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Controlled deposition and combing of DNA across lithographically defined patterns on silicon
We have developed a new procedure for efficient combing of DNA on a silicon substrate, which allows reproducible deposition and alignment of DNA molecules across lithographically defined patterns. The technique involves surface modification of Si/SiO(2) substrates with a hydrophobic silane by using...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Beilstein-Institut
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566866/ https://www.ncbi.nlm.nih.gov/pubmed/23399926 http://dx.doi.org/10.3762/bjnano.4.8 |
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author | Esmail Nazari, Zeinab Gurevich, Leonid |
author_facet | Esmail Nazari, Zeinab Gurevich, Leonid |
author_sort | Esmail Nazari, Zeinab |
collection | PubMed |
description | We have developed a new procedure for efficient combing of DNA on a silicon substrate, which allows reproducible deposition and alignment of DNA molecules across lithographically defined patterns. The technique involves surface modification of Si/SiO(2) substrates with a hydrophobic silane by using gas-phase deposition. Thereafter, DNA molecules are aligned by dragging the droplet on the hydrophobic substrate with a pipette tip. Using this procedure, DNA molecules were stretched to an average value of 122% of their contour length. Furthermore, we demonstrated combing of ca. 900 nm long stretches of genomic DNA across nanofabricated electrodes, which was not possible by using other available combing methods. Similar results were also obtained for DNA–peptide conjugates. We suggest this method as a simple yet reliable technique for depositing and aligning DNA and DNA derivatives across nanofabricated patterns. |
format | Online Article Text |
id | pubmed-3566866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Beilstein-Institut |
record_format | MEDLINE/PubMed |
spelling | pubmed-35668662013-02-11 Controlled deposition and combing of DNA across lithographically defined patterns on silicon Esmail Nazari, Zeinab Gurevich, Leonid Beilstein J Nanotechnol Letter We have developed a new procedure for efficient combing of DNA on a silicon substrate, which allows reproducible deposition and alignment of DNA molecules across lithographically defined patterns. The technique involves surface modification of Si/SiO(2) substrates with a hydrophobic silane by using gas-phase deposition. Thereafter, DNA molecules are aligned by dragging the droplet on the hydrophobic substrate with a pipette tip. Using this procedure, DNA molecules were stretched to an average value of 122% of their contour length. Furthermore, we demonstrated combing of ca. 900 nm long stretches of genomic DNA across nanofabricated electrodes, which was not possible by using other available combing methods. Similar results were also obtained for DNA–peptide conjugates. We suggest this method as a simple yet reliable technique for depositing and aligning DNA and DNA derivatives across nanofabricated patterns. Beilstein-Institut 2013-01-31 /pmc/articles/PMC3566866/ /pubmed/23399926 http://dx.doi.org/10.3762/bjnano.4.8 Text en Copyright © 2013, Esmail Nazari and Gurevich https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjnano/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Nanotechnology terms and conditions: (https://www.beilstein-journals.org/bjnano/terms) |
spellingShingle | Letter Esmail Nazari, Zeinab Gurevich, Leonid Controlled deposition and combing of DNA across lithographically defined patterns on silicon |
title | Controlled deposition and combing of DNA across lithographically defined patterns on silicon |
title_full | Controlled deposition and combing of DNA across lithographically defined patterns on silicon |
title_fullStr | Controlled deposition and combing of DNA across lithographically defined patterns on silicon |
title_full_unstemmed | Controlled deposition and combing of DNA across lithographically defined patterns on silicon |
title_short | Controlled deposition and combing of DNA across lithographically defined patterns on silicon |
title_sort | controlled deposition and combing of dna across lithographically defined patterns on silicon |
topic | Letter |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3566866/ https://www.ncbi.nlm.nih.gov/pubmed/23399926 http://dx.doi.org/10.3762/bjnano.4.8 |
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