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Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro

Drug combination therapies are common practice in the treatment of cancer. Cisplatin is the most active chemotherapeutic agent for lung cancer treatment. Osthole is a natural compound extracted from a number of medicinal plants. To determine whether osthole enhances the anticancer effect of cisplati...

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Autores principales: XU, XIAO-MAN, ZHANG, YI, QU, DAN, LIU, HONG-BO, GU, XIU, JIAO, GUANG-YU, ZHAO, LI
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570148/
https://www.ncbi.nlm.nih.gov/pubmed/23404433
http://dx.doi.org/10.3892/etm.2013.889
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author XU, XIAO-MAN
ZHANG, YI
QU, DAN
LIU, HONG-BO
GU, XIU
JIAO, GUANG-YU
ZHAO, LI
author_facet XU, XIAO-MAN
ZHANG, YI
QU, DAN
LIU, HONG-BO
GU, XIU
JIAO, GUANG-YU
ZHAO, LI
author_sort XU, XIAO-MAN
collection PubMed
description Drug combination therapies are common practice in the treatment of cancer. Cisplatin is the most active chemotherapeutic agent for lung cancer treatment. Osthole is a natural compound extracted from a number of medicinal plants. To determine whether osthole enhances the anticancer effect of cisplatin in human lung cancer, we treated NCI-H460 cells with osthole alone or in combination with cisplatin and evaluated cell growth and apoptosis using 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry and fluorescence microscopy. The results showed that, in comparison with single agent treatment, the combination of osthole and cisplatin resulted in greater efficacy in growth inhibition and apoptosis induction. Western blot analysis revealed that the combination effect of osthole and cisplatin was due to regulation of the Bcl-2 family proteins. Findings of this investigation suggested that osthole combined with cisplatin is a potential clinical chemotherapeutic approach in human lung cancer.
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spelling pubmed-35701482013-02-12 Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro XU, XIAO-MAN ZHANG, YI QU, DAN LIU, HONG-BO GU, XIU JIAO, GUANG-YU ZHAO, LI Exp Ther Med Articles Drug combination therapies are common practice in the treatment of cancer. Cisplatin is the most active chemotherapeutic agent for lung cancer treatment. Osthole is a natural compound extracted from a number of medicinal plants. To determine whether osthole enhances the anticancer effect of cisplatin in human lung cancer, we treated NCI-H460 cells with osthole alone or in combination with cisplatin and evaluated cell growth and apoptosis using 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry and fluorescence microscopy. The results showed that, in comparison with single agent treatment, the combination of osthole and cisplatin resulted in greater efficacy in growth inhibition and apoptosis induction. Western blot analysis revealed that the combination effect of osthole and cisplatin was due to regulation of the Bcl-2 family proteins. Findings of this investigation suggested that osthole combined with cisplatin is a potential clinical chemotherapeutic approach in human lung cancer. D.A. Spandidos 2013-03 2013-01-08 /pmc/articles/PMC3570148/ /pubmed/23404433 http://dx.doi.org/10.3892/etm.2013.889 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
XU, XIAO-MAN
ZHANG, YI
QU, DAN
LIU, HONG-BO
GU, XIU
JIAO, GUANG-YU
ZHAO, LI
Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro
title Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro
title_full Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro
title_fullStr Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro
title_full_unstemmed Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro
title_short Combined anticancer activity of osthole and cisplatin in NCI-H460 lung cancer cells in vitro
title_sort combined anticancer activity of osthole and cisplatin in nci-h460 lung cancer cells in vitro
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570148/
https://www.ncbi.nlm.nih.gov/pubmed/23404433
http://dx.doi.org/10.3892/etm.2013.889
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