The Complex of Cytochrome f and Plastocyanin from Nostoc sp. PCC 7119 is Highly Dynamic

Cytochrome f (Cyt f) and plastocyanin (Pc) form a highly transient complex as part of the photosynthetic redox chain. The complex from Nostoc sp. PCC 7119 was studied by NMR relaxation spectroscopy with the aim of determining the orientation of Pc relative to Cyt f. Chemical-shift-perturbation analy...

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Autores principales: Scanu, Sandra, Förster, Johannes, Finiguerra, Michela G, Shabestari, Maryam Hashemi, Huber, Martina, Ubbink, Marcellus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2012
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569876/
https://www.ncbi.nlm.nih.gov/pubmed/22619165
http://dx.doi.org/10.1002/cbic.201200073
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author Scanu, Sandra
Förster, Johannes
Finiguerra, Michela G
Shabestari, Maryam Hashemi
Huber, Martina
Ubbink, Marcellus
author_facet Scanu, Sandra
Förster, Johannes
Finiguerra, Michela G
Shabestari, Maryam Hashemi
Huber, Martina
Ubbink, Marcellus
author_sort Scanu, Sandra
collection PubMed
description Cytochrome f (Cyt f) and plastocyanin (Pc) form a highly transient complex as part of the photosynthetic redox chain. The complex from Nostoc sp. PCC 7119 was studied by NMR relaxation spectroscopy with the aim of determining the orientation of Pc relative to Cyt f. Chemical-shift-perturbation analysis showed that the presence of spin labels on the surface of Cyt f does not significantly affect the binding of Pc. The paramagnetic relaxation enhancement results are not consistent with a single orientation of Pc, thus indicating that multiple orientations must occur and suggesting that an encounter state represents a large fraction of the complex.
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spelling pubmed-35698762013-03-05 The Complex of Cytochrome f and Plastocyanin from Nostoc sp. PCC 7119 is Highly Dynamic Scanu, Sandra Förster, Johannes Finiguerra, Michela G Shabestari, Maryam Hashemi Huber, Martina Ubbink, Marcellus Chembiochem Full Papers Cytochrome f (Cyt f) and plastocyanin (Pc) form a highly transient complex as part of the photosynthetic redox chain. The complex from Nostoc sp. PCC 7119 was studied by NMR relaxation spectroscopy with the aim of determining the orientation of Pc relative to Cyt f. Chemical-shift-perturbation analysis showed that the presence of spin labels on the surface of Cyt f does not significantly affect the binding of Pc. The paramagnetic relaxation enhancement results are not consistent with a single orientation of Pc, thus indicating that multiple orientations must occur and suggesting that an encounter state represents a large fraction of the complex. WILEY-VCH Verlag 2012-06-18 2012-05-22 /pmc/articles/PMC3569876/ /pubmed/22619165 http://dx.doi.org/10.1002/cbic.201200073 Text en Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Full Papers
Scanu, Sandra
Förster, Johannes
Finiguerra, Michela G
Shabestari, Maryam Hashemi
Huber, Martina
Ubbink, Marcellus
The Complex of Cytochrome f and Plastocyanin from Nostoc sp. PCC 7119 is Highly Dynamic
title The Complex of Cytochrome f and Plastocyanin from Nostoc sp. PCC 7119 is Highly Dynamic
title_full The Complex of Cytochrome f and Plastocyanin from Nostoc sp. PCC 7119 is Highly Dynamic
title_fullStr The Complex of Cytochrome f and Plastocyanin from Nostoc sp. PCC 7119 is Highly Dynamic
title_full_unstemmed The Complex of Cytochrome f and Plastocyanin from Nostoc sp. PCC 7119 is Highly Dynamic
title_short The Complex of Cytochrome f and Plastocyanin from Nostoc sp. PCC 7119 is Highly Dynamic
title_sort complex of cytochrome f and plastocyanin from nostoc sp. pcc 7119 is highly dynamic
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569876/
https://www.ncbi.nlm.nih.gov/pubmed/22619165
http://dx.doi.org/10.1002/cbic.201200073
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