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Using Two Dyes with the Same Fluorophore to Monitor Cellular Calcium Concentration in an Extended Range
We extend the sensitivity of quantitative concentration imaging to an approximately 1000-fold range of concentrations by a method that uses two fluorescent dyes with the same fluorophore, having different affinity for the monitored species. While the formulation and illustration refer to a monitor o...
Autores principales: | Figueroa, Lourdes, Shkryl, Vyacheslav M., Blatter, Lothar A., Ríos, Eduardo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3567097/ https://www.ncbi.nlm.nih.gov/pubmed/23409040 http://dx.doi.org/10.1371/journal.pone.0055778 |
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