Cargando…
Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro
Drug combination therapies are common practice in the treatment of cancer. Cisplatin is the most active chemotherapeutic agent for lung cancer treatment. Osthole is a natural compound extracted from a number of medicinal plants. To determine whether osthole enhances the anticancer effect of cisplati...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570148/ https://www.ncbi.nlm.nih.gov/pubmed/23404433 http://dx.doi.org/10.3892/etm.2013.889 |
_version_ | 1782259015972028416 |
---|---|
author | XU, XIAO-MAN ZHANG, YI QU, DAN LIU, HONG-BO GU, XIU JIAO, GUANG-YU ZHAO, LI |
author_facet | XU, XIAO-MAN ZHANG, YI QU, DAN LIU, HONG-BO GU, XIU JIAO, GUANG-YU ZHAO, LI |
author_sort | XU, XIAO-MAN |
collection | PubMed |
description | Drug combination therapies are common practice in the treatment of cancer. Cisplatin is the most active chemotherapeutic agent for lung cancer treatment. Osthole is a natural compound extracted from a number of medicinal plants. To determine whether osthole enhances the anticancer effect of cisplatin in human lung cancer, we treated NCI-H460 cells with osthole alone or in combination with cisplatin and evaluated cell growth and apoptosis using 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry and fluorescence microscopy. The results showed that, in comparison with single agent treatment, the combination of osthole and cisplatin resulted in greater efficacy in growth inhibition and apoptosis induction. Western blot analysis revealed that the combination effect of osthole and cisplatin was due to regulation of the Bcl-2 family proteins. Findings of this investigation suggested that osthole combined with cisplatin is a potential clinical chemotherapeutic approach in human lung cancer. |
format | Online Article Text |
id | pubmed-3570148 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-35701482013-02-12 Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro XU, XIAO-MAN ZHANG, YI QU, DAN LIU, HONG-BO GU, XIU JIAO, GUANG-YU ZHAO, LI Exp Ther Med Articles Drug combination therapies are common practice in the treatment of cancer. Cisplatin is the most active chemotherapeutic agent for lung cancer treatment. Osthole is a natural compound extracted from a number of medicinal plants. To determine whether osthole enhances the anticancer effect of cisplatin in human lung cancer, we treated NCI-H460 cells with osthole alone or in combination with cisplatin and evaluated cell growth and apoptosis using 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry and fluorescence microscopy. The results showed that, in comparison with single agent treatment, the combination of osthole and cisplatin resulted in greater efficacy in growth inhibition and apoptosis induction. Western blot analysis revealed that the combination effect of osthole and cisplatin was due to regulation of the Bcl-2 family proteins. Findings of this investigation suggested that osthole combined with cisplatin is a potential clinical chemotherapeutic approach in human lung cancer. D.A. Spandidos 2013-03 2013-01-08 /pmc/articles/PMC3570148/ /pubmed/23404433 http://dx.doi.org/10.3892/etm.2013.889 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles XU, XIAO-MAN ZHANG, YI QU, DAN LIU, HONG-BO GU, XIU JIAO, GUANG-YU ZHAO, LI Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro |
title | Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro |
title_full | Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro |
title_fullStr | Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro |
title_full_unstemmed | Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro |
title_short | Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro |
title_sort | combined anticancer activity of osthole and cisplatin in nci-h460 lung cancer cells in vitro |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570148/ https://www.ncbi.nlm.nih.gov/pubmed/23404433 http://dx.doi.org/10.3892/etm.2013.889 |
work_keys_str_mv | AT xuxiaoman combinedanticanceractivityofostholeandcisplatininncih460lungcancercellsinvitro AT zhangyi combinedanticanceractivityofostholeandcisplatininncih460lungcancercellsinvitro AT qudan combinedanticanceractivityofostholeandcisplatininncih460lungcancercellsinvitro AT liuhongbo combinedanticanceractivityofostholeandcisplatininncih460lungcancercellsinvitro AT guxiu combinedanticanceractivityofostholeandcisplatininncih460lungcancercellsinvitro AT jiaoguangyu combinedanticanceractivityofostholeandcisplatininncih460lungcancercellsinvitro AT zhaoli combinedanticanceractivityofostholeandcisplatininncih460lungcancercellsinvitro |