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Protective effects of Quercus salicina on alloxan-induced oxidative stress in HIT-T15 pancreatic β cells

The present study was designed to investigate the protective effect of hot water extracts from Quercus salicina leaves (QSWE) on alloxan-induced oxidative stress in HIT-T15 Syrian hamster pancreatic insulinoma cells. The HIT-T15 cells were treated with alloxan (1 mM) for 1 h and then co-incubated wi...

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Autores principales: SONG, JIA-LE, ZHAO, XIN, WANG, QIANG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570244/
https://www.ncbi.nlm.nih.gov/pubmed/23408741
http://dx.doi.org/10.3892/etm.2013.885
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author SONG, JIA-LE
ZHAO, XIN
WANG, QIANG
author_facet SONG, JIA-LE
ZHAO, XIN
WANG, QIANG
author_sort SONG, JIA-LE
collection PubMed
description The present study was designed to investigate the protective effect of hot water extracts from Quercus salicina leaves (QSWE) on alloxan-induced oxidative stress in HIT-T15 Syrian hamster pancreatic insulinoma cells. The HIT-T15 cells were treated with alloxan (1 mM) for 1 h and then co-incubated with the QSWE for 24 h. Alloxan significantly decreased the viability of the HIT-T15 cells (P<0.05). QSWE did not exhibit significantly cytotoxic effects and increased the viability of the HIT-T15 cells in a concentration-dependent manner. To further investigate the protective effects of QSWE on alloxan-induced oxidative stress in HIT-T15 cells, the cellular levels of reactive oxygen species (ROS), lipid peroxidation and endogenous antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), were analyzed. QSWE decreased the intracellular levels of ROS and lipid peroxidation and increased the activity of antioxidant enzymes. These results suggest that QSWE exerted cytoprotective activity against alloxan-induced oxidative stress in HIT-T15 cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzyme activity. In addition, QSWE also increased the insulin secretion activity of the alloxan-treated HIT-T15 cells.
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spelling pubmed-35702442013-02-13 Protective effects of Quercus salicina on alloxan-induced oxidative stress in HIT-T15 pancreatic β cells SONG, JIA-LE ZHAO, XIN WANG, QIANG Exp Ther Med Articles The present study was designed to investigate the protective effect of hot water extracts from Quercus salicina leaves (QSWE) on alloxan-induced oxidative stress in HIT-T15 Syrian hamster pancreatic insulinoma cells. The HIT-T15 cells were treated with alloxan (1 mM) for 1 h and then co-incubated with the QSWE for 24 h. Alloxan significantly decreased the viability of the HIT-T15 cells (P<0.05). QSWE did not exhibit significantly cytotoxic effects and increased the viability of the HIT-T15 cells in a concentration-dependent manner. To further investigate the protective effects of QSWE on alloxan-induced oxidative stress in HIT-T15 cells, the cellular levels of reactive oxygen species (ROS), lipid peroxidation and endogenous antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), were analyzed. QSWE decreased the intracellular levels of ROS and lipid peroxidation and increased the activity of antioxidant enzymes. These results suggest that QSWE exerted cytoprotective activity against alloxan-induced oxidative stress in HIT-T15 cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzyme activity. In addition, QSWE also increased the insulin secretion activity of the alloxan-treated HIT-T15 cells. D.A. Spandidos 2013-03 2013-01-07 /pmc/articles/PMC3570244/ /pubmed/23408741 http://dx.doi.org/10.3892/etm.2013.885 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
SONG, JIA-LE
ZHAO, XIN
WANG, QIANG
Protective effects of Quercus salicina on alloxan-induced oxidative stress in HIT-T15 pancreatic β cells
title Protective effects of Quercus salicina on alloxan-induced oxidative stress in HIT-T15 pancreatic β cells
title_full Protective effects of Quercus salicina on alloxan-induced oxidative stress in HIT-T15 pancreatic β cells
title_fullStr Protective effects of Quercus salicina on alloxan-induced oxidative stress in HIT-T15 pancreatic β cells
title_full_unstemmed Protective effects of Quercus salicina on alloxan-induced oxidative stress in HIT-T15 pancreatic β cells
title_short Protective effects of Quercus salicina on alloxan-induced oxidative stress in HIT-T15 pancreatic β cells
title_sort protective effects of quercus salicina on alloxan-induced oxidative stress in hit-t15 pancreatic β cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570244/
https://www.ncbi.nlm.nih.gov/pubmed/23408741
http://dx.doi.org/10.3892/etm.2013.885
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