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Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells

The phorbol ester Prostratin may either stimulate or inhibit human immunodeficiency virus-1 (HIV-1) replication. Here we report that Prostratin also exhibits a similar dual action upon feline immunodeficiency virus (FIV) replication in an IL-2-dependent feline CD4(+) T-cell line (MYA-1). While withd...

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Autores principales: Chan, Chi Ngai, McMonagle, Elizabeth L., Hosie, Margaret J., Willett, Brian J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566544/
https://www.ncbi.nlm.nih.gov/pubmed/23201205
http://dx.doi.org/10.1016/j.virusres.2012.11.004
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author Chan, Chi Ngai
McMonagle, Elizabeth L.
Hosie, Margaret J.
Willett, Brian J.
author_facet Chan, Chi Ngai
McMonagle, Elizabeth L.
Hosie, Margaret J.
Willett, Brian J.
author_sort Chan, Chi Ngai
collection PubMed
description The phorbol ester Prostratin may either stimulate or inhibit human immunodeficiency virus-1 (HIV-1) replication. Here we report that Prostratin also exhibits a similar dual action upon feline immunodeficiency virus (FIV) replication in an IL-2-dependent feline CD4(+) T-cell line (MYA-1). While withdrawal of IL-2 halted FIV spread, Prostratin rescued virus production and cell viability, mimicking the functions of the cytokine. Conversely, FIV grew rapidly in the presence of IL-2 and this was inhibited by Prostratin. In contrast to HIV-1, Prostratin mediated inhibition of FIV through means other than blocking virus entry. Co-application of the protein kinase C (PKC) inhibitor Gö6850 with Prostratin reversed both the inhibitory and stimulatory effects, suggesting that PKC is crucial for FIV replication.
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spelling pubmed-35665442013-02-07 Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells Chan, Chi Ngai McMonagle, Elizabeth L. Hosie, Margaret J. Willett, Brian J. Virus Res Article The phorbol ester Prostratin may either stimulate or inhibit human immunodeficiency virus-1 (HIV-1) replication. Here we report that Prostratin also exhibits a similar dual action upon feline immunodeficiency virus (FIV) replication in an IL-2-dependent feline CD4(+) T-cell line (MYA-1). While withdrawal of IL-2 halted FIV spread, Prostratin rescued virus production and cell viability, mimicking the functions of the cytokine. Conversely, FIV grew rapidly in the presence of IL-2 and this was inhibited by Prostratin. In contrast to HIV-1, Prostratin mediated inhibition of FIV through means other than blocking virus entry. Co-application of the protein kinase C (PKC) inhibitor Gö6850 with Prostratin reversed both the inhibitory and stimulatory effects, suggesting that PKC is crucial for FIV replication. Elsevier Science 2013-01 /pmc/articles/PMC3566544/ /pubmed/23201205 http://dx.doi.org/10.1016/j.virusres.2012.11.004 Text en © 2013 Elsevier B.V. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Chan, Chi Ngai
McMonagle, Elizabeth L.
Hosie, Margaret J.
Willett, Brian J.
Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells
title Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells
title_full Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells
title_fullStr Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells
title_full_unstemmed Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells
title_short Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4(+) T-cells
title_sort prostratin exhibits both replication enhancing and inhibiting effects on fiv infection of feline cd4(+) t-cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566544/
https://www.ncbi.nlm.nih.gov/pubmed/23201205
http://dx.doi.org/10.1016/j.virusres.2012.11.004
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