout embryos exhibited significantly reduced vascular development and incomplete cellular maturation. In contrary, epiblast-specific deletion of CAP1/Prss8 allowed development until birth. These CAP1/Prss8-deficient newborns presented abnormal epidermis, and died soon after birth due to impaired skin function. We thus conclude that a late placental insufficiency might be the primary cause of embryonic lethality in CAP1/Prss8 knockouts. This study highlights a novel and crucial role for CAP1/Prss8 in placental development and function. |
format | Online Article Text |
id | pubmed-3565977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35659772013-02-12 The Channel-Activating Protease CAP1/Prss8 Is Required for Placental Labyrinth Maturation Hummler, Edith Dousse, Aline Rieder, Audrey Stehle, Jean-Christophe Rubera, Isabelle Osterheld, Maria-Chiara Beermann, Friedrich Frateschi, Simona Charles, Roch-Philippe PLoS One Research Article The serine protease CAP1/Prss8 is crucial for skin barrier function, lung alveolar fluid clearance and has been unveiled as diagnostic marker for specific cancer types. Here, we show that a constitutive knockout of CAP1/Prss8 leads to embryonic lethality. These embryos presented no specific defects, but it is during this period, and in particular at E13.5, that wildtype placentas show an increased expression of CAP1/Prss8, thus suggesting a placental defect in the knockout situation. The placentas of knockout embryos exhibited significantly reduced vascular development and incomplete cellular maturation. In contrary, epiblast-specific deletion of CAP1/Prss8 allowed development until birth. These CAP1/Prss8-deficient newborns presented abnormal epidermis, and died soon after birth due to impaired skin function. We thus conclude that a late placental insufficiency might be the primary cause of embryonic lethality in CAP1/Prss8 knockouts. This study highlights a novel and crucial role for CAP1/Prss8 in placental development and function. Public Library of Science 2013-02-06 /pmc/articles/PMC3565977/ /pubmed/23405214 http://dx.doi.org/10.1371/journal.pone.0055796 Text en © 2013 Hummler et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hummler, Edith Dousse, Aline Rieder, Audrey Stehle, Jean-Christophe Rubera, Isabelle Osterheld, Maria-Chiara Beermann, Friedrich Frateschi, Simona Charles, Roch-Philippe The Channel-Activating Protease CAP1/Prss8 Is Required for Placental Labyrinth Maturation |
title | The Channel-Activating Protease CAP1/Prss8 Is Required for Placental Labyrinth Maturation |
title_full | The Channel-Activating Protease CAP1/Prss8 Is Required for Placental Labyrinth Maturation |
title_fullStr | The Channel-Activating Protease CAP1/Prss8 Is Required for Placental Labyrinth Maturation |
title_full_unstemmed | The Channel-Activating Protease CAP1/Prss8 Is Required for Placental Labyrinth Maturation |
title_short | The Channel-Activating Protease CAP1/Prss8 Is Required for Placental Labyrinth Maturation |
title_sort | channel-activating protease cap1/prss8 is required for placental labyrinth maturation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565977/ https://www.ncbi.nlm.nih.gov/pubmed/23405214 http://dx.doi.org/10.1371/journal.pone.0055796 |
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