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Development and validation of a HPLC-MS/MS method with electrospray ionization for quantitation of potassium oxonate in human plasma: Application to a pharmacokinetic study

A rapid, sensitive and specific HPLC-MS/MS method was developed and validated for the quantification of potassium oxonate (Oxo) in human plasma using [(13)C(2),(15)N(3)]-Oxo as an internal standard (IS). The target substance was separated from human plasma using the solid-phase extraction method. Ch...

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Autores principales: HAO, GUANGTAO, BAI, SHAOBO, LIANG, HAIXIA, LIANG, YUGUANG, QU, HENGYAN, GAO, HONGZHI, LI, YUANYUAN, ZHENG, ZHUANJIE, WANG, XIAOFANG, LIU, ZEYUAN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570248/
https://www.ncbi.nlm.nih.gov/pubmed/23407381
http://dx.doi.org/10.3892/etm.2013.908
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author HAO, GUANGTAO
BAI, SHAOBO
LIANG, HAIXIA
LIANG, YUGUANG
QU, HENGYAN
GAO, HONGZHI
LI, YUANYUAN
ZHENG, ZHUANJIE
WANG, XIAOFANG
LIU, ZEYUAN
author_facet HAO, GUANGTAO
BAI, SHAOBO
LIANG, HAIXIA
LIANG, YUGUANG
QU, HENGYAN
GAO, HONGZHI
LI, YUANYUAN
ZHENG, ZHUANJIE
WANG, XIAOFANG
LIU, ZEYUAN
author_sort HAO, GUANGTAO
collection PubMed
description A rapid, sensitive and specific HPLC-MS/MS method was developed and validated for the quantification of potassium oxonate (Oxo) in human plasma using [(13)C(2),(15)N(3)]-Oxo as an internal standard (IS). The target substance was separated from human plasma using the solid-phase extraction method. Chromatography separation was performed on a Waters:Atlantis dC(18) column (150×4.6 mm, 5.0 μm) with a mobile phase consisting of H(2)O with 0.1% formic acid in acetonitrile (90:10, v/v). The mass spectrometer works with electrospray ionization and multiple reaction monitoring in its negative ion mode, using target ions at [M–H](−) m/z 111.9 for Oxo and [M–H](−) m/z 117.0 for the IS. The mean standard curve was linear (r=0.9991) over the concentration range of 2.0–200.0 ng/ml and had good back-calculated accuracy and precision. The intra- and inter-day precision were <6.33% and the accuracy was >99.38%. The extraction recovery was >60.26%. The lower limit of quantification achieved with this method was 2.0 ng/ml. This assay method was demonstrated to be accurate, sensitive and simple and was successfully applied to a pharmacokinetic study following single oral administration of a 40-mg S-1 capsule in 12 tumor patients.
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spelling pubmed-35702482013-02-13 Development and validation of a HPLC-MS/MS method with electrospray ionization for quantitation of potassium oxonate in human plasma: Application to a pharmacokinetic study HAO, GUANGTAO BAI, SHAOBO LIANG, HAIXIA LIANG, YUGUANG QU, HENGYAN GAO, HONGZHI LI, YUANYUAN ZHENG, ZHUANJIE WANG, XIAOFANG LIU, ZEYUAN Exp Ther Med Articles A rapid, sensitive and specific HPLC-MS/MS method was developed and validated for the quantification of potassium oxonate (Oxo) in human plasma using [(13)C(2),(15)N(3)]-Oxo as an internal standard (IS). The target substance was separated from human plasma using the solid-phase extraction method. Chromatography separation was performed on a Waters:Atlantis dC(18) column (150×4.6 mm, 5.0 μm) with a mobile phase consisting of H(2)O with 0.1% formic acid in acetonitrile (90:10, v/v). The mass spectrometer works with electrospray ionization and multiple reaction monitoring in its negative ion mode, using target ions at [M–H](−) m/z 111.9 for Oxo and [M–H](−) m/z 117.0 for the IS. The mean standard curve was linear (r=0.9991) over the concentration range of 2.0–200.0 ng/ml and had good back-calculated accuracy and precision. The intra- and inter-day precision were <6.33% and the accuracy was >99.38%. The extraction recovery was >60.26%. The lower limit of quantification achieved with this method was 2.0 ng/ml. This assay method was demonstrated to be accurate, sensitive and simple and was successfully applied to a pharmacokinetic study following single oral administration of a 40-mg S-1 capsule in 12 tumor patients. D.A. Spandidos 2013-03 2013-01-18 /pmc/articles/PMC3570248/ /pubmed/23407381 http://dx.doi.org/10.3892/etm.2013.908 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
HAO, GUANGTAO
BAI, SHAOBO
LIANG, HAIXIA
LIANG, YUGUANG
QU, HENGYAN
GAO, HONGZHI
LI, YUANYUAN
ZHENG, ZHUANJIE
WANG, XIAOFANG
LIU, ZEYUAN
Development and validation of a HPLC-MS/MS method with electrospray ionization for quantitation of potassium oxonate in human plasma: Application to a pharmacokinetic study
title Development and validation of a HPLC-MS/MS method with electrospray ionization for quantitation of potassium oxonate in human plasma: Application to a pharmacokinetic study
title_full Development and validation of a HPLC-MS/MS method with electrospray ionization for quantitation of potassium oxonate in human plasma: Application to a pharmacokinetic study
title_fullStr Development and validation of a HPLC-MS/MS method with electrospray ionization for quantitation of potassium oxonate in human plasma: Application to a pharmacokinetic study
title_full_unstemmed Development and validation of a HPLC-MS/MS method with electrospray ionization for quantitation of potassium oxonate in human plasma: Application to a pharmacokinetic study
title_short Development and validation of a HPLC-MS/MS method with electrospray ionization for quantitation of potassium oxonate in human plasma: Application to a pharmacokinetic study
title_sort development and validation of a hplc-ms/ms method with electrospray ionization for quantitation of potassium oxonate in human plasma: application to a pharmacokinetic study
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3570248/
https://www.ncbi.nlm.nih.gov/pubmed/23407381
http://dx.doi.org/10.3892/etm.2013.908
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