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Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice
The present study was performed to investigate the effects of the combination therapy of pinocembrin and simvastatin on the atherosclerotic lesions development in the ApoE(−/−) mice. METHODS: Eight-week-old male ApoE(−/−) mice were fed high fat diet (HFD) and treated with simvastatin (10 mg/kg per d...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3567959/ https://www.ncbi.nlm.nih.gov/pubmed/23216643 http://dx.doi.org/10.1186/1476-511X-11-166 |
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author | Sang, Hui Yuan, Na Yao, Shutong Li, Furong Wang, Jiafu Fang, Yongqi Qin, Shucun |
author_facet | Sang, Hui Yuan, Na Yao, Shutong Li, Furong Wang, Jiafu Fang, Yongqi Qin, Shucun |
author_sort | Sang, Hui |
collection | PubMed |
description | The present study was performed to investigate the effects of the combination therapy of pinocembrin and simvastatin on the atherosclerotic lesions development in the ApoE(−/−) mice. METHODS: Eight-week-old male ApoE(−/−) mice were fed high fat diet (HFD) and treated with simvastatin (10 mg/kg per day), pinocembrin (20 mg/kg per day), or the combination therapy (simvastatin 5 mg/kg per day and pinocembrin 20 mg/kg per day) for 14 weeks. The serum lipid levels, nitric oxide (NO), endothelin (ET), superoxide dismutase (SOD) and malondialdehyde (MDA) were determined with spectrophotometric measurement and ELISA assay. Vascular endothelial growth factor (VEGF) in serum and aortic root was detected. En face analyses of atherosclerotic lesion in whole aorta and aortic root sections were performed with plaque staining using oil red O. RESULTS: The combination treatment with simvastatin and pinocembrin resulted in significantly decreased levels of serum total cholesterol, triglycerides and low-density lipoprotein cholesterol, augmented NO levels and SOD activity, inhibited ET and VEGF expression. Immunohistochemistry of aortic valve sections revealed that the combination therapy also suppressed the expression of VEGF induced by HFD. In addition, HFD-induced arterial wall lipid disposition displayed by oil red O staining was reduced significantly in aortic root and whole aorta en face in the combination administrated mice. The effect of the combination was superior to simvastatin alone. CONCLUSION: The combination of simvastatin and pinocembrin synergistically inhibited atherosclerotic lesion development in ApoE(−/−) mice with hyperlipidemia, which is partially dependent on the protective of vascular endothelium. |
format | Online Article Text |
id | pubmed-3567959 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-35679592013-02-12 Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice Sang, Hui Yuan, Na Yao, Shutong Li, Furong Wang, Jiafu Fang, Yongqi Qin, Shucun Lipids Health Dis Research The present study was performed to investigate the effects of the combination therapy of pinocembrin and simvastatin on the atherosclerotic lesions development in the ApoE(−/−) mice. METHODS: Eight-week-old male ApoE(−/−) mice were fed high fat diet (HFD) and treated with simvastatin (10 mg/kg per day), pinocembrin (20 mg/kg per day), or the combination therapy (simvastatin 5 mg/kg per day and pinocembrin 20 mg/kg per day) for 14 weeks. The serum lipid levels, nitric oxide (NO), endothelin (ET), superoxide dismutase (SOD) and malondialdehyde (MDA) were determined with spectrophotometric measurement and ELISA assay. Vascular endothelial growth factor (VEGF) in serum and aortic root was detected. En face analyses of atherosclerotic lesion in whole aorta and aortic root sections were performed with plaque staining using oil red O. RESULTS: The combination treatment with simvastatin and pinocembrin resulted in significantly decreased levels of serum total cholesterol, triglycerides and low-density lipoprotein cholesterol, augmented NO levels and SOD activity, inhibited ET and VEGF expression. Immunohistochemistry of aortic valve sections revealed that the combination therapy also suppressed the expression of VEGF induced by HFD. In addition, HFD-induced arterial wall lipid disposition displayed by oil red O staining was reduced significantly in aortic root and whole aorta en face in the combination administrated mice. The effect of the combination was superior to simvastatin alone. CONCLUSION: The combination of simvastatin and pinocembrin synergistically inhibited atherosclerotic lesion development in ApoE(−/−) mice with hyperlipidemia, which is partially dependent on the protective of vascular endothelium. BioMed Central 2012-12-05 /pmc/articles/PMC3567959/ /pubmed/23216643 http://dx.doi.org/10.1186/1476-511X-11-166 Text en Copyright ©2012 Sang et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Sang, Hui Yuan, Na Yao, Shutong Li, Furong Wang, Jiafu Fang, Yongqi Qin, Shucun Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice |
title | Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice |
title_full | Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice |
title_fullStr | Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice |
title_full_unstemmed | Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice |
title_short | Inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoE-deficient mice |
title_sort | inhibitory effect of the combination therapy of simvastatin and pinocembrin on atherosclerosis in apoe-deficient mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3567959/ https://www.ncbi.nlm.nih.gov/pubmed/23216643 http://dx.doi.org/10.1186/1476-511X-11-166 |
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