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Chronic Over-Expression of Heat Shock Protein 27 Attenuates Atherogenesis and Enhances Plaque Remodeling: A Combined Histological and Mechanical Assessment of Aortic Lesions
AIMS: Expression of Heat Shock Protein-27 (HSP27) is reduced in human coronary atherosclerosis. Over-expression of HSP27 is protective against the early formation of lesions in atherosclerosis-prone apoE(−/−) mice (apoE(−/−)HSP27(o/e)) - however, only in females. We now seek to determine if chronic...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3567023/ https://www.ncbi.nlm.nih.gov/pubmed/23409070 http://dx.doi.org/10.1371/journal.pone.0055867 |
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author | Cuerrier, Charles M. Chen, Yong-Xiang Tremblay, Dominique Rayner, Katey McNulty, Melissa Zhao, XiaoLing Kennedy, Christopher R. J. de BelleRoche, Jacqueline Pelling, Andrew E. O'Brien, Edward R. |
author_facet | Cuerrier, Charles M. Chen, Yong-Xiang Tremblay, Dominique Rayner, Katey McNulty, Melissa Zhao, XiaoLing Kennedy, Christopher R. J. de BelleRoche, Jacqueline Pelling, Andrew E. O'Brien, Edward R. |
author_sort | Cuerrier, Charles M. |
collection | PubMed |
description | AIMS: Expression of Heat Shock Protein-27 (HSP27) is reduced in human coronary atherosclerosis. Over-expression of HSP27 is protective against the early formation of lesions in atherosclerosis-prone apoE(−/−) mice (apoE(−/−)HSP27(o/e)) - however, only in females. We now seek to determine if chronic HSP27 over-expression is protective in a model of advanced atherosclerosis in both male and female apoE(−/−) mice. METHODS AND RESULTS: After 12 weeks on a high fat diet, serum HSP27 levels rose more than 16-fold in male and female apoE(−/−)HSP27(o/e) mice, although females had higher levels than males. Relative to apoE(−/−) mice, female apoE(−/−)HSP27(o/e) mice showed reductions in aortic lesion area of 35% for en face and 30% for cross-sectional sinus tissue sections – with the same parameters reduced by 21% and 24% in male cohorts; respectively. Aortic plaques from apoE(−/−)HSP27(o/e) mice showed almost 50% reductions in the area occupied by cholesterol clefts and free cholesterol, with fewer macrophages and reduced apoptosis but greater intimal smooth muscle cell and collagen content. The analysis of the aortic mechanical properties showed increased vessel stiffness in apoE(−/−)HSP27(o/e) mice (41% in female, 34% in male) compare to apoE(−/−) counterparts. CONCLUSIONS: Chronic over-expression of HSP27 is atheroprotective in both sexes and coincides with reductions in lesion cholesterol accumulation as well as favorable plaque remodeling. These data provide new clues as to how HSP27 may improve not only the composition of atherosclerotic lesions but potentially their stability and resilience to plaque rupture. |
format | Online Article Text |
id | pubmed-3567023 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-35670232013-02-13 Chronic Over-Expression of Heat Shock Protein 27 Attenuates Atherogenesis and Enhances Plaque Remodeling: A Combined Histological and Mechanical Assessment of Aortic Lesions Cuerrier, Charles M. Chen, Yong-Xiang Tremblay, Dominique Rayner, Katey McNulty, Melissa Zhao, XiaoLing Kennedy, Christopher R. J. de BelleRoche, Jacqueline Pelling, Andrew E. O'Brien, Edward R. PLoS One Research Article AIMS: Expression of Heat Shock Protein-27 (HSP27) is reduced in human coronary atherosclerosis. Over-expression of HSP27 is protective against the early formation of lesions in atherosclerosis-prone apoE(−/−) mice (apoE(−/−)HSP27(o/e)) - however, only in females. We now seek to determine if chronic HSP27 over-expression is protective in a model of advanced atherosclerosis in both male and female apoE(−/−) mice. METHODS AND RESULTS: After 12 weeks on a high fat diet, serum HSP27 levels rose more than 16-fold in male and female apoE(−/−)HSP27(o/e) mice, although females had higher levels than males. Relative to apoE(−/−) mice, female apoE(−/−)HSP27(o/e) mice showed reductions in aortic lesion area of 35% for en face and 30% for cross-sectional sinus tissue sections – with the same parameters reduced by 21% and 24% in male cohorts; respectively. Aortic plaques from apoE(−/−)HSP27(o/e) mice showed almost 50% reductions in the area occupied by cholesterol clefts and free cholesterol, with fewer macrophages and reduced apoptosis but greater intimal smooth muscle cell and collagen content. The analysis of the aortic mechanical properties showed increased vessel stiffness in apoE(−/−)HSP27(o/e) mice (41% in female, 34% in male) compare to apoE(−/−) counterparts. CONCLUSIONS: Chronic over-expression of HSP27 is atheroprotective in both sexes and coincides with reductions in lesion cholesterol accumulation as well as favorable plaque remodeling. These data provide new clues as to how HSP27 may improve not only the composition of atherosclerotic lesions but potentially their stability and resilience to plaque rupture. Public Library of Science 2013-02-07 /pmc/articles/PMC3567023/ /pubmed/23409070 http://dx.doi.org/10.1371/journal.pone.0055867 Text en © 2013 Cuerrier et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Cuerrier, Charles M. Chen, Yong-Xiang Tremblay, Dominique Rayner, Katey McNulty, Melissa Zhao, XiaoLing Kennedy, Christopher R. J. de BelleRoche, Jacqueline Pelling, Andrew E. O'Brien, Edward R. Chronic Over-Expression of Heat Shock Protein 27 Attenuates Atherogenesis and Enhances Plaque Remodeling: A Combined Histological and Mechanical Assessment of Aortic Lesions |
title | Chronic Over-Expression of Heat Shock Protein 27 Attenuates Atherogenesis and Enhances Plaque Remodeling: A Combined Histological and Mechanical Assessment of Aortic Lesions |
title_full | Chronic Over-Expression of Heat Shock Protein 27 Attenuates Atherogenesis and Enhances Plaque Remodeling: A Combined Histological and Mechanical Assessment of Aortic Lesions |
title_fullStr | Chronic Over-Expression of Heat Shock Protein 27 Attenuates Atherogenesis and Enhances Plaque Remodeling: A Combined Histological and Mechanical Assessment of Aortic Lesions |
title_full_unstemmed | Chronic Over-Expression of Heat Shock Protein 27 Attenuates Atherogenesis and Enhances Plaque Remodeling: A Combined Histological and Mechanical Assessment of Aortic Lesions |
title_short | Chronic Over-Expression of Heat Shock Protein 27 Attenuates Atherogenesis and Enhances Plaque Remodeling: A Combined Histological and Mechanical Assessment of Aortic Lesions |
title_sort | chronic over-expression of heat shock protein 27 attenuates atherogenesis and enhances plaque remodeling: a combined histological and mechanical assessment of aortic lesions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3567023/ https://www.ncbi.nlm.nih.gov/pubmed/23409070 http://dx.doi.org/10.1371/journal.pone.0055867 |
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