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Expanding the Repertoire of Gene Tools for Precise Manipulation of the Clostridium difficile Genome: Allelic Exchange Using pyrE Alleles

Sophisticated genetic tools to modify essential biological processes at the molecular level are pivotal in elucidating the molecular pathogenesis of Clostridium difficile, a major cause of healthcare associated disease. Here we have developed an efficient procedure for making precise alterations to...

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Detalles Bibliográficos
Autores principales:	Ng, Yen Kuan, Ehsaan, Muhammad, Philip, Sheryl, Collery, Mark M., Janoir, Clare, Collignon, Anne, Cartman, Stephen T., Minton, Nigel P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566075/ https://www.ncbi.nlm.nih.gov/pubmed/23405251 http://dx.doi.org/10.1371/journal.pone.0056051

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