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Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells
The phorbol ester Prostratin may either stimulate or inhibit human immunodeficiency virus-1 (HIV-1) replication. Here we report that Prostratin also exhibits a similar dual action upon feline immunodeficiency virus (FIV) replication in an IL-2-dependent feline CD4(+) T-cell line (MYA-1). While withd...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566544/ https://www.ncbi.nlm.nih.gov/pubmed/23201205 http://dx.doi.org/10.1016/j.virusres.2012.11.004 |
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author | Chan, Chi Ngai McMonagle, Elizabeth L. Hosie, Margaret J. Willett, Brian J. |
author_facet | Chan, Chi Ngai McMonagle, Elizabeth L. Hosie, Margaret J. Willett, Brian J. |
author_sort | Chan, Chi Ngai |
collection | PubMed |
description | The phorbol ester Prostratin may either stimulate or inhibit human immunodeficiency virus-1 (HIV-1) replication. Here we report that Prostratin also exhibits a similar dual action upon feline immunodeficiency virus (FIV) replication in an IL-2-dependent feline CD4(+) T-cell line (MYA-1). While withdrawal of IL-2 halted FIV spread, Prostratin rescued virus production and cell viability, mimicking the functions of the cytokine. Conversely, FIV grew rapidly in the presence of IL-2 and this was inhibited by Prostratin. In contrast to HIV-1, Prostratin mediated inhibition of FIV through means other than blocking virus entry. Co-application of the protein kinase C (PKC) inhibitor Gö6850 with Prostratin reversed both the inhibitory and stimulatory effects, suggesting that PKC is crucial for FIV replication. |
format | Online Article Text |
id | pubmed-3566544 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35665442013-02-07 Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells Chan, Chi Ngai McMonagle, Elizabeth L. Hosie, Margaret J. Willett, Brian J. Virus Res Article The phorbol ester Prostratin may either stimulate or inhibit human immunodeficiency virus-1 (HIV-1) replication. Here we report that Prostratin also exhibits a similar dual action upon feline immunodeficiency virus (FIV) replication in an IL-2-dependent feline CD4(+) T-cell line (MYA-1). While withdrawal of IL-2 halted FIV spread, Prostratin rescued virus production and cell viability, mimicking the functions of the cytokine. Conversely, FIV grew rapidly in the presence of IL-2 and this was inhibited by Prostratin. In contrast to HIV-1, Prostratin mediated inhibition of FIV through means other than blocking virus entry. Co-application of the protein kinase C (PKC) inhibitor Gö6850 with Prostratin reversed both the inhibitory and stimulatory effects, suggesting that PKC is crucial for FIV replication. Elsevier Science 2013-01 /pmc/articles/PMC3566544/ /pubmed/23201205 http://dx.doi.org/10.1016/j.virusres.2012.11.004 Text en © 2013 Elsevier B.V. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Article Chan, Chi Ngai McMonagle, Elizabeth L. Hosie, Margaret J. Willett, Brian J. Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells |
title | Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells |
title_full | Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells |
title_fullStr | Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells |
title_full_unstemmed | Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells |
title_short | Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells |
title_sort | prostratin exhibits both replication enhancing and inhibiting effects on fiv infection of feline cd4(+) t-cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566544/ https://www.ncbi.nlm.nih.gov/pubmed/23201205 http://dx.doi.org/10.1016/j.virusres.2012.11.004 |
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