Cargando…
Adipose Stem Cell Coating of Biomimetic β-TCP Macrospheres by Use of Laboratory Centrifuge
Biomimetic materials such as coral exoskeletons possess unique architectural structures with a uniform and interconnected porous network that can be beneficial as a scaffold material. In addition, these marine structures can be hydrothermally converted to calcium phosphates, while retaining the orig...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mary Ann Liebert, Inc.
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569929/ https://www.ncbi.nlm.nih.gov/pubmed/23515356 http://dx.doi.org/10.1089/biores.2012.0269 |
_version_ | 1782258989453541376 |
---|---|
author | Chou, Joshua Green, David W. Singh, Krishneel Hao, Jia Ben-Nissan, Besim Milthorpe, Bruce |
author_facet | Chou, Joshua Green, David W. Singh, Krishneel Hao, Jia Ben-Nissan, Besim Milthorpe, Bruce |
author_sort | Chou, Joshua |
collection | PubMed |
description | Biomimetic materials such as coral exoskeletons possess unique architectural structures with a uniform and interconnected porous network that can be beneficial as a scaffold material. In addition, these marine structures can be hydrothermally converted to calcium phosphates, while retaining the original structural properties. The ability of biomaterials to stimulate the local microenvironment is one of the main focuses in tissue engineering, and directly coating the scaffold with stem cells facilitates future potential applications in therapeutics and regenerative medicine. In this article we describe a new and simple method that uses a laboratory centrifuge to coat hydrothermally derived beta-tricalcium phosphate macrospheres from coral exoskeleton with stem cells. In this research the optimal seeding duration and speed were determined to be 1 min and 700 g. Scanning electron micrographs showed complete surface coverage by stem cells within 7 days of seeding. This study constitutes an important step toward achieving functional tissue-engineered implants by increasing our understanding of the influence of dynamic parameters on the efficiency and distribution of stem cell attachment to biomimetic materials and how stem cells interact with biomimetic materials. |
format | Online Article Text |
id | pubmed-3569929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Mary Ann Liebert, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-35699292013-03-20 Adipose Stem Cell Coating of Biomimetic β-TCP Macrospheres by Use of Laboratory Centrifuge Chou, Joshua Green, David W. Singh, Krishneel Hao, Jia Ben-Nissan, Besim Milthorpe, Bruce Biores Open Access Technical Reports Biomimetic materials such as coral exoskeletons possess unique architectural structures with a uniform and interconnected porous network that can be beneficial as a scaffold material. In addition, these marine structures can be hydrothermally converted to calcium phosphates, while retaining the original structural properties. The ability of biomaterials to stimulate the local microenvironment is one of the main focuses in tissue engineering, and directly coating the scaffold with stem cells facilitates future potential applications in therapeutics and regenerative medicine. In this article we describe a new and simple method that uses a laboratory centrifuge to coat hydrothermally derived beta-tricalcium phosphate macrospheres from coral exoskeleton with stem cells. In this research the optimal seeding duration and speed were determined to be 1 min and 700 g. Scanning electron micrographs showed complete surface coverage by stem cells within 7 days of seeding. This study constitutes an important step toward achieving functional tissue-engineered implants by increasing our understanding of the influence of dynamic parameters on the efficiency and distribution of stem cell attachment to biomimetic materials and how stem cells interact with biomimetic materials. Mary Ann Liebert, Inc. 2013-02 /pmc/articles/PMC3569929/ /pubmed/23515356 http://dx.doi.org/10.1089/biores.2012.0269 Text en Copyright 2013, Mary Ann Liebert, Inc. |
spellingShingle | Technical Reports Chou, Joshua Green, David W. Singh, Krishneel Hao, Jia Ben-Nissan, Besim Milthorpe, Bruce Adipose Stem Cell Coating of Biomimetic β-TCP Macrospheres by Use of Laboratory Centrifuge |
title | Adipose Stem Cell Coating of Biomimetic β-TCP Macrospheres by Use of Laboratory Centrifuge |
title_full | Adipose Stem Cell Coating of Biomimetic β-TCP Macrospheres by Use of Laboratory Centrifuge |
title_fullStr | Adipose Stem Cell Coating of Biomimetic β-TCP Macrospheres by Use of Laboratory Centrifuge |
title_full_unstemmed | Adipose Stem Cell Coating of Biomimetic β-TCP Macrospheres by Use of Laboratory Centrifuge |
title_short | Adipose Stem Cell Coating of Biomimetic β-TCP Macrospheres by Use of Laboratory Centrifuge |
title_sort | adipose stem cell coating of biomimetic β-tcp macrospheres by use of laboratory centrifuge |
topic | Technical Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569929/ https://www.ncbi.nlm.nih.gov/pubmed/23515356 http://dx.doi.org/10.1089/biores.2012.0269 |
work_keys_str_mv | AT choujoshua adiposestemcellcoatingofbiomimeticbtcpmacrospheresbyuseoflaboratorycentrifuge AT greendavidw adiposestemcellcoatingofbiomimeticbtcpmacrospheresbyuseoflaboratorycentrifuge AT singhkrishneel adiposestemcellcoatingofbiomimeticbtcpmacrospheresbyuseoflaboratorycentrifuge AT haojia adiposestemcellcoatingofbiomimeticbtcpmacrospheresbyuseoflaboratorycentrifuge AT bennissanbesim adiposestemcellcoatingofbiomimeticbtcpmacrospheresbyuseoflaboratorycentrifuge AT milthorpebruce adiposestemcellcoatingofbiomimeticbtcpmacrospheresbyuseoflaboratorycentrifuge |