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Recombinant Production of Human Aquaporin-1 to an Exceptional High Membrane Density in Saccharomyces cerevisiae

In the present paper we explored the capacity of yeast Saccharomyces cerevisiae as host for heterologous expression of human Aquaporin-1. Aquaporin-1 cDNA was expressed from a galactose inducible promoter situated on a plasmid with an adjustable copy number. Human Aquaporin-1 was C-terminally tagged...

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Detalles Bibliográficos
Autores principales:	Bomholt, Julie, Hélix-Nielsen, Claus, Scharff-Poulsen, Peter, Pedersen, Per Amstrup
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569440/ https://www.ncbi.nlm.nih.gov/pubmed/23409185 http://dx.doi.org/10.1371/journal.pone.0056431

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