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Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis
Bovine tuberculosis is an important infectious disease caused by Mycobacterium bovis, which is responsible for considerable economic losses. This disease constitutes a serious public health problem. Control programs in most countries, including Brazil, are based on the identification and slaughter o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing AG
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569591/ https://www.ncbi.nlm.nih.gov/pubmed/23419946 http://dx.doi.org/10.1186/2193-1801-1-77 |
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author | Souza, Ingrid IF Melo, Elaine SP Ramos, Carlos AN Farias, Thaís A Osório, Ana Luiza AR Jorge, Klaudia SG Vidal, Carlos ES Silva, Altino S Silva, Márcio R Pellegrin, Aiesca O Araújo, Flábio R |
author_facet | Souza, Ingrid IF Melo, Elaine SP Ramos, Carlos AN Farias, Thaís A Osório, Ana Luiza AR Jorge, Klaudia SG Vidal, Carlos ES Silva, Altino S Silva, Márcio R Pellegrin, Aiesca O Araújo, Flábio R |
author_sort | Souza, Ingrid IF |
collection | PubMed |
description | Bovine tuberculosis is an important infectious disease caused by Mycobacterium bovis, which is responsible for considerable economic losses. This disease constitutes a serious public health problem. Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints. In the present study, the recombinant proteins CFP-10, ESAT-6, Mb0143, MPB83, PE5, PE13, TB10.4, TB15.3 and a chimera of ESAT-6/MPB70/MPB83 (fusion protein) were tested as ELISA antigens for the diagnosis of bovine tuberculosis. The proteins were produced in Escherichia coli, purified and tested in ELISAs with sera from 126 cattle having tested negative in the comparative intradermal tuberculin test (CITT) and 107 sera from cattle having tested positive in the CITT. Also, 236 sera from two BTB-free beef cattle herds were tested. Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity. The ELISA absorbances of the cattle sera from BTB-free herds showed similar levels to those of CITT positive cattle, probably as the result of successive skin tuberculinizations to define the BTB-free status of the herds. However, the ELISA with the ESAT-6/MPB70/MPB83 chimera was useful to discriminate BTB positive and negative cattle in herds prior to the tuberculin skin test. |
format | Online Article Text |
id | pubmed-3569591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer International Publishing AG |
record_format | MEDLINE/PubMed |
spelling | pubmed-35695912013-02-14 Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis Souza, Ingrid IF Melo, Elaine SP Ramos, Carlos AN Farias, Thaís A Osório, Ana Luiza AR Jorge, Klaudia SG Vidal, Carlos ES Silva, Altino S Silva, Márcio R Pellegrin, Aiesca O Araújo, Flábio R Springerplus Research Bovine tuberculosis is an important infectious disease caused by Mycobacterium bovis, which is responsible for considerable economic losses. This disease constitutes a serious public health problem. Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints. In the present study, the recombinant proteins CFP-10, ESAT-6, Mb0143, MPB83, PE5, PE13, TB10.4, TB15.3 and a chimera of ESAT-6/MPB70/MPB83 (fusion protein) were tested as ELISA antigens for the diagnosis of bovine tuberculosis. The proteins were produced in Escherichia coli, purified and tested in ELISAs with sera from 126 cattle having tested negative in the comparative intradermal tuberculin test (CITT) and 107 sera from cattle having tested positive in the CITT. Also, 236 sera from two BTB-free beef cattle herds were tested. Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity. The ELISA absorbances of the cattle sera from BTB-free herds showed similar levels to those of CITT positive cattle, probably as the result of successive skin tuberculinizations to define the BTB-free status of the herds. However, the ELISA with the ESAT-6/MPB70/MPB83 chimera was useful to discriminate BTB positive and negative cattle in herds prior to the tuberculin skin test. Springer International Publishing AG 2012-12-22 /pmc/articles/PMC3569591/ /pubmed/23419946 http://dx.doi.org/10.1186/2193-1801-1-77 Text en © Souza et al.; licensee Springer. 2012 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Souza, Ingrid IF Melo, Elaine SP Ramos, Carlos AN Farias, Thaís A Osório, Ana Luiza AR Jorge, Klaudia SG Vidal, Carlos ES Silva, Altino S Silva, Márcio R Pellegrin, Aiesca O Araújo, Flábio R Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis |
title | Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis |
title_full | Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis |
title_fullStr | Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis |
title_full_unstemmed | Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis |
title_short | Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis |
title_sort | screening of recombinant proteins as antigens in indirect elisa for diagnosis of bovine tuberculosis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569591/ https://www.ncbi.nlm.nih.gov/pubmed/23419946 http://dx.doi.org/10.1186/2193-1801-1-77 |
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