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In vivo imaging of the rodent eye with swept source/Fourier domain OCT

Swept source/Fourier domain OCT is demonstrated for in vivo imaging of the rodent eye. Using commercial swept laser technology, we developed a prototype OCT imaging system for small animal ocular imaging operating in the 1050 nm wavelength range at an axial scan rate of 100 kHz with ~6 µm axial reso...

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Autores principales: Liu, Jonathan J., Grulkowski, Ireneusz, Kraus, Martin F., Potsaid, Benjamin, Lu, Chen D., Baumann, Bernhard, Duker, Jay S., Hornegger, Joachim, Fujimoto, James G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3567721/
https://www.ncbi.nlm.nih.gov/pubmed/23412778
http://dx.doi.org/10.1364/BOE.4.000351
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author Liu, Jonathan J.
Grulkowski, Ireneusz
Kraus, Martin F.
Potsaid, Benjamin
Lu, Chen D.
Baumann, Bernhard
Duker, Jay S.
Hornegger, Joachim
Fujimoto, James G.
author_facet Liu, Jonathan J.
Grulkowski, Ireneusz
Kraus, Martin F.
Potsaid, Benjamin
Lu, Chen D.
Baumann, Bernhard
Duker, Jay S.
Hornegger, Joachim
Fujimoto, James G.
author_sort Liu, Jonathan J.
collection PubMed
description Swept source/Fourier domain OCT is demonstrated for in vivo imaging of the rodent eye. Using commercial swept laser technology, we developed a prototype OCT imaging system for small animal ocular imaging operating in the 1050 nm wavelength range at an axial scan rate of 100 kHz with ~6 µm axial resolution. The high imaging speed enables volumetric imaging with high axial scan densities, measuring high flow velocities in vessels, and repeated volumetric imaging over time. The 1050 nm wavelength light provides increased penetration into tissue compared to standard commercial OCT systems at 850 nm. The long imaging range enables multiple operating modes for imaging the retina, posterior eye, as well as anterior eye and full eye length. A registration algorithm using orthogonally scanned OCT volumetric data sets which can correct motion on a per A-scan basis is applied to compensate motion and merge motion corrected volumetric data for enhanced OCT image quality. Ultrahigh speed swept source OCT is a promising technique for imaging the rodent eye, proving comprehensive information on the cornea, anterior segment, lens, vitreous, posterior segment, retina and choroid.
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spelling pubmed-35677212013-02-14 In vivo imaging of the rodent eye with swept source/Fourier domain OCT Liu, Jonathan J. Grulkowski, Ireneusz Kraus, Martin F. Potsaid, Benjamin Lu, Chen D. Baumann, Bernhard Duker, Jay S. Hornegger, Joachim Fujimoto, James G. Biomed Opt Express Ophthalmology Applications Swept source/Fourier domain OCT is demonstrated for in vivo imaging of the rodent eye. Using commercial swept laser technology, we developed a prototype OCT imaging system for small animal ocular imaging operating in the 1050 nm wavelength range at an axial scan rate of 100 kHz with ~6 µm axial resolution. The high imaging speed enables volumetric imaging with high axial scan densities, measuring high flow velocities in vessels, and repeated volumetric imaging over time. The 1050 nm wavelength light provides increased penetration into tissue compared to standard commercial OCT systems at 850 nm. The long imaging range enables multiple operating modes for imaging the retina, posterior eye, as well as anterior eye and full eye length. A registration algorithm using orthogonally scanned OCT volumetric data sets which can correct motion on a per A-scan basis is applied to compensate motion and merge motion corrected volumetric data for enhanced OCT image quality. Ultrahigh speed swept source OCT is a promising technique for imaging the rodent eye, proving comprehensive information on the cornea, anterior segment, lens, vitreous, posterior segment, retina and choroid. Optical Society of America 2013-01-29 /pmc/articles/PMC3567721/ /pubmed/23412778 http://dx.doi.org/10.1364/BOE.4.000351 Text en ©2013 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Ophthalmology Applications
Liu, Jonathan J.
Grulkowski, Ireneusz
Kraus, Martin F.
Potsaid, Benjamin
Lu, Chen D.
Baumann, Bernhard
Duker, Jay S.
Hornegger, Joachim
Fujimoto, James G.
In vivo imaging of the rodent eye with swept source/Fourier domain OCT
title In vivo imaging of the rodent eye with swept source/Fourier domain OCT
title_full In vivo imaging of the rodent eye with swept source/Fourier domain OCT
title_fullStr In vivo imaging of the rodent eye with swept source/Fourier domain OCT
title_full_unstemmed In vivo imaging of the rodent eye with swept source/Fourier domain OCT
title_short In vivo imaging of the rodent eye with swept source/Fourier domain OCT
title_sort in vivo imaging of the rodent eye with swept source/fourier domain oct
topic Ophthalmology Applications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3567721/
https://www.ncbi.nlm.nih.gov/pubmed/23412778
http://dx.doi.org/10.1364/BOE.4.000351
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